Evaluation of the Airborne Bacterial Population in the Periodically Confined Antarctic Base Concordia

The environmental airborne bacterial population in relation to human confinement was investigated over a period of 1 year in the Concordia Research Station, which is located on the Eastern Antarctic plateau. The unique location of the station makes it suitable for different research domains such as glaciology, atmospheric sciences, astronomy, etc. Furthermore, it is used as a test bed for long-duration spaceflights to study the physiologic and psychological adaptation to isolated environments. A total of 96 samples were collected at eight different locations in the station at regular intervals. The airborne bacterial contamination was for 90% of the samples lower than 10.0 × 102 colony-forming units per cubic meter of air (CFU/m3) and the total bacterial contamination increased over time during confinement but diminished after re-opening of the base. Viable airborne bacteria with different morphology were identified by biochemical analyses. The predominant microflora was identified as Staphylococcus sp. (24.9% of total) and Bacillus sp. (11.6% of total) and was associated with human activity, but also environmental species such as Sphingomonas paucimobilis (belonging to the α-Proteobacteria) could establish themselves in the airborne population. A few opportunistic pathogens (6%) were also identified.

[1]  E. Mosley‐Thompson,et al.  Recovery and Identification of Viable Bacteria Immured in Glacial Ice , 2000 .

[2]  D L Pierson,et al.  Evaluation of portable air samplers for monitoring airborne culturable bacteria. , 2000, AIHAJ : a journal for the science of occupational and environmental health and safety.

[3]  Desmond J Lugg,et al.  Behavioral health in Antarctica: implications for long-duration space missions. , 2005, Aviation, space, and environmental medicine.

[4]  J. Dutkiewicz,et al.  Variability of airborne microflora in a hospital ward within a period of one year. , 2006, Annals of agricultural and environmental medicine : AAEM.

[5]  F. Rainey,et al.  Dust‐borne bacteria in animal sheds, schools and children's day care centres , 1999, Journal of applied microbiology.

[6]  M. Dramaix,et al.  Bacterial contamination of indoor air, surfaces, and settled dust, and related dust endotoxin concentrations in healthy office buildings. , 2005, Annals of agricultural and environmental medicine : AAEM.

[7]  S. Pinelli,et al.  Microbiological indoor air quality in healthy buildings. , 2002, The new microbiologica.

[8]  M. Salkinoja-Salonen,et al.  Sphingomonas aurantiaca sp. nov., Sphingomonas aerolata sp. nov. and Sphingomonas faeni sp. nov., air- and dustborne and Antarctic, orange-pigmented, psychrotolerant bacteria, and emended description of the genus Sphingomonas. , 2003, International journal of systematic and evolutionary microbiology.

[9]  M. Givskov,et al.  Quorum sensing in Serratia. , 2007, FEMS microbiology reviews.

[10]  K. Chiller,et al.  Skin microflora and bacterial infections of the skin. , 2001, The journal of investigative dermatology. Symposium proceedings.

[11]  Jacek Dutkiewicz,et al.  Bacterial and fungal aerosols in indoor environment in Central and Eastern European countries. , 2002, Annals of agricultural and environmental medicine : AAEM.

[12]  M. Devleeschouwer,et al.  Characteristics of the home bacterial contamination and endotoxin related release. , 2006, Journal de pharmacie de Belgique.

[13]  P. Grisoli,et al.  Determination of aerial microbiological contamination in scholastic sports environments , 2003, Journal of applied microbiology.

[14]  Cover Sheet.  DROPLET FATE IN INDOOR ENVIRONMENTS , OR CAN WE PREVENT THE SPREAD OF INFECTION ? , 2007 .

[15]  Maosheng Yao,et al.  Analysis of Portable Impactor Performance for Enumeration of Viable Bioaerosols , 2007, Journal of occupational and environmental hygiene.

[16]  J Barker,et al.  The potential spread of infection caused by aerosol contamination of surfaces after flushing a domestic toilet , 2005, Journal of applied microbiology.

[17]  Chihshan Li,et al.  Analysis of Bioaerosols from Chicken Houses by Culture and Non-Culture Method , 2006 .

[18]  Jack Stuster.  Analogue prototypes for Lunar and Mars exploration. , 2005, Aviation, space, and environmental medicine.

[19]  N. Pace,et al.  Culture-Independent Molecular Analysis of Microbial Constituents of the Healthy Human Outer Ear , 2003, Journal of Clinical Microbiology.

[20]  L. Beaugerie,et al.  Microbial-gut interactions in health and disease. Antibiotic-associated diarrhoea. , 2004, Best practice & research. Clinical gastroenterology.

[21]  R. Brief,et al.  Indoor Pollution: Guidelines for Prevention and Control of Microbiological Respiratory Hazards Associated with Air Conditioning and Ventilation Systems , 1988 .

[22]  D. Pierson,et al.  Microbial Characterization during the Early Habitation of the International Space Station , 2004, Microbial Ecology.

[23]  J. Aislabie,et al.  Aromatic hydrocarbon-degrading bacteria from soil near Scott Base, Antarctica , 2000, Polar Biology.

[24]  N. D. Novikova,et al.  Review of the Knowledge of Microbial Contamination of the Russian Manned Spacecraft , 2004, Microbial Ecology.

[25]  Nikolai Polikarpov,et al.  Survey of environmental biocontamination on board the International Space Station. , 2006, Research in microbiology.

[26]  L. Beaugerie,et al.  Antibiotic-associated diarrhoea , 2004 .

[27]  L. Palinkas.  The psychology of isolated and confined environments. Understanding human behavior in Antarctica. , 2003, The American psychologist.

[28]  I. Szczerba.  [Occurrence and number of bacteria from the Micrococcus, Kocuria, Nesterenkonia, Kytococcus and Dermacoccus genera on skin and mucous membranes in humans]. , 2003, Medycyna doswiadczalna i mikrobiologia.

[29]  Joseph Ofungwu,et al.  Indoor Air Quality Investigation and Health Risk Assessment at Correctional Institutions , 2005, Integrated environmental assessment and management.

[30]  P. Hsueh,et al.  Nosocomial infections caused by Sphingomonas paucimobilis: clinical features and microbiological characteristics. , 1998, Clinical infectious diseases : an official publication of the Infectious Diseases Society of America.

[31]  G. Berg.  Diversity of antifungal and plant‐associated Serratia plymuthica strains , 2000, Journal of applied microbiology.

[32]  Y. C. Lin,et al.  Storage effects on bacterial concentration: determination of impinger and filter samples. , 2001, The Science of the total environment.

[33]  Novikova Nd,et al.  Current problems of microbial safety of the interior environment of orbital stations after extended period of operation , 1995 .