Localization of corazonin in the nervous system of the cockroach Periplaneta americana

Antisera raised to the cardioactive peptide corazonin were used to localize immunoreactive cells in the nervous system of the American cockroach. Sera obtained after the seventh booster injection were sufficiently specific to be used for immunocytology. They recognized a subset of 10 lateral neurosecretory cells in the protocerebrum that project to, and arborize and terminate in the ipsilateral corpus cardiacum. They also reacted with bilateral neurons in each of the thoracic and abdominal neuromeres, a single dorsal unpaired median neuron in the suboesophageal ganglion, an interneuron in each optic lobe, and other neurons at the base of the optic lobe, in the tritocerebrum and deutocerebrum. The presence of corazonin in the abdominal neurons and the lateral neurosecretory cells was confirmed by HPLC fractionation of extracts of the abdominal ganglia, brains and retrocerebral complexes, followed by determination of corazonin by ELISA, which revealed in each tissue a single immunoreactive peak co-eluting with corazonin in two different HPLC systems. Antisera obtained after the first three booster injections recognized a large number of neuroendocrine cells and neurons in the brain and the abdominal nerve cord. However, the sera from the two rabbits reacted largely with different cells, indicating that the majority of this immunoreactivity was due to cross-reactivity. These results indicate that the production of highly specific antisera to some neuropeptides may require a considerable number of booster injections.

[1]  S. Tobe,et al.  Anatomy and electrophysiology of neurons terminating in the corpora allata of the cockroach Diploptera punctata , 1987, The Journal of comparative neurology.

[2]  S. Tobe,et al.  Neuroanatomy of the retrocerebral complex, in particular the pars intercerebralis and partes laterales in the cockroach Diploptera punctata eschscholtz (Dictyoptera : Blaberidae) , 1984 .

[3]  D. Swaab,et al.  Can specificity ever be proved in immunocytochemical staining. , 1977, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[4]  K. K. Chan,et al.  Allatostatin-immunoreactive neurons projecting to the corpora allata of adult Diploptera punctata , 1992, Cell and Tissue Research.

[5]  J. Truman,et al.  Identification of the Bursicon-Containing Neurones in Abdominal Ganglia of the Tobacco Hornworm, Manduca Sexta , 1982 .

[6]  R. Pipa.  Locations and central projections of neurons associated with the retrocerebral neuroendocrine complex of the cockroach Periplaneta americana (L.) , 1978, Cell and Tissue Research.

[7]  J. Veenstra.  Immunocytochemical demonstration of vertebrate peptides in invertebrates: The homology concept , 1988, Neuropeptides.

[8]  H. Keshishian,et al.  Identification and distribution of a proctolin‐like neuropeptide in the nervous system of the gypsy moth, Lymantria dispar, and in other lepidoptera , 1989, The Journal of comparative neurology.

[9]  S. Tobe,et al.  Primary structure of four allatostatins: neuropeptide inhibitors of juvenile hormone synthesis. , 1989, Proceedings of the National Academy of Sciences of the United States of America.

[10]  J. Hildebrand,et al.  Peptide‐immunocytochemistry of neurosecretory cells in the brain and retrocerebral complex of the sphinx moth Manduca sexta , 1991, The Journal of comparative neurology.

[11]  P. Evans.  Biogenic Amines in the Insect Nervous System , 1980 .

[12]  P. Braunig.  Suboesophageal DUM neurons innervate the principal neuropiles of the locust brain , 1991 .

[13]  C. Mason.  New features of the brain-retrocerebral neuroendocrine complex of the locust Schistocerca vaga (scudder) , 1973, Zeitschrift für Zellforschung und Mikroskopische Anatomie.

[14]  R. Feyereisen,et al.  Identification of an allatostatin from adult Diploptera punctata. , 1989, Biochemical and biophysical research communications.

[15]  H. Bennett,et al.  Purification of the two major forms of rat pituitary corticotropin using only reversed-phase liquid chromatography. , 1981, Biochemistry.

[16]  L. H. Rademakers.  Identification of a secretomotor centre in the brain of Locusta migratoria, controlling the secretory activity of the adipokinetic hormone producing cells of the corpus cardiacum , 1977, Cell and Tissue Research.

[17]  H. Pflüger,et al.  An identified dorsal unpaired median neurone and bilaterally projecting neurones exhibiting bovine pancreactic polypeptide-like/FMRFamide-like immunoreactivity in abdominal ganglia of the migratory locust , 2004, Cell and Tissue Research.

[18]  F. Kalish,et al.  Isolation and primary structure of two peptides with cardioacceleratory and hyperglycemic activity from the corpora cardiaca of Periplaneta americana. , 1984, Proceedings of the National Academy of Sciences of the United States of America.

[19]  I. Orchard.  Adipokinetic hormones—An update , 1987 .

[20]  J. Veenstra.  Presence of corazonin in three insect species, and isolation and identification of [His7]corazonin from Schistocerca americana , 1991, Peptides.

[21]  G. Gäde.  The adipokinetic hormone/red pigment-concentrating hormone peptide family: structures, interrelationships and functions. , 1990 .

[22]  J. Shabanowitz,et al.  Identity of a second type of allatostatin from cockroach brains: an octadecapeptide amide with a tyrosine-rich address sequence. , 1991, Proceedings of the National Academy of Sciences of the United States of America.

[23]  J. Veenstra.  Isolation and structure of corazonin, a cardioactive peptide from the American cockroach , 1989, FEBS letters.

[24]  J. Edwards,et al.  The projection of neuroendocrine fibers (NCC I and II) in the brains of three orthopteroid insects , 1980, Journal of morphology.

[25]  H. Nijhout.  Axonal pathways in the brain-retrocerebral neuroendocrine complex of Manduca sexta (L.) (Lepidoptera: Sphingidae) , 1975 .

[26]  J. C. Lodder,et al.  ACTH-Iike immunoreactivity in two electrotonically coupled giant neurons in the pond snail Lymnaea stagnalis , 1979, Cell and Tissue Research.

[27]  J. Veenstra,et al.  Phylogenetic differentiation of glandular cells in corpora cardiaca as studied immunocytochemically with region-specific antisera to adipokinetic hormone , 1987 .

[28]  J. Polak,et al.  Immunocytochemical localization of peptidergic neurons and neurosecretory cells in the neuro-endocrine system of the Colorado potato beetle with antisera to vertebrate regulatory peptides , 2004, Histochemistry.

[29]  M. Gersch,et al.  Purification and identification of neurohormone D, a heart accelerating peptide from the corpora cardiaca of the cockroach Periplaneta americana , 1982 .

[30]  J. C. Lodder,et al.  ACTH-like immunoreactivity in two electronically coupled giant neurons in the pond snail Lymnaea stagnalis. , 1979, Cell and tissue research.

[31]  H. Rowell.  The Cells of the Insect Neurosecretory System: Constancy, Variability, and the Concept of the Unique Identifiable Neuron , 1976 .

[32]  J. Truman,et al.  Metamorphosis of the cerebral neuroendocrine system in the moth Manduca sexta , 1986, The Journal of comparative neurology.

[33]  H. Penzlin,et al.  A new specific antibody reveals octopamine‐like immunoreactivity in cockroach ventral nerve cord , 1992, The Journal of comparative neurology.