Standard operating procedures (SOP) in experimental stroke research: SOP for middle cerebral artery occlusion in the mouse

Recently, systematic reviews have found quantitative evidence that low study quality may have introduced a bias into preclinical stroke research. Monitoring, auditing, and standard operating procedures (SOPs) are already key elements of quality control in randomized clinical trials and will hopefully be widely adopted by preclinical stroke research in the near future. Increasingly, funding bodies and review boards overseeing animal experiments are taking a proactive stance, and demand auditable quality control measures in preclinical research. Every good quality control system is based on its SOPs. This article introduces the concept of quality control and presents for the first time an SOP in experimental stroke research.

[1]  U. Dirnagl,et al.  Empirical Evidence of Bias in the Design of Experimental Stroke Studies: A Metaepidemiologic Approach , 2008, Stroke.

[2]  Stroke Therapy Academic Industry Roundtable.  Recommendations for standards regarding preclinical neuroprotective and restorative drug development. , 1999, Stroke.

[3]  K. Reymann,et al.  Pattern of time-dependent reduction of histologically determined infarct volume after focal ischaemia in mice , 2008, Neuroscience Letters.

[4]  Marc Fisher,et al.  Update of the Stroke Therapy Academic Industry Roundtable Preclinical Recommendations , 2009, Stroke.

[5]  S. Doré,et al.  Use of an optimized transient occlusion of the middle cerebral artery protocol for the mouse stroke model. , 2006, Journal of stroke and cerebrovascular diseases : the official journal of National Stroke Association.

[6]  G. Donnan,et al.  Systematic Review and Metaanalysis of the Efficacy of FK506 in Experimental Stroke , 2005, Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism.

[7]  Markus Schwaninger,et al.  Improving Outcome after Stroke: Overcoming the Translational Roadblock , 2008, Cerebrovascular Diseases.

[8]  U. Dirnagl,et al.  Stroke research at a road block: the streets from adversity should be paved with meta‐analysis and good laboratory practice , 2009, British journal of pharmacology.

[9]  U. Dirnagl.  Bench to Bedside: The Quest for Quality in Experimental Stroke Research , 2006, Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism.

[10]  P. Bath,et al.  Effects of NXY‐059 in experimental stroke: an individual animal meta‐analysis , 2009, British journal of pharmacology.

[11]  U. Dirnagl,et al.  Evidence for the Efficacy of NXY-059 in Experimental Focal Cerebral Ischaemia Is Confounded by Study Quality , 2008, Stroke.