Improvements of nanometer particle's measuring system based on photon correlation spectroscopy

The measuring system based on photon correlation spectroscopy is improved through several means. The distribution of nanometer particle's sizes measured by new systems is more stable and accurate. All of the experiments are done in an ultra-clean chamber. The temperature is controlled and changes from about 13 degrees centigrade to 22 degrees centigrade. Fibers with different core diameters are used to transmit scattering light. The "Y" type fibers with different core diameters are used to transmit both the incident laser and the scattering light. The microscope objectives with different numerical apertures are used to collect and couple the scattering light into fiber. The software of real time correlation is tried to be used in the measuring system and it is compared with the static correlation. The Labview is used to integrate the software of correlation and inverse algorithms of nanometer particle sizes. Influences of incident laser with different power and mode are analyzed.

[1]  Jerker Widengren,et al.  Interactions and Kinetics of Single Molecules as Observed by Fluorescence Correlation Spectroscopy , 1993 .

[2]  Xiaoyi Bao,et al.  Polarization-dependent loss autocorrelation in the presence of combined polarization-mode dispersion and polarization-dependent losses in optical fibers , 2003, Other Conferences.

[3]  LI Hong-xia.  Fiber-optic systems for dynamic light scattering , 2007 .

[4]  M. Eigen,et al.  Sorting single molecules: application to diagnostics and evolutionary biotechnology. , 1994, Proceedings of the National Academy of Sciences of the United States of America.

[5]  H. Rigneault,et al.  Fluorescence correlation spectroscopy on a mirror , 2003 .

[6]  R. Pecora,et al.  Dynamic Light Scattering: Applications of Photon Correlation Spectroscopy , 2011 .

[7]  J. Lippincott-Schwartz,et al.  Studying protein dynamics in living cells , 2001, Nature Reviews Molecular Cell Biology.

[8]  W. Webb,et al.  Thermodynamic Fluctuations in a Reacting System-Measurement by Fluorescence Correlation Spectroscopy , 1972 .

[9]  S. Provencher.  CONTIN: A general purpose constrained regularization program for inverting noisy linear algebraic and integral equations , 1984 .

[10]  Lie-Jane Kao.  The temporal autocorrelation in FCS: a single-molecule detection nano-biotechnology , 2005, SPIE Optics East.

[11]  Qi Zhang,et al.  Experimental studies of PCS system for measuring of nanometer particles , 2009, International Conference on Optical Instruments and Technology.

[12]  Lars Edman,et al.  FCS in Single Molecule Analysis , 2001 .

[13]  Watt W Webb,et al.  Biological and chemical applications of fluorescence correlation spectroscopy: a review. , 2002, Biochemistry.

[14]  Lloyd M. Davis,et al.  Dealing with reduced data acquisition times in Fluorescence Correlation Spectroscopy (FCS) for High-Throughput Screening (HTS) applications , 2003, SPIE BiOS.

[15]  R. Rigler,et al.  Fluorescence correlations, single molecule detection and large number screening. Applications in biotechnology. , 1995, Journal of biotechnology.

[16]  S. Aragon,et al.  Fluorescence correlation spectroscopy as a probe of molecular dynamics , 1976 .

[17]  Svetlana A. Tatarkova,et al.  Application of fluorescence correlation spectroscopy for drug delivery to tumor tissue , 2000, European Conference on Biomedical Optics.

[18]  B. Frisken,et al.  Revisiting the method of cumulants for the analysis of dynamic light-scattering data. , 2001, Applied optics.

[19]  E. Elson,et al.  Fluorescence correlation spectroscopy. I. Conceptual basis and theory , 1974 .

[20]  S. Provencher.  A constrained regularization method for inverting data represented by linear algebraic or integral equations , 1982 .

[21]  P. Huibers.  Models for the wavelength dependence of the index of refraction of water. , 1997, Applied optics.

[22]  Joerg Enderlein,et al.  Art and artifacts of fluorescence correlation spectroscopy , 2005, SPIE BiOS.

[23]  Michal Neeman,et al.  Combined use of fluorescent and dynamic light scattering imaging for applications in vascular biology , 2008, SPIE BiOS.

[24]  L. Brand,et al.  High-content data evaluation by means of confocal fluorescence spectroscopy , 2005, SPIE BiOS.

[25]  R. Rigler,et al.  Fluorescence correlation spectroscopy , 2001 .