Micromixing-Enhanced Biosensing of Radioactivity Using Modified Deinococcus Radiodurans in Microfluidics

α-radiation is extremely harmful if inhaled or swallowed, as it can be easily cause carcinogenetic or other physiological diseases. Here, we develop a microfluidic radiation biosensing device based on the β-galactosidase generation of genetically modified bacteria Deinococcus Radiodurans PZ0423 (DRPZ423) upon exposure of the radiation. An electrokinetic bacteria trapper is configured to locally aggregate bacteria to enhance the sensitivity. Additionally, a micro-thick transparent silicone membrane (<20 μm) has been applied to contain the sensing bacteria with only an radiation reduction of <5 %. Moreover, a micro mixer was further integrated to shorten the sensing time and reduce the loss of p-aminophenol. Altogether, this work has demonstrated a novel microfluidic radiation biosensing technique for achieving reliable α-radiation monitoring.