Multiparameter flow‐cytometrical quantitation of circulating CD34+‐cells: correlation to the quantitation of circulating haemopoietic progenitor cells by in vitro colony‐assay

Summary. A multiparameter flow‐cytometrical method for the quantitation of CD34+‐cells present in adult human peripheral blood cells (PBMC) has been developed. PBMC from 13 healthy adult subjects were analysed for CD34+‐cells by flow‐cytometry, with only the lymphoid population of cells negative for anti‐CD3‐moAb included in the analysis. At the same time mononuclear cells from the same individuals were depleted of CD3+‐ and CDl4+‐cells by immunomagnetic purging. These cells were cultured for haemopoietic colonies. A correlation coefficient of 0·92 was calculated by regression analysis of CD34+‐cells number versus the numbers of colonies (CFU‐GM, BFU‐E).

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