Application of ω1-decoupled 2D correlation spectra to the study of proteins

Abstract The utility of ω 1 decoupling for the elucidation of two-dimensional proton magnetic resonance spectra of proteins is demonstrated. The technique previously proposed for correlation spectroscopy (COSY) is analyzed and extensions to other types of two-dimensional spectroscopy such as spin-echo correlated spectroscopy (SECSY) and relayed correlation spectroscopy are proposed. It is further shown that by incorporating a z filter into the ω 1 -decoupled SECSY experiment, a two-dimensional spectrum is obtained in which all peaks are in phase and have absorption lineshapes in both dimensions.

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