Electrochemical detection in a microfluidic device of oxidative stress generated by macrophage cells.

The release of reactive oxygen species (ROS) or reactive nitrogen species (RNS), i.e., the initial phase of oxidative stress, by macrophage cells has been studied by electrochemistry within a microfluidic device. Macrophages were first cultured into a detection chamber containing the three electrodes system and were subsequently stimulated by the microinjection of a calcium ionophore (A23187). Their production of ROS and RNS was then measured by amperometry at the surface of a platinized microelectrode. The fabricated microfluidic device provides an accurate measurement of oxidative release kinetics with an excellent reproducibility. We believe that such a method is simple and versatile for a number of advanced applications based on the detection of biological processes of secretion by a few or even a single living cell.

[1]  V. Daniels,et al.  Oxidative damage and the preservation of organic artefacts. , 1989, Free radical research communications.

[2]  A. Ewing,et al.  Voltammetric measurement of oxygen in single neurons using platinized carbon ring electrodes. , 1992, Analytical chemistry.

[3]  T. Malinski,et al.  Nitric oxide release from a single cell measured in situ by a porphyrinic-based microsensor , 1992, Nature.

[4]  P. Cobbold,et al.  Bioluminescent measurement in single cardiomyocytes of sudden cytosolic ATP depletion coincident with rigor. , 1992, Journal of molecular and cellular cardiology.

[5]  J. A. Jankowski,et al.  Monitoring an oxidative stress mechanism at a single human fibroblast. , 1995, Analytical chemistry.

[6]  R. Kennedy,et al.  Detection of exocytosis at individual pancreatic beta cells by amperometry at a chemically modified microelectrode. , 1995, Proceedings of the National Academy of Sciences of the United States of America.

[7]  T. Iizuka,et al.  Direct measurement of oscillatory generation of superoxide anions by single phagocytes , 1996, FEBS letters.

[8]  Jonathan M. Cooper,et al.  Micromachining Sensors for Electrochemical Measurement in Subnanoliter Volumes , 1997 .

[9]  J. Piette,et al.  Phenylarsine oxide inhibits ex vivo HIV-1 expression. , 1997, Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie.

[10]  R S Foote,et al.  Microchip device for cell lysis, multiplex PCR amplification, and electrophoretic sizing. , 1998, Analytical chemistry.

[11]  J M Cooper,et al.  Single-cell measurements of purine release using a micromachined electroanalytical sensor. , 1998, Analytical chemistry.

[12]  G. Whitesides,et al.  Rapid Prototyping of Microfluidic Systems in Poly(dimethylsiloxane). , 1998, Analytical chemistry.

[13]  R. Wightman,et al.  Spatio-temporal resolution of exocytosis from individual cells. , 1998, Annual review of biophysics and biomolecular structure.

[14]  B. Herman,et al.  Measurement of intracellular calcium. , 1999, Physiological reviews.

[15]  A. Sarasin,et al.  Amplification of the Inflammatory Cellular Redox State by Human Immunodeficiency Virus Type 1-Immunosuppressive Tat and gp160 Proteins , 1999, Journal of Virology.

[16]  M. Erard,et al.  Analysis of individual biochemical events based on artificial synapses using ultramicroelectrodes: cellular oxidative burst. , 2000, Faraday discussions.

[17]  S. Quake,et al.  Monolithic microfabricated valves and pumps by multilayer soft lithography. , 2000, Science.

[18]  Y. Xian,et al.  Amperometric ultramicrosensors for peroxynitrite detection and its application toward single myocardial cells. , 2000, Analytical chemistry.

[19]  A. Ewing,et al.  Quantitative chemical analysis of single cells. , 2000, Annual review of biophysics and biomolecular structure.

[20]  Y Wakamoto,et al.  On-chip culture system for observation of isolated individual cells. , 2001, Lab on a chip.

[21]  Stephen R. Quake,et al.  A Microfabricated Rotary Pump , 2001 .

[22]  Paul Yager,et al.  Cell lysis and protein extraction in a microfluidic device with detection by a fluorogenic enzyme assay. , 2002, Analytical chemistry.

[23]  Mengsu Yang,et al.  Cell docking and on-chip monitoring of cellular reactions with a controlled concentration gradient on a microfluidic device. , 2002, Analytical chemistry.

[24]  S. Quake,et al.  A robust and scalable microfluidic metering method that allows protein crystal growth by free interface diffusion , 2002, Proceedings of the National Academy of Sciences of the United States of America.

[25]  G. FitzGerald,et al.  Oxidative Stress and Cardiovascular Injury: Part I: Basic Mechanisms and In Vivo Monitoring of ROS , 2003, Circulation.

[26]  M. McClain,et al.  Microfluidic devices for the high-throughput chemical analysis of cells. , 2003, Analytical chemistry.

[27]  J. Kutter,et al.  Integrating advanced functionality in a microfabricated high-throughput fluorescent-activated cell sorter. , 2003, Lab on a chip.

[28]  Aaron R Wheeler,et al.  Microfluidic device for single-cell analysis. , 2003, Analytical chemistry.

[29]  Christian Amatore,et al.  Oxidative stress in cancer prone xeroderma pigmentosum fibroblasts. Real-time and single cell monitoring of superoxide and nitric oxide production with microelectrodes. , 2003, Carcinogenesis.

[30]  J. Drapier,et al.  Monitoring in Real Time with a Microelectrode the Release of Reactive Oxygen and Nitrogen Species by a Single Macrophage Stimulated by its Membrane Mechanical Depolarization , 2006, Chembiochem : a European journal of chemical biology.