Proliferation and adhesion capability of human gingival fibroblasts onto zirconia, lithium disilicate and feldspathic veneering ceramic in vitro.

Human gingival fibroblasts (HGFs) were cultured onto CAD/CAM zirconia (Group A), CAD/CAM zirconia after polishing (Group B), CAD/CAM lithium disilicate after polishing (Group C), and feldspathic ceramic (Group D) to evaluate their proliferation and adhesion potential. After 3 h, HGF adhesion was similar in all groups. Later, HGFs closely adhered to surfaces, particularly onto groups B, C and D, acquiring an elongated shape. Proliferation assay showed no differences in cell viability among the groups after 24 h, while significant increase was shown after 72 h in Groups B and C. After 24 h, similar Collagen I levels were found in all groups, while after 72 h Groups B and C revealed a deep reduction in respect to the 24 h level. In vitro, HGF behavior may reflect variability in soft tissue response to different surface materials for prosthetic restorations, and support that polished zirconia is able to achieve a better integration in vivo in respect to the other materials.

[1]  Yong-Dae Kwon,et al.  Attachment and growth behaviour of human gingival fibroblasts on titanium and zirconia ceramic surfaces , 2009, Biomedical materials.

[2]  R. Messer,et al.  In vitro cytotoxicity of traditional versus contemporary dental ceramics. , 2003, The Journal of prosthetic dentistry.

[3]  S. Madihally,et al.  Increased matrix synthesis by fibroblasts with decreased proliferation on synthetic chitosan–gelatin porous structures , 2012, Biotechnology and bioengineering.

[4]  A. Puriene,et al.  In vitro evaluation of cytotoxicity of permanent prosthetic materials. , 2011, Stomatologija.

[5]  Roberto Sorrentino,et al.  From porcelain-fused-to-metal to zirconia: clinical and experimental considerations. , 2011, Dental materials : official publication of the Academy of Dental Materials.

[6]  A. Piattelli,et al.  The effect of material characteristics, of surface topography and of implant components and connections on soft tissue integration: a literature review. , 2006, Clinical oral implants research.

[7]  M. Roos,et al.  Two-body wear of monolithic, veneered and glazed zirconia and their corresponding enamel antagonists. , 2013, Acta odontologica Scandinavica.

[8]  A. Surazynski,et al.  Differential effects of echistatin and thrombin on collagen production and prolidase activity in human dermal fibroblasts and their possible implication in beta1-integrin-mediated signaling. , 2005, Pharmacological research.

[9]  L. Griffith,et al.  Capturing complex 3D tissue physiology in vitro , 2006, Nature Reviews Molecular Cell Biology.

[10]  A. Cataldi,et al.  Human gingival fibroblasts stress response to HEMA: A role for protein kinase C α. , 2013, Journal of biomedical materials research. Part A.

[11]  S. Tetè,et al.  Clinical evaluation of 209 all-ceramic single crowns cemented on natural and implant-supported abutments with different luting agents: a 6-year retrospective study. , 2012, Clinical implant dentistry and related research.

[12]  C. Sukotjo,et al.  The influence of different implant materials on human gingival fibroblast morphology, proliferation, and gene expression. , 2011, The International journal of oral & maxillofacial implants.

[13]  Susanne Stampf,et al.  Prospective clinical split-mouth study of pressed and CAD/CAM all-ceramic partial-coverage restorations: 7-year results. , 2013, The International journal of prosthodontics.

[14]  L. Valandro,et al.  Impact of surface finishes on the flexural strength and fracture toughness of In-Ceram Zirconia. , 2012, General dentistry.

[15]  Kenneth M. Yamada,et al.  Taking Cell-Matrix Adhesions to the Third Dimension , 2001, Science.

[16]  M. Brackett,et al.  In vitro cytotoxic response to lithium disilicate dental ceramics. , 2008, Dental materials : official publication of the Academy of Dental Materials.

[17]  S. Tetè,et al.  Five-year prospective clinical study of posterior three-unit zirconia-based fixed dental prostheses , 2011, Clinical Oral Investigations.

[18]  T. Albrektsson,et al.  On implant surfaces: a review of current knowledge and opinions. , 2010, The International journal of oral & maxillofacial implants.

[19]  D. Brunette,et al.  A light and electron microscopic study of the effects of surface topography on the behavior of cells attached to titanium-coated percutaneous implants. , 1991, Journal of biomedical materials research.

[20]  Danielle M Layton,et al.  A systematic review and meta-analysis of the survival of non-feldspathic porcelain veneers over 5 and 10 years. , 2013, The International journal of prosthodontics.

[21]  D. Layton,et al.  The up to 21-year clinical outcome and survival of feldspathic porcelain veneers: accounting for clustering. , 2012, The International journal of prosthodontics.

[22]  R. G. Craig,et al.  Precision of and new methods for testing in vitro alloy cytotoxicity. , 1992, Dental materials : official publication of the Academy of Dental Materials.

[23]  A. Cataldi,et al.  2-Hydroxyethyl methacrylate inflammatory effects in human gingival fibroblasts. , 2013, International endodontic journal.

[24]  M. Missori,et al.  Growth, viability, adhesion potential, and fibronectin expression in fibroblasts cultured on zirconia or feldspatic ceramics in vitro. , 2008, Journal of biomedical materials research. Part A.

[25]  K. Koyano,et al.  The difference of fibroblast behavior on titanium substrata with different surface characteristics , 2011, Odontology.

[26]  C. Lohmann,et al.  Response of MG63 osteoblast-like cells to titanium and titanium alloy is dependent on surface roughness and composition. , 1998, Biomaterials.

[27]  Danielle M Layton,et al.  A systematic review and meta-analysis of the survival of feldspathic porcelain veneers over 5 and 10 years. , 2014, The International journal of prosthodontics.

[28]  I. Reaney,et al.  In vitro biocompatibility of modified potassium fluorrichterite and potassium fluorrichterite-fluorapatite glass–ceramics , 2011, Journal of materials science. Materials in medicine.

[29]  A. Guillouzo,et al.  General review on in vitro hepatocyte models and their applications. , 2010, Methods in molecular biology.

[30]  A. Uehara,et al.  Functional TLRs and NODs in Human Gingival Fibroblasts , 2007, Journal of dental research.

[31]  P. Kramer,et al.  Integrin mediated attachment of periodontal ligament to titanium surfaces. , 2009, Dental materials : official publication of the Academy of Dental Materials.

[32]  M. Baldoni,et al.  A study on the attachment of human gingival cell structures to oral implant materials. , 1991, The International journal of prosthodontics.

[33]  J. Kivilahti,et al.  Effect of surface processing on the attachment, orientation, and proliferation of human gingival fibroblasts on titanium. , 1992, Journal of biomedical materials research.

[34]  E. Angelis,et al.  Adaptive Response of Osteoblasts Grown on a Titanium Surface: Morphology, Cell Proliferation and Stress Protein Synthesis , 2005, Veterinary Research Communications.

[35]  T. Albrektsson,et al.  Histologic comparison of ceramic and titanium implants in cats. , 1990, The International journal of oral & maxillofacial implants.