Patterns of genetic variability in Brazilian Littorinids (Mollusca): a macrogeographic approach

Macrogeographic studies are important for understanding gene flow patterns, and comparative data for related species with distinct bionomical traits may help to clarify the importance of such traits in natural populations. The aims of this study were to quantify the genetic variability and the populational structuring of three Brazilian littorinid species (Nodilittorina lineolata, Littoraria flava and L. angulifera) and to discuss the relationship between them, as well as each species’ mode of development and spatial distribution. We also investigated the species diversity in the ziczac complex. Isozyme analyses were done on 20 samples of N. lineolata, nine of L. flava and 10 of L. angulifera, collected along 4000 km of the Brazilian coast. Sixteen polymorphic loci were analysed in N. lineolata, 15 in L. angulifera and 17 in L. flava. All species showed high genetic variability. At sites where more than one species was present, there was a correlation among the values of gene diversity.The degree of interpopulational differentiation (N. lineolata, FST = 0.028; L. flava, FST = 0.054; L. angulifera, FST = 0.185) was coherent with the mode of larval development of each species. No linkage disequilibrium was found in N. lineolata. These findings, together with morphological evidence, corroborated the existence of only one species of the ziczac complex along the Brazilian coast.

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