Rabbit tooth pulp compared to 55°C mouse hot plate assay for detection of antinociceptive activity of opiate and nonopiate central analgesics

The rabbit tooth pulp (RTP) assay was compared to the 55°C mouse hot plate (MHP) assay for detecting the antinociceptive actions of carfentanil, sufentanil, fentanyl, alfentanil, meperidine, morphine, ketamine, and nefopam. Except for meperidine, all the the opioid agonists exhibited lower ED50 values (P ≤ 0.01) from RTP. Meperidine exhibited a lower value, significant at P ≤ 0.10. Rank orders of potencies were the same for both assys, with carfentanil the most and morphine the least potent. The durations of action of single equianalgesic doses were significantly longer for all six opioids when determined by the RTP compared to the MHP assay. Rank orders of duration were similar in both assys, with alfentanil the shortest and morphine the longest. Graded dose responses could not be detected for ketamine and nefopam using the MHP, but they were observed and ED50 values were obtained with the RTP assay. The results indicate that the RTP is singnificantly more sensitive than the MHP assay in detecting antinociceptive activity of six oplate and two nonopiate central analgesics.

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