Zebrafish sparse corresponds to an orthologue of c-kit and is required for the morphogenesis of a subpopulation of melanocytes, but is not essential for hematopoiesis or primordial germ cell development.

The relative roles of the Kit receptor in promoting the migration and survival of amniote melanocytes are unresolved. We show that, in the zebrafish, Danio rerio, the pigment pattern mutation sparse corresponds to an orthologue of c-kit. This finding allows us to further elucidate morphogenetic roles for this c-kit-related gene in melanocyte morphogenesis. Our analyses of zebrafish melanocyte development demonstrate that the c-kit orthologue identified in this study is required both for normal migration and for survival of embryonic melanocytes. We also find that, in contrast to mouse, the zebrafish c-kit gene that we have identified is not essential for hematopoiesis or primordial germ cell development. These unexpected differences may reflect evolutionary divergence in c-kit functions following gene duplication events in teleosts.

[1]  S. Voss,et al.  Genetic analysis of steel and the PG-M/versican-encoding gene AxPG as candidates for the white (d) pigmentation mutant in the salamander Ambystoma mexicanum , 1999, Development Genes and Evolution.

[2]  A. Force,et al.  Preservation of duplicate genes by complementary, degenerative mutations. , 1999, Genetics.

[3]  Y L Wang,et al.  Zebrafish hox clusters and vertebrate genome evolution. , 1998, Science.

[4]  S. Nishikawa,et al.  Stage-specific expression of the Kit receptor and its ligand (KL) during male gametogenesis in the mouse: a Kit-KL interaction critical for meiosis. , 1998, Development.

[5]  K. Vogel,et al.  Avian neural crest-derived neurogenic precursors undergo apoptosis on the lateral migration pathway. , 1998, Development.

[6]  S. Nishikawa,et al.  Transgene expression of steel factor in the basal layer of epidermis promotes survival, proliferation, differentiation and migration of melanocyte precursors. , 1998, Development.

[7]  H. Rodríguez-Martínez,et al.  Molecular basis for the dominant white phenotype in the domestic pig. , 1998, Genome research.

[8]  S. Lyman,et al.  c-kit ligand and Flt3 ligand: stem/progenitor cell factors with overlapping yet distinct activities. , 1998, Blood.

[9]  I. Jackson,et al.  Activation of the receptor tyrosine kinase Kit is required for the proliferation of melanoblasts in the mouse embryo. , 1997, Developmental biology.

[10]  M. Pesce,et al.  The c-kit receptor is involved in the adhesion of mouse primordial germ cells to somatic cells in culture , 1997, Mechanisms of Development.

[11]  N. Hopkins,et al.  Zebrafish vasa homologue RNA is localized to the cleavage planes of 2- and 4-cell-stage embryos and is expressed in the primordial germ cells. , 1997, Development.

[12]  V. Broudy,et al.  Stem cell factor and hematopoiesis. , 1997, Blood.

[13]  W. Pavan,et al.  Melanocyte development in vivo and in neural crest cell cultures: crucial dependence on the Mitf basic-helix-loop-helix-zipper transcription factor. , 1997, Development.

[14]  S. Smith,et al.  Spatial visualization of apoptosis using a whole-mount in situ DNA end-labeling technique. , 1997, BioTechniques.

[15]  G. Ciment,et al.  Autocrine regulation of neural crest cell development by steel factor. , 1997, Developmental biology.

[16]  B. Wehrle-Haller,et al.  Receptor tyrosine kinase-dependent neural crest migration in response to differentially localized growth factors. , 1997, BioEssays : news and reviews in molecular, cellular and developmental biology.

[17]  D A Kane,et al.  Characterization of zebrafish mutants with defects in embryonic hematopoiesis. , 1996, Development.

[18]  C. Nüsslein-Volhard,et al.  Zebrafish pigmentation mutations and the processes of neural crest development. , 1996, Development.

[19]  A. Schier,et al.  Hematopoietic mutations in the zebrafish. , 1996, Development.

