论文信息 - Efficient site-specific cleavage of RNA using a terpyridine–copper(II) complex joined to a 2′-O-methyloligonucleotide by a non-flexible linker

Efficient site-specific cleavage of RNA using a terpyridine–copper(II) complex joined to a 2′-O-methyloligonucleotide by a non-flexible linker

A terpyridine–Cu(II) complex conjugated to an antisense 2′-O-methyloligonucleotide at the 5′-end cleaved RNA predominantly at the site opposite the 5′-end in moderate yield, but the cleavage yield increased by more than two-fold when a 2′-O-methyloligonucleotide was allowed to bind to the single-stranded region of the RNA-antisense complex.

[1] J. Bashkin,et al. Inorganic Mimics of Ribonucleases and Ribozymes: From Random Cleavage to Sequence-Specific Chemistry to Catalytic Antisense Drugs. , 1998, Chemical reviews.

[2] J. Sessler,et al. Metal Complex Conjugates of Antisense DNA Which Display Ribozyme-Like Activity , 1997 .

[3] J. Hall,et al. Towards artificial ribonucleases: the sequence-specific cleavage of RNA in a duplex. , 1996, Nucleic acids research.

[4] K. Guckian,et al. Experimental Measurement of Aromatic Stacking Affinities in the Context of Duplex DNA. , 1996, Journal of the American Chemical Society.

[5] L. Echegoyen,et al. Routes to Dendritic Networks: Bis‐Dendrimers by Coupling of Cascade Macromolecules through Metal Centers , 1995 .

[6] J. Sessler,et al. SITE-SPECIFIC HYDROLYSIS OF RNA BY EUROPIUM(III) TEXAPHYRIN CONJUGATED TO A SYNTHETIC OLIGODEOXYRIBONUCLEOTIDE , 1994 .

[7] J. Bashkin,et al. Sequence-Specific Cleavage of HIV mRNA by a Ribozyme Mimic , 1994 .

[8] J. Morrow,et al. Trivalent lanthanide ions do not cleave RNA in DNA-RNA hybrids , 1993 .

[9] E. Ohtsuka,et al. Secondary structure in formylmethionine tRNA influences the site-directed cleavage of ribonuclease H using chimeric 2'-O-methyl oligodeoxyribonucleotides. , 1990, Biochemistry.

[10] E. Ohtsuka,et al. Synthesis and hybridization studies on two complementary nona(2'-O-methyl)ribonucleotides. , 1987, Nucleic acids research.