Brief predator odour exposure activates the HPA axis independent of locomotor changes.

Although predator exposure has been proposed to elicit both behavioural responses and neuroendocrine effects in rodents, results of an increasing number studies have failed to consistently detect both of these alterations. We provide a detailed multivariate quantitative assessment of locomotor activity levels and plasma levels of adrenocorticotrophic hormone (ACTH) and corticosterone of male and female laboratory rats following brief (5 min) exposure to a predator odour, (2-propylthietane, the main constituent of weasel anal gland secretion). We show that there is a clear distinction between the behavioural and neuroendocrine responses, with predator odour inducing significant increases in corticosterone and ACTH levels without any significant accompanying changes in various locomotor activity measures.