[20]  C. Nüsslein-Volhard,et al.  Mutations affecting xanthophore pigmentation in the zebrafish, Danio rerio. , 1996, Development.

[21]  C. Kress,et al.  Spatial and temporal patterns of c-kit-expressing cells in WlacZ/+ and WlacZ/WlacZ mouse embryos. , 1996, Development.

[22]  D. Parichy.  Salamander pigment patterns: how can they be used to study developmental mechanisms and their evolutionary transformation? , 1996, The International journal of developmental biology.

[23]  G. Barsh,et al.  The genetics of pigmentation: from fancy genes to complex traits. , 1996, Trends in genetics : TIG.

[24]  D. Parichy.  Pigment patterns of larval salamanders (Ambystomatidae, Salamandridae): the role of the lateral line sensory system and the evolution of pattern-forming mechanisms. , 1996, Developmental biology.

[25]  J. A. Burch,et al.  KIT expression reveals a population of precursor melanocytes in human skin. , 1996, The Journal of investigative dermatology.

[26]  S. Nishikawa,et al.  Distinct stages of melanocyte differentiation revealed by anlaysis of nonuniform pigmentation patterns. , 1996, Development.

[27]  J. Postlethwait,et al.  Centromere-linkage analysis and consolidation of the zebrafish genetic map. , 1996, Genetics.

[28]  B. Schroeder,et al.  Multiple actions of stem cell factor in neural crest cell differentiation in vitro. , 1996, Developmental biology.

[29]  D. Ransom,et al.  Intraembryonic hematopoietic cell migration during vertebrate development. , 1995, Proceedings of the National Academy of Sciences of the United States of America.

[30]  S. Nishikawa,et al.  Effects of monoclonal anti-c-kit antibody (ACK2) on melanocytes in newborn mice. , 1995, The Journal of investigative dermatology.

[31]  C. Kimmel,et al.  Stages of embryonic development of the zebrafish , 1995, Developmental dynamics : an official publication of the American Association of Anatomists.

[32]  I. Mcniece,et al.  Stem cell factor , 1995, Journal of leukocyte biology.

[33]  S. Nishikawa,et al.  Steel factor directs melanocyte development in vitro through selective regulation of the number of c-kit+ progenitors. , 1995, Developmental biology.

[34]  N. L. Le Douarin,et al.  Steel and c‐kit in the development of avian melanocytes: A study of normally pigmented birds and of the hyperpigmented mutant silky fowl , 1995, Developmental dynamics : an official publication of the American Association of Anatomists.

[35]  K. Steel,et al.  Mutations at the W locus affect survival of neural crest-derived melanocytes in the mouse , 1995, Mechanisms of Development.

[36]  C. Baker,et al.  A Xenopus c-kit-related receptor tyrosine kinase expressed in migrating stem cells of the lateral line system , 1995, Mechanisms of Development.

[37]  B. Wehrle-Haller,et al.  Soluble and cell-bound forms of steel factor activity play distinct roles in melanocyte precursor dispersal and survival on the lateral neural crest migration pathway. , 1995, Development.

[38]  A. Bernstein,et al.  Expression of Xkl-1, a Xenopus gene related to mammalian c-kit, in dorsal embryonic tissue , 1995, Mechanisms of Development.

[39]  E. Dupin,et al.  Genetic and epigenetic control in neural crest development. , 1994, Current opinion in genetics & development.

[40]  G. Hauptmann,et al.  Two-color whole-mount in situ hybridization to vertebrate and Drosophila embryos. , 1994, Trends in genetics : TIG.

[41]  A. Schier,et al.  Efficient recovery of ENU-induced mutations from the zebrafish germline. , 1994, Genetics.

[42]  D. Raible,et al.  Restriction of neural crest cell fate in the trunk of the embryonic zebrafish. , 1994, Development.

