Mini Review: Cytochrome P450BM-3 in the Remediation of Semi-Volatile Fatty Acids Associated with Feedlot Odors

A sound understanding of Animal Feeding Operations (AFOs) air emissions and their effects require the expertise of many disciplines. These disciplines may include a broad representation of academia, industry and local communities. Bioengineering is an emerging field that can provide new remediation methodologies by addressing the problem of chemical odor emissions from CAFO's. Development of methods for the reduction of organic compounds has the ability to move from monitoring and detection to air quality improvement. Utilization of metabolic pathways and the heterologous protein expression of Saccharomyces cerevisiae can yield cost effective methods for odor management at CAFOs. The metabolism of volatile fatty acids (VFAs) using Saccharomyces cerevisiae produced cytochrome P450BM-3 could possibly reduce downwind odors and assist in beneficial biogas production in wastewater effluents. The purpose of this review is to discuss a multiple disciplinary approach to air quality combining a variety of approaches which can assist federal agencies in successfully implementing a long term plan for this issue.

[1]  M. Bazin,et al.  Microbial population dynamics , 1982 .

[2]  K. McMahon,et al.  Anaerobic codigestion of municipal solid waste and biosolids under various mixing conditions--I. Digester performance. , 2001, Water research.

[3]  John T. Groves.  Oxygen activation and transfer catalyzed by ruthenium porphyrins , 1990 .

[4]  R D Schmid,et al.  P450 in biotechnology: zinc driven omega-hydroxylation of p-nitrophenoxydodecanoic acid using P450 BM-3 F87A as a catalyst. , 2000, Journal of biotechnology.

[5]  Jarett P. Spinhirne,et al.  Multidimensional gas chromatography-olfactometry for the identification and prioritization of malodors from confined animal feeding operations. , 2005, Journal of agricultural and food chemistry.

[6]  J Deisenhofer,et al.  Crystal structure of hemoprotein domain of P450BM-3, a prototype for microsomal P450's. , 1993, Science.

[7]  L. Narhi,et al.  Characterization of a catalytically self-sufficient 119,000-dalton cytochrome P-450 monooxygenase induced by barbiturates in Bacillus megaterium. , 1986, The Journal of biological chemistry.

[8]  Janusz Pawliszyn,et al.  System for the generation of standard gas mixtures of volatile and semi-volatile organic compounds for calibrations of solid-phase microextraction and other sampling devices. , 2004, Journal of chromatography. A.

[9]  Michael D. Aitken,et al.  Characterization of reaction products from the enzyme catalyzed oxidation of phenolic pollutants , 1994 .

[10]  James A Nicell,et al.  Detoxification of phenolic solutions with horseradish peroxidase and hydrogen peroxide. , 2002, Water research.

[11]  矢野 貴人,et al.  Directed Evolution のさまざまな応用例 , 2003 .

[12]  J. Mason,et al.  Structure-function analysis of the bacterial aromatic ring-hydroxylating dioxygenases. , 1997, Advances in microbial physiology.

[13]  R D Schmid,et al.  P450 monooxygenase in biotechnology. I. Single-step, large-scale purification method for cytochrome P450 BM-3 by anion-exchange chromatography. , 1999, Journal of chromatography. A.

[14]  K. McMahon,et al.  Anaerobic codigestion of municipal solid waste and biosolids under various mixing conditions--II: Microbial population dynamics. , 2001, Water research.

[15]  M. J. Coon,et al.  Cytochrome P-450 : multiplicity of isoforms, substrates, and catalytic and regulatory mechanisms , 1991 .

[16]  D W Nebert,et al.  P450 superfamily: update on new sequences, gene mapping, accession numbers and nomenclature. , 1996, Pharmacogenetics.

[17]  James A. Nicell,et al.  Enzyme catalyzed polymerization and precipitation of aromatic compounds from aqueous solution , 1993 .

[18]  David B. Parker,et al.  Odor Characterization at Open-Lot Beef Cattle Feedyards Using Triangular Forced Choice Olfactometry , 2003 .

[19]  Eric T. Boder,et al.  Yeast surface display for screening combinatorial polypeptide libraries , 1997, Nature Biotechnology.

[20]  R D Schmid,et al.  Directed evolution of the fatty-acid hydroxylase P450 BM-3 into an indole-hydroxylating catalyst. , 2000, Chemistry.

[21]  Ortiz de Montellano,et al.  Cytochrome P-450: Structure, Mechanism, and Biochemistry , 1986 .

[22]  J Deisenhofer,et al.  Structure and function of cytochromes P450: a comparative analysis of three crystal structures. , 1995, Structure.

[23]  T. Poulos,et al.  The structure of the cytochrome p450BM-3 haem domain complexed with the fatty acid substrate, palmitoleic acid , 1997, Nature Structural Biology.