Isolation and Biochemical Characterizations of Mid Gut Microbiota of Culex (Culex quinquefasciatus) Mosquitoes in Some Urban Sub Urban & Rural Areas of West Bengal.

Mosquitoes, in general are medically important vectors of many diseases like Malaria, Dengue and Filariasis, which are a great challenge for public health in many countries. All animals and plants establish symbiotic relationship with microbes. Mosquitoes can be considered as an holobiont units in which the host (mosquito) and its microbiota are involved in complex reciprocal multipartite interaction such as host reproduction and survival, protection against natural enemies. This naturally acquired microbial flora can modulate the mosquitos’ vectorial capacity by inhibiting the development of pathogen. But enough care has not been under taken regarding the biochemical characterization of Culex mosquitoes (Culex quinquefasciatus) in West Bengal. Therefore a preliminary investigation have been undertaken for the determination of biochemical characterization such as gram staining, pattern of growth, detection of economically important enzyme as well as antibiotic susceptibility assay of midgut bacterial isolates of Culex (Culex quinquefasciatus) in some urban, sub-urban and rural areas of West Bengal. Keyword: Culex, Gut-Microbiota, Biochemical Characteristics, Basal Immunity.

[1]  P. Rossi,et al.  Mosquito/microbiota interactions: from complex relationships to biotechnological perspectives. , 2012, Current opinion in microbiology.

[2]  A. Enayati,et al.  dentification of bacterial microflora in the midgut of the larvae and adult of wild aught Anopheles stephensi : A step toward finding suitable paratransgenesis andidates , 2011 .

[3]  G. Favia,et al.  Identification of the Midgut Microbiota of An. stephensi and An. maculipennis for Their Application as a Paratransgenic Tool against Malaria , 2011, PloS one.

[4]  E. Rosenberg,et al.  Symbiosis and development: the hologenome concept. , 2011, Birth defects research. Part C, Embryo today : reviews.

[5]  T. Fukatsu,et al.  Diversity and infection prevalence of endosymbionts in natural populations of the chestnut weevil: relevance of local climate and host plants , 2011, Molecular ecology.

[6]  T. Maoka,et al.  Symbiotic Bacterium Modifies Aphid Body Color , 2010, Science.

[7]  D. Segal,et al.  Commensal bacteria play a role in mating preference of Drosophila melanogaster , 2010, Proceedings of the National Academy of Sciences.

[8]  M. A. Berbert-Molina,et al.  Culture-dependent and culture-independent characterization of microorganisms associated with Aedes aegypti (Diptera: Culicidae) (L.) and dynamics of bacterial colonization in the midgut. , 2010, Acta tropica.

[9]  R. Bhatnagar,et al.  Bacterial diversity analysis of larvae and adult midgut microflora using culture-dependent and culture-independent methods in lab-reared and field-collected Anopheles stephensi-an Asian malarial vector , 2009, BMC Microbiology.

[10]  G. Dimopoulos,et al.  Implication of the Mosquito Midgut Microbiota in the Defense against Malaria Parasites , 2009, PLoS pathogens.

[11]  Y. Shouche,et al.  Studies on cultured and uncultured microbiota of wild culex quinquefasciatus mosquito midgut based on 16s ribosomal RNA gene analysis. , 2004, The American journal of tropical medicine and hygiene.

[12]  V. Dillon,et al.  The gut bacteria of insects: nonpathogenic interactions. , 2004, Annual review of entomology.

[13]  J. Beier,et al.  Bacterial population dynamics in three anopheline species: the impact on Plasmodium sporogonic development. , 1996, The American journal of tropical medicine and hygiene.