An automatic pulsed laser microfluorometer with high spatial and temporal resolution

The paper describes an automatic pulsed laser microfluorometer with high spatial and temporal resolution, developed in our laboratories. The instrument consists of: (i) a nitrogen‐laser‐pumped dye‐laser for the excitation of the fluorescence, (ii) a microscope with additional optics to focus the excitation beam on the sample and to collect the fluorescence, (iii) filters or monochromators to select the output wavelength, (iv) a fast photomultiplier tube to detect the signal, and (v) a dual time‐scale microprocessor‐controlled signal averager for the acquisition and processing of the signal.

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