[The role of apoptosis and protective effect of carnitine in contralateral testicular injury in experimental unilateral testicular torsion].

BACKGROUND We examined the role of programmed cell death (PCD) in the etiology of contralateral testis injury as well as the protective effect of carnitine. METHODS Thirty-six Wistar Albino rats were used in the study. Rats were divided into six groups as control, sham, torsion-detorsion with 6- and 24-hour intervals, and torsion-detorsion-carnitine administration with 6- and 24-hour intervals. The left testes of all rats were torsioned 720 degrees. Intraperitoneal carnitine was administered to groups as 100 mg/kg one hour before detorsion. Orchiectomy was applied to the contralateral testis a week after detorsion. Testes were examined with respect to diameters of seminiferous tubules (DST), ratios of malonyl dialdehyde and Johnsen biopsy scores (JBS), and PCD ratios. Results were evaluated by ANOVA test. RESULTS Programmed cell death ratios were significantly higher in the torsion-detorsion with 6- and 24-hour intervals groups compared to the sham group. Those increases were more prominent in the torsion-detorsion with 24-hour interval group. DST and JBS of the control group were significantly higher than in the experimental groups (p < 0.05). The protective effect of carnitine was significant in the 24-hour torsion group, while no significant difference were detected in the 6-hour torsion-detorsion-carnitine group. CONCLUSION Increased injury and PCD in the contralateral testis was observed with prolonged exposure time in this model. It was possible to decrease the injury with carnitine.

[1]  M. Marberger,et al.  Experimental testicular torsion: Effect on endocrine and exocrine function and contralateral testicular histology , 1988, Urological Research.

[2]  M. Marberger,et al.  Hemodynamic effects of experimental testicular torsion , 2004, Urological Research.

[3]  Y. Hishikawa,et al.  Expression of Fas and Fas Ligand in Normal and Ischemia-Reperfusion Testes: Involvement of the Fas System in the Induction of Germ Cell Apoptosis in the Damaged Mouse Testis1 , 2001, Biology of reproduction.

[4]  O. Ozdemır,et al.  Increased cell proliferation and R.Msp1 fragmentation induced by 5-aza-2'-deoxycytidine in rat testes related to the gene imprinting mechanism. , 2000, Experimental and toxicologic pathology : official journal of the Gesellschaft fur Toxikologische Pathologie.

[5]  R. Gottlieb,et al.  Regulation of the activity of caspases by L‐carnitine and palmitoylcarnitine , 2000, FEBS letters.

[6]  F. Hadžiselimović,et al.  Increased apoptosis in the contralateral testes of patients with testicular torsion as a factor for infertility. , 1998, The Journal of urology.

[7]  P. Mazolewski,et al.  Effect of hyperbaric oxygen therapy on testicular ischemia-reperfusion injury. , 1998, The Journal of urology.

[8]  S. Moretti,et al.  EFFECT OF l-CARNITINE TREATMENT IN VIVO ON APOPTOSIS AND CERAMIDE GENERATION IN PERIPHERAL BLOOD LYMPHOCYTES FROM AIDS PATIENTS: CORRELATION WITH IN VITRO RESULTS , 1996 .

[9]  N. Büyükpamukçu,et al.  Tissue hypoxia in ipsilateral and contralateral testes undergoing surgically induced maldescent. , 1996, European journal of pediatric surgery : official journal of Austrian Association of Pediatric Surgery ... [et al] = Zeitschrift fur Kinderchirurgie.

[10]  M. Parvinen,et al.  In-situ quantification of stage-specific apoptosis in the rat seminiferous epithelium: effects of short-term experimental cryptorchidism. , 1995, International journal of andrology.

[11]  J. Koster.  Free radical-mediated damage and carnitine esters , 1995 .

[12]  J. Drago,et al.  Testicular torsion and its effects on contralateral testicle. , 1993, Urology.

[13]  M. Erkılıç,et al.  Contralateral testicular blood flow in unilateral testicular torsion measured by the 133Xe clearance technique. , 1992, British journal of urology.

[14]  I. Sesterhenn,et al.  Unilateral testicular torsion: abnormal histological findings in the contralateral testis--cause or effect? , 1990, British journal of urology.

[15]  S. Marklund,et al.  Morphologic changes induced by short-term ischemia in the rat testis are not affected by treatment with superoxide dismutase and catalase. , 1988, Journal of andrology.

[16]  T. Turner.  On unilateral testicular and epididymal torsion: no effect on the contralateral testis. , 1987, The Journal of urology.

[17]  J. Dormandy,et al.  Leukocytes and the risk of ischemic diseases. , 1987, JAMA.

[18]  B. A. Madarikan.  Testicular salvage following spermatic cord torsion. , 1987, Journal of pediatric surgery.

[19]  R. Cody,et al.  Identification of the direct vasodilator effect of milrinone with an isolated limb preparation in patients with chronic congestive heart failure. , 1986, Circulation.

[20]  R. Rabinowitz,et al.  Histological changes occurring in the contralateral testes of prepubertal rats subjected to various durations of unilateral spermatic cord torsion. , 1985, The Journal of urology.

[21]  W. L. Thomas,et al.  Diagnosis and outcome of testicular torsion , 1983, The British journal of surgery.

[22]  R. White,et al.  The effect of testicular torsion on the contralateral testis. , 1982, The Journal of urology.

[23]  G. Bartsch,et al.  Testicular torsion: late results with special regard to fertility and endocrine function. , 1980, The Journal of urology.

[24]  T. Krarup,et al.  The testes after torsion. , 1978, British journal of urology.

[25]  M. Macnicol.  Torsion of the testis in childhood , 1974, The British journal of surgery.

[26]  S. Johnsen.  Testicular biopsy score count--a method for registration of spermatogenesis in human testes: normal values and results in 335 hypogonadal males. , 1970, Hormones.