Poly(lactide-co-glycolide) nanoparticles, layer by layer engineered for the sustainable delivery of antiTNF-α.

A strategy of encapsulation of the antiTNF-α antibody on top of poly(lactide-co-glycolide) nanoparticles (PLGA NPs) is presented on the basis of the complexation of antiTNF-α with alginate (Alg) and subsequent assembly layer by layer with poly(L-lysine) (PLL). The assembly of the antiTNF-α/Alg complex with PLL and its stability in PBS and lysozymes are monitored on a planar support using a quartz crystal microbalance with dissipation. The assembly of the antiTNF-α/Alg complex on PLGA NPs is followed by zeta potential measurements. AntiTNF-α release from the PLGA NPs is measured in PBS at 37 and 60 °C and in the HepG2 cell line following NP uptake, using the Q-ADA kit detection kit. The release follows first-order kinetics with an initial burst. Intracellular release of antiTNF-α is confirmed by confocal Raman microscopy.

[1]  A. Jonas,et al.  Ordered polyelectrolyte "multilayers". 1. Mechanisms of growth and structure formation: A comparison with classical fuzzy "multilayers" , 2001 .

[2]  C Vigneron,et al.  Influence of experimental parameters on the characteristics of poly(lactic acid) nanoparticles prepared by a double emulsion method. , 1998, Journal of controlled release : official journal of the Controlled Release Society.

[3]  A. Lowman,et al.  Biodegradable nanoparticles for drug delivery and targeting , 2002 .

[4]  C Vigneron,et al.  Preparation and characterization of protein C-loaded PLA nanoparticles. , 1999, Journal of controlled release : official journal of the Controlled Release Society.

[5]  Yi Yan Yang,et al.  Effect of preparation temperature on the characteristics and release profiles of PLGA microspheres containing protein fabricated by double-emulsion solvent extraction/evaporation method. , 2000, Journal of controlled release : official journal of the Controlled Release Society.

[6]  N. Moroz,et al.  Encapsulation of proteins by layer-by-layer adsorption of polyelectrolytes onto protein aggregates: factors regulating the protein release. , 2001, Biotechnology and bioengineering.

[7]  D. Irvine,et al.  Layer-by-layer-assembled multilayer films for transcutaneous drug and vaccine delivery. , 2009, ACS nano.

[8]  E. Allémann,et al.  Development of a nanoprecipitation method intended for the entrapment of hydrophilic drugs into nanoparticles. , 2005, European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences.

[9]  Jayanth Panyam,et al.  The use of nanoparticle-mediated targeted gene silencing and drug delivery to overcome tumor drug resistance. , 2010, Biomaterials.

[10]  R Langer,et al.  New methods of drug delivery. , 1990, Science.

[11]  R. Gurny,et al.  In Vitro Extended-Release Properties of Drug-Loaded Poly(DL-Lactic Acid) Nanoparticles Produced by a Salting-Out Procedure , 1993, Pharmaceutical Research.

[12]  Obesity: A high dose of JuNK for fat mice , 2003, Nature Reviews Drug Discovery.

[13]  T. Mizushima,et al.  Techniques for efficient entrapment of pharmaceuticals in biodegradable solid micro/nanoparticles , 2010, Expert opinion on drug delivery.

[14]  Ulrich S. Schubert,et al.  Synthetic polymeric nanoparticles by nanoprecipitation , 2009 .

[15]  J. Kempeni.  Preliminary results of early clinical trials with the fully human anti-TNFα monoclonal antibody D2E7 , 1999, Annals of the rheumatic diseases.

[16]  K. Shakesheff,et al.  Polymeric systems for controlled drug release. , 1999, Chemical reviews.

[17]  H. M. Nielsen,et al.  Spray drying of siRNA-containing PLGA nanoparticles intended for inhalation , 2009, Journal of Controlled Release.

[18]  Gero Decher,et al.  Fuzzy Nanoassemblies: Toward Layered Polymeric Multicomposites , 1997 .

[19]  Jie Zhou,et al.  Surface engineered Poly(lactide-co-glycolide) nanoparticles for intracellular delivery: uptake and cytotoxicity--a confocal raman microscopic study. , 2010, Biomacromolecules.

[20]  S. Dubas,et al.  Mechanism of Polyelectrolyte Multilayer Growth: Charge Overcompensation and Distribution , 2001 .

[21]  Irantzu Llarena,et al.  Stepwise surface tailoring of carbon nanotubes with polyelectrolyte brushes and lipid layers to control their intracellular distribution and ``in vitro'' toxicity , 2011 .

[22]  Xudong Yuan,et al.  SiRNA drug delivery by biodegradable polymeric nanoparticles. , 2006, Journal of nanoscience and nanotechnology.

[23]  Periodontics: Controlled release , 2004, British Dental Journal.

[24]  Hatem Fessi,et al.  Nanocapsule formation by interfacial polymer deposition following solvent displacement , 1989 .

[25]  Frank Caruso,et al.  Investigation of the influence of polyelectrolyte charge density on the growth of multilayer thin films prepared by the layer-by-layer technique , 2002 .

[26]  Johannes Schmitt,et al.  Buildup of ultrathin multilayer films by a self-assembly process: III. Consecutively alternating adsorption of anionic and cationic polyelectrolytes on charged surfaces , 1992 .

[27]  R. Levy,et al.  Sustained delivery and expression of DNA encapsulated in polymeric nanoparticles , 2000, Gene Therapy.

[28]  Y. Israel,et al.  Ethanol increases tumor necrosis factor-alpha receptor-1 (TNF-R1) levels in hepatic, intestinal, and cardiac cells. , 2004, Alcohol.

[29]  J. Schlenoff,et al.  Factors Controlling the Growth of Polyelectrolyte Multilayers , 1999 .

[30]  J. Hofmann,et al.  Defoliation and plasmid delivery with layer-by-layer coated colloids. , 2006, Macromolecular bioscience.

[31]  P. Tak,et al.  Tumor necrosis factor antagonist mechanisms of action: a comprehensive review. , 2008, Pharmacology & therapeutics.

[32]  Stanislav Emelianov,et al.  Poly(Lactic-co-Glycolic) Acid as a Carrier for Imaging Contrast Agents , 2009, Pharmaceutical Research.

[33]  Gleb B. Sukhorukov,et al.  Reversible pH-Dependent Properties of Multilayer Microcapsules Made of Weak Polyelectrolytes , 2004 .

[34]  A. Coombes,et al.  The preparation of sub-200 nm poly(lactide-co-glycolide) microspheres for site-specific drug delivery , 1993 .

[35]  F. Breedveld,et al.  Therapeutic monoclonal antibodies , 2000, The Lancet.

[36]  A. Voigt,et al.  Polyelectrolyte complexes and layer-by-layer capsules from chitosan/chitosan sulfate. , 2002, Biomacromolecules.

[37]  O'donnell,et al.  Preparation of microspheres by the solvent evaporation technique. , 1997, Advanced drug delivery reviews.