Detection of pesticide by polymeric enzyme electrodes.

Screen-printed electrodes (SPEs) containing immobilized acetylcholine esterase (AChE) enzyme were used for the electrochemical determination of organophosphorous (OP) and carbamate pesticides. The extent of AChE deactivation by the pesticide was determined in the presence of acetylcholine (AChCl) substrate. The unique nature of this approach lies in the enzyme immobilization procedure in which AChE was attached to the SPE by in situ bulk polymerization of acrylamide to ensure efficient adherence within the membrane with minimal losses in enzyme activity. Responses were observed for the pesticides Monocrotophos, Malathion, Metasystox and Lannate over the concentration range 0-10 ppb (microg L(-1)).

[1]  Jean-Louis Marty,et al.  Immobilization of acetylcholinesterase on screen-printed electrodes: comparative study between three immobilization methods and applications to the detection of organophosphorus insecticides , 2002 .

[2]  J. Barbash,et al.  TESTING WATER QUALITY FOR PESTICIDE POLLUTION , 1999 .

[3]  Anthony Turner,et al.  Application of dual-step potential on single screen-printed modified carbon paste electrodes for detection of amino acids and proteins , 1999 .

[4]  Róbert E. Gyurcsányi,et al.  Fast response potentiometric acetylcholine biosensor , 1999 .

[5]  Jean-Louis Marty,et al.  Enzyme immobilization procedures on screen-printed electrodes used for the detection of anticholinesterase pesticides Comparative study , 2004 .

[6]  R. Gilliom,et al.  Peer reviewed: testing water quality for pesticide pollution. , 1999, Environmental science & technology.

[7]  D. Barceló,et al.  Mobile phase variations in thermospray liquid chromatography-mass spectrometry of pesticides. , 1991, Journal of chromatography.

[8]  C. Tsou,et al.  Reactivation kinetics of diethylphosphoryl acetylcholine esterase. , 1985, European journal of biochemistry.

[9]  Vasif Hasirci,et al.  Construction of an acetylcholinesterase-choline oxidase biosensor for aldicarb determination. , 2002, Biosensors & bioelectronics.

[10]  J. Krejčí,et al.  Comparison of biosensoric and chromatographic methods for the detection of pesticides , 2005 .

[11]  Anthony Turner,et al.  Solvent-resistant carbon electrodes screen printed onto plastic for use in biosensors , 1997 .

[12]  R. Leidy,et al.  ELISA AND GC/MS ANALYSIS OF PESTICIDE RESIDUES IN NORTH CAROLINA 1 , 2000 .

[13]  Brian R. Eggins,et al.  Biosensors: An Introduction , 1997 .

[14]  Arben Merkoçi,et al.  Pesticide determination in tap water and juice samples using disposable amperometric biosensors made using thick-film technology , 2001 .

[15]  Till T Bachmann,et al.  Development, validation, and application of an acetylcholinesterase-biosensor test for the direct detection of insecticide residues in infant food. , 2002, Biosensors & bioelectronics.

[16]  A. C. Baillie,et al.  The biochemical mode of action of pesticides. , 1974 .

[17]  Jean-Louis Marty,et al.  Screen-printed electrode based on AChE for the detection of pesticides in presence of organic solvents. , 2002, Talanta.