[43]  C. Kimmel,et al.  Segment and cell type lineage restrictions during pharyngeal arch development in the zebrafish embryo. , 1994, Development.

[44]  T. Kinashi,et al.  Steel factor and c-kit regulate cell-matrix adhesion. , 1994, Blood.

[45]  C. Abboud,et al.  Stem cell factor regulates human melanocyte-matrix interactions. , 1994, Pigment cell research.

[46]  E. Huang,et al.  The kit-ligand (steel factor) and its receptor c-kit/W: pleiotropic roles in gametogenesis and melanogenesis. , 1993, Development (Cambridge, England). Supplement.

[47]  C. Erickson.  From the crest to the periphery: control of pigment cell migration and lineage segregation. , 1993, Pigment cell research.

[48]  E. Morii,et al.  Stem cell factor induces outgrowth of c-kit-positive neurites and supports the survival of c-kit-positive neurons in dorsal root ganglia of mouse embryos. , 1993, Development.

[49]  J. A. Weston,et al.  Transient steel factor dependence by neural crest-derived melanocyte precursors. , 1993, Developmental biology.

[50]  R. Perris,et al.  The role of cell-cell and cell-matrix interactions in the morphogenesis of the neural crest. , 1993, Developmental biology.

[51]  Kenneth M. Yamada,et al.  Contact stimulation of cell migration. , 1992, Journal of cell science.

[52]  S. Nishikawa,et al.  Requirement of c-kit for development of intestinal pacemaker system. , 1992, Development.

[53]  D. Williams,et al.  Steel factor is required for maintenance, but not differentiation, of melanocyte precursors in the neural crest. , 1992, Developmental biology.

[54]  Karen P. Steel,et al.  TRP-2/DT, a new early melanoblast marker, shows that steel growth factor (c-kit ligand) is a survival factor. , 1992, Development.

[55]  N. Hopkins,et al.  Production of germ-line chimeras in zebrafish by cell transplants from genetically pigmented to albino embryos. , 1992, Proceedings of the National Academy of Sciences of the United States of America.

[56]  K. Zsebo,et al.  The c-kit receptor ligand functions as a mast cell chemoattractant. , 1992, Blood.

[57]  S. Nishikawa,et al.  Necessity of extracellular domain of W (c-kit) receptors for attachment of murine cultured mast cells to fibroblasts. , 1992, Blood.

[58]  D. van der Kooy,et al.  Contiguous patterns of c-kit and steel expression: analysis of mutations at the W and Sl loci. , 1991, Development.

[59]  E. Morii,et al.  Characterization of Ws mutant allele of rats: a 12-base deletion in tyrosine kinase domain of c-kit gene. , 1991, Blood.

[60]  R. Spritz,et al.  Mutation of the KIT (mast/stem cell growth factor receptor) protooncogene in human piebaldism. , 1991, Proceedings of the National Academy of Sciences of the United States of America.

[61]  R. Bachvarova,et al.  Expression of c-kit encoded at the W locus of mice in developing embryonic germ cells and presumptive melanoblasts. , 1991, Developmental biology.

[62]  R. Ho,et al.  Cell-autonomous action of zebrafish spt-1 mutation in specific mesodermal precursors , 1990, Nature.

[63]  David A. Williams,et al.  Stem cell factor is encoded at the SI locus of the mouse and is the ligand for the c-kit tyrosine kinase receptor , 1990, Cell.

[64]  C. March,et al.  Molecular cloning of mast cell growth factor, a hematopoietin that is active in both membrane bound and soluble forms , 1990, Cell.

[65]  P. Leder,et al.  The hematopoietic growth factor KL is encoded by the SI locus and is the ligand of the c-kit receptor, the gene product of the W locus , 1990, Cell.

[66]  Y. Yarden,et al.  Developmental expression of c-kit, a proto-oncogene encoded by the W locus. , 1990, Development.

[67]  K. Nocka,et al.  Molecular bases of dominant negative and loss of function mutations at the murine c‐kit/white spotting locus: W37, Wv, W41 and W. , 1990, The EMBO journal.

