Improved Protocols for the Illumina Genome Analyzer Sequencing System

In this unit, we describe a set of improvements we have made to the standard Illumina Genome Analyzer protocols to make the sequencing process more reliable in a high‐throughput environment, reduce amplification bias, narrow the distribution of insert sizes, and reliably obtain high yields of data. Curr. Protoc. Hum. Genet. 62:18.2.1‐18.2.27. © 2009 by John Wiley & Sons, Inc.

[1]  International Human Genome Sequencing Consortium Finishing the euchromatic sequence of the human genome , 2004 .

[2]  E. Mardis The impact of next-generation sequencing technology on genetics. , 2008, Trends in genetics : TIG.

[3]  E. D. Hyman A new method of sequencing DNA. , 1988, Analytical biochemistry.

[4]  W. Gilbert,et al.  A new method for sequencing DNA. , 1977, Proceedings of the National Academy of Sciences of the United States of America.

[5]  F. Sanger,et al.  A Rapid Method for Determining Sequences in DNA by Primed Synthesis with DNA Polymerase , 1989 .

[6]  T. Hawkins,et al.  DNA purification and isolation using a solid-phase. , 1994, Nucleic acids research.

[7]  Nancy F. Hansen,et al.  Accurate Whole Human Genome Sequencing using Reversible Terminator Chemistry , 2008, Nature.

[8]  J. Sambrook,et al.  Molecular Cloning: A Laboratory Manual , 2001 .

[9]  M. Ronaghi,et al.  A Sequencing Method Based on Real-Time Pyrophosphate , 1998, Science.

[10]  Matthias Meyer,et al.  From micrograms to picograms: quantitative PCR reduces the material demands of high-throughput sequencing , 2007, Nucleic acids research.

[11]  Z. Xuan,et al.  Genome-wide in situ exon capture for selective resequencing , 2007, Nature Genetics.

[12]  G. Weinstock,et al.  Direct selection of human genomic loci by microarray hybridization , 2007, Nature Methods.

[13]  J. Riley,et al.  A novel, rapid method for the isolation of terminal sequences from yeast artificial chromosome (YAC) clones. , 1990, Nucleic acids research.

[14]  Professor Dr. Stefan Surzycki Basic Techniques in Molecular Biology , 2000, Springer Lab Manuals.

[15]  James R. Knight,et al.  Genome sequencing in microfabricated high-density picolitre reactors , 2005, Nature.

[16]  J. Marmur,et al.  [109] Use of ultraviolet absorbance-temperature profile for determining the guanine plus cytosine content of DNA , 1968 .

[17]  F. Sanger,et al.  DNA sequencing with chain-terminating inhibitors. , 1977, Proceedings of the National Academy of Sciences of the United States of America.

[18]  Richard Durbin,et al.  A large genome center's improvements to the Illumina sequencing system , 2008, Nature Methods.