Isolation and characterization of phycocyanins from the blue-green alga Spirulina platensis

Two main biliproteins c-phycocyanin and allophycocyanin were identified and characterized in the blue-green alga Spirulina platensis. The specific absorbance, fluorescence maxima, sub-unit make-up and amino acid composition of the biliproteins in Spirulina platensis resemble those reported for other blue-green algae. However, the minimum molecular weights (44,000 for c-phycocyanin and 38,000 for the allophycocyanin) and the specific extinction coefficients (73, and 58 for c-phycocyanin and allophycocyanin respectively) of these biliproteins were different from these values in other blue-green algae.

[1]  F. Eiserling,et al.  Characterization and structural properties of the major biliproteins of Anabaena sp. , 1976, Archives of Microbiology.

[2]  R. Menzi,et al.  Amino acid composition and nutritive valve of the alga Spirulina maxima. , 1967, Journal of the science of food and agriculture.

[3]  A. Glazer,et al.  Chromophore content of blue-green algal phycobiliproteins. , 1973, The Journal of biological chemistry.

[4]  B. Davis.  DISC ELECTROPHORESIS – II METHOD AND APPLICATION TO HUMAN SERUM PROTEINS * , 1964, Annals of the New York Academy of Sciences.

[5]  D. Berns,et al.  Physical-chemical properties of C-phycocyanin isolated from an acido-thermophilic eukaryote, Cyanidium caldarium. , 1975, The Biochemical journal.

[6]  D. M. Brown,et al.  Physico-chemical and immunological properties of allophycocyanins , 1977, Archives of Microbiology.

[7]  D. Berns,et al.  The characterization of C-phycocyanin from an extremely halo-tolerant blue-green alga, Coccochloris elabens. , 1973, The Biochemical journal.

[8]  A. Glazer.  Phycocyanins: Structure and Function , 1976 .

[9]  M. M. Allen,et al.  Nitrogen chlorosis in blue-green algae , 2004, Archiv für Mikrobiologie.

[10]  D. Berns.  Protein aggregation in phycocyanin--osmotic pressure studies. , 1970, Biochemical and biophysical research communications.

[11]  H. Zuber,et al.  C‐phycocyanin from the thermophilic blue‐green algaMastigocladus laminosus, isolation, characterization and subunit composition , 1972, FEBS letters.

[12]  A. Glazer,et al.  Subunit structure of the phycobiliproteins of blue-green algae. , 1971, Proceedings of the National Academy of Sciences of the United States of America.

[13]  J. Myers,et al.  RELATIONS BETWEEN PIGMENT CONTENT AND PHOTOSYNTHETIC CHARACTERISTICS IN A BLUE-GREEN ALGA , 1955, The Journal of general physiology.

[14]  L. Bogorad.  Phycobiliproteins and Complementary Chromatic Adaptation , 1975 .

[15]  S. Moore,et al.  [117] Chromatographic determination of amino acids by the use of automatic recording equipment , 1963 .

[16]  N. Carr,et al.  C-phycocyanin and allophycocyanin in two species of blue-green algae. , 1968, The Biochemical journal.

[17]  J. Myers,et al.  P.ELATIONS BETWEEN PIGMENT CONTENT AND PHOTOSYNTHETIC CHARACTERISTICS IN A , 1955 .