[68]  S. Fraser,et al.  Pathways of trunk neural crest cell migration in the mouse embryo as revealed by vital dye labelling. , 1990, Development.

[69]  K. Nocka,et al.  The dominant W42 spotting phenotype results from a missense mutation in the c-kit receptor kinase. , 1990, Science.

[70]  W. Rice.  ANALYZING TABLES OF STATISTICAL TESTS , 1989, Evolution; international journal of organic evolution.

[71]  F. Ruddle,et al.  Primary structure of c‐kit: relationship with the CSF‐1/PDGF receptor kinase family–oncogenic activation of v‐kit involves deletion of extracellular domain and C terminus. , 1988, The EMBO journal.

[72]  R. Tucker,et al.  The control of pigment cell pattern formation in the California newt, Taricha torosa. , 1986, Journal of embryology and experimental morphology.

[73]  G. Streisinger,et al.  Segregation analyses and gene-centromere distances in zebrafish. , 1986, Genetics.

[74]  I. Iuchi,et al.  Erythropoiesis in the developing rainbow trout, Salmo gairdneri irideus: histochemical and immunochemical detection of erythropoietic organs. , 1983, The Journal of experimental zoology.

[75]  E. Russell,et al.  Analysis of pleiotropism at the dominant white-spotting (W) locus of the house mouse: a description of ten new W alleles. , 1981, Genetics.

[76]  R. Greenberg.  Biometry , 1969, The Yale Journal of Biology and Medicine.

[77]  M. C. Green,et al.  An experimental analysis of the pigment defect caused by mutations at the W and S1 loci in mice. , 1968, Developmental biology.

[78]  E. Russell,et al.  Gene-induced embryological modifications of primordial germ cells in the mouse. , 1957, The Journal of experimental zoology.

[79]  E. Russell,et al.  Quantitative analysis of the normal and four alternative degrees of an inherited macrocytic anemia in the house mouse. I. Number and size of erythrocytes. , 1951, Blood.

[80]  E. Russell.  Analysis of pleiotropism at the W-locus in the mouse; relationship between the effects of W and Wv substitution on hair pigmentation and on erythrocytes. , 1949, Genetics.

[81]  C. Little,et al.  The Occurrence of a Dominant Spotting Mutation in the House Mouse. , 1937, Proceedings of the National Academy of Sciences of the United States of America.

[82]  S. Aberle.  A study of the hereditary anaemia of mice , 1927 .

[83]  A. Groves,et al.  Neural crest diversification. , 1999, Current topics in developmental biology.

[84]  H. Broxmeyer,et al.  In vitro behavior of hematopoietic progenitor cells under the influence of chemoattractants: stromal cell-derived factor-1, steel factor, and the bone marrow environment. , 1998, Blood.

[85]  B. Schutte,et al.  Optimized conditions for cloning PCR products into an XcmI T-vector. , 1997, BioTechniques.

[86]  Stephen L. Johnson,et al.  Genetic control of adult pigment stripe development in zebrafish. , 1995, Developmental biology.

[87]  V. Nataf,et al.  Effect of the Steel gene product on melanogenesis in avian neural crest cell cultures. , 1994, Differentiation; research in biological diversity.

[88]  T. Hunter,et al.  Receptor protein-tyrosine kinases and their signal transduction pathways. , 1994, Annual review of cell biology.

[89]  J. A. Weston.  Sequential segregation and fate of developmentally restricted intermediate cell populations in the neural crest lineage. , 1991, Current topics in developmental biology.

[90]  C. Erickson.  Morphogenesis of the neural crest. , 1986, Developmental biology.

[91]  E. Russell.  Hereditary anemias of the mouse: a review for geneticists. , 1979, Advances in genetics.

[92]  Susan M. Smith,et al.  Direct In Situ End-Labeling for Detection of Apoptotic Cells in Tissue Sections , 2022 .