Functional PLGA scaffolds for chondrogenesis of bone-marrow-derived mesenchymal stem cells.

Two chondrogenic factors, Dex and TGF-beta1, were incorporated into PLGA scaffolds and their chondrogenic potential was evaluated. The Dex-loaded PLGA scaffold was grafted with AA and heparin, the heparin-immobilized one was then reacted with TGF-beta1, yielding a PLGA/Dex-TGF (PLGA/D/T) scaffold. The scaffolds were seeded with rabbit MSCs and cultured for 4 weeks. The results show that the scaffolds including chondrogenic factors strongly upregulated the expression of cartilage-specific genes and clearly displayed type-II collagen immunofluorescence. The functionalized PLGA scaffolds could provide an appropriate niche for chondrogenic differentiation of MSC without a constant medium supply of Dex and TGF-beta1.

[1]  C. M. Agrawal,et al.  Biodegradable polymeric scaffolds for musculoskeletal tissue engineering. , 2001, Journal of biomedical materials research.

[2]  Morimichi Mizuno,et al.  Chondrogenic differentiation of bovine bone marrow mesenchymal stem cells in pellet cultural system. , 2004, Experimental hematology.

[3]  Won Ho Park,et al.  Electrospinning of silk fibroin nanofibers and its effect on the adhesion and spreading of normal human keratinocytes and fibroblasts in vitro. , 2004, Biomaterials.

[4]  R. Reis,et al.  Plasma- and chemical-induced graft polymerization on the surface of starch-based biomaterials aimed at improving cell adhesion and proliferation , 2003, Journal of materials science. Materials in medicine.

[5]  Antonios G. Mikos,et al.  Injectable biodegradable hydrogel composites for rabbit marrow mesenchymal stem cell and growth factor delivery for cartilage tissue engineering. , 2007, Biomaterials.

[6]  D. Hutmacher,et al.  In vivo mesenchymal cell recruitment by a scaffold loaded with transforming growth factor beta1 and the potential for in situ chondrogenesis. , 2002, Tissue engineering.

[7]  Bernd Baumann,et al.  Chondrogenic differentiation of human mesenchymal stem cells in collagen type I hydrogels. , 2007, Journal of biomedical materials research. Part A.

[8]  J. Vacanti,et al.  Tissue engineering : Frontiers in biotechnology , 1993 .

[9]  D. Buttle,et al.  Improved quantitation and discrimination of sulphated glycosaminoglycans by use of dimethylmethylene blue. , 1986, Biochimica et biophysica acta.

[10]  Ling Qin,et al.  Porous gelatin-chondroitin-hyaluronate tri-copolymer scaffold containing microspheres loaded with TGF-beta1 induces differentiation of mesenchymal stem cells in vivo for enhancing cartilage repair. , 2006, Journal of biomedical materials research. Part A.

[11]  N. Kawazoe,et al.  Chondrogenic differentiation of mesenchymal stem cells in a leakproof collagen sponge , 2008 .

[12]  B. Gupta,et al.  Plasma-induced graft polymerization of acrylic acid onto poly(ethylene terephthalate) films: characterization and human smooth muscle cell growth on grafted films. , 2001, Biomaterials.

[13]  Boon Chin Heng,et al.  Combined effects of TGFβ1 and BMP2 in serum-free chondrogenic differentiation of mesenchymal stem cells induced hyaline-like cartilage formation , 2005, Growth factors.

[14]  Byung-Soo Kim,et al.  Enhancement of ectopic bone formation by bone morphogenetic protein-2 released from a heparin-conjugated poly(L-lactic-co-glycolic acid) scaffold. , 2007, Biomaterials.

[15]  M. Pittenger,et al.  Multilineage potential of adult human mesenchymal stem cells. , 1999, Science.

[16]  Robert Langer,et al.  Controlled‐release of IGF‐I and TGF‐β1 in a photopolymerizing hydrogel for cartilage tissue engineering , 2001, Journal of orthopaedic research : official publication of the Orthopaedic Research Society.

[17]  Y. Ito,et al.  Synthesis and antithrombogenicity of anionic polyurethanes and heparin-bound polyurethanes. , 1986, Journal of biomedical materials research.

[18]  N Pallua,et al.  Modulation of angiogenic potential of collagen matrices by covalent incorporation of heparin and loading with vascular endothelial growth factor. , 2004, Tissue engineering.

[19]  W. Sebald,et al.  Human bone morphogenetic protein 2 contains a heparin-binding site which modifies its biological activity. , 1996, European journal of biochemistry.

[20]  E. Kroon,et al.  Production of pancreatic hormone–expressing endocrine cells from human embryonic stem cells , 2006, Nature Biotechnology.

[21]  T. Hsien,et al.  Preparation and characterization of RGD-immobilized chitosan scaffolds. , 2005, Biomaterials.

[22]  J. Williams,et al.  Effect of transforming growth factor beta1 on chondrogenic differentiation of cultured equine mesenchymal stem cells. , 2000, American journal of veterinary research.

[23]  D. Mooney,et al.  Polymeric system for dual growth factor delivery , 2001, Nature Biotechnology.

[24]  D. Heymann,et al.  Recent advances in TGF-β effects on chondrocyte metabolism , 2002 .

[25]  Jia-cong Shen,et al.  Surface modification of poly‐L‐lactide by photografting of hydrophilic polymers towards improving its hydrophilicity , 2002 .

[26]  D. Mooney,et al.  Regulating activation of transplanted cells controls tissue regeneration. , 2006, Proceedings of the National Academy of Sciences of the United States of America.

[27]  R. Tuan,et al.  Glucocorticoids Promote Chondrogenic Differentiation of Adult Human Mesenchymal Stem Cells by Enhancing Expression of Cartilage Extracellular Matrix Genes , 2006, Stem cells.

[28]  R. Tuan,et al.  Chondrogenic differentiation and functional maturation of bovine mesenchymal stem cells in long-term agarose culture. , 2006, Osteoarthritis and cartilage.

[29]  Kwideok Park,et al.  Preparation of Biodegradable Polymer Scaffolds with Dual Pore System for Tissue Regeneration , 2007 .

[30]  J. Aubin,et al.  Differentiation of muscle, fat, cartilage, and bone from progenitor cells present in a bone-derived clonal cell population: effect of dexamethasone , 1988, The Journal of cell biology.

[31]  Robert J Fisher,et al.  Heparin-regulated release of growth factors in vitro and angiogenic response in vivo to implanted hyaluronan hydrogels containing VEGF and bFGF. , 2006, Biomaterials.

[32]  Jeffrey T Borenstein,et al.  Microfabrication of three-dimensional engineered scaffolds. , 2007, Tissue engineering.

[33]  David G Simpson,et al.  Utilizing acid pretreatment and electrospinning to improve biocompatibility of poly(glycolic acid) for tissue engineering. , 2004, Journal of biomedical materials research. Part B, Applied biomaterials.