Evaluation of glutathione-binding protein A of Haemophilus parasuis as a vaccine candidate in a mouse model

The virulent strains of Haemophilus parasuis are the causative agents of Glässer’s disease, which can cause systemic infection and result in polyserositis, meningitis and arthritis. The development of novel, effective vaccines would be beneficial to preventing H. parasuis infections. Here, we report a novel immunogenic protein, glutathione-binding protein A (GbpA), which can elicit a significant humoral antibody response and confer significant protection against challenge with a lethal dose of a highly virulent H. parasuis strain. The H. parasuis strain can be fully eliminated in the immunized mice. The results indicate that GbpA has the potential to be used as an effective component of a new vaccine against H. parasuis.

[1]  Shuai Song,et al.  Identification of secreted proteins as novel antigenic vaccine candidates of Haemophilus parasuis serovar 5. , 2015, Vaccine.

[2]  T. Nicholson,et al.  Virulence, Transmission, and Heterologous Protection of Four Isolates of Haemophilus parasuis , 2013, Clinical and Vaccine Immunology.

[3]  Huanchun Chen,et al.  Immunogenicity and protective efficacy of recombinant Haemophilus parasuis SH0165 putative outer membrane proteins. , 2013, Vaccine.

[4]  F. Yuan,et al.  The Live Attenuated Actinobacillus pleuropneumoniae Triple-Deletion Mutant ΔapxIC ΔapxIIC ΔapxIV-ORF1 Strain, SLW05, Immunizes Pigs against Lethal Challenge with Haemophilus parasuis , 2012, Clinical and Vaccine Immunology.

[5]  F. Yuan,et al.  Evaluation of recombinant proteins of Haemophilus parasuis strain SH0165 as vaccine candidates in a mouse model. , 2012, Research in veterinary science.

[6]  S. Savvides,et al.  Delineation of the Pasteurellaceae-specific GbpA-family of glutathione-binding proteins , 2011, BMC Biochemistry.

[7]  S. Savvides,et al.  Glutathione import in Haemophilus influenzae Rd is primed by the periplasmic heme-binding protein HbpA , 2010, Proceedings of the National Academy of Sciences.

[8]  Huanchun Chen,et al.  Identification and characterization of novel immunogenic outer membrane proteins of Haemophilus parasuis serovar 5. , 2009, Vaccine.

[9]  À. Olvera,et al.  Update on the diagnosis of Haemophilus parasuis infection in pigs and novel genotyping methods. , 2007, Veterinary journal.

[10]  F. MacIver,et al.  Glutathione is an essential metabolite required for resistance to oxidative stress in the yeastSaccharomyces cerevisiae , 1996, Current Genetics.

[11]  Hideyuki Suzuki,et al.  The yliA, -B, -C, and -D Genes of Escherichia coli K-12 Encode a Novel Glutathione Importer with an ATP-Binding Cassette , 2005, Journal of bacteriology.

[12]  M. Vaneechoutte,et al.  Exogenous Glutathione Completes the Defense against Oxidative Stress in Haemophilus influenzae , 2003, Journal of bacteriology.

[13]  H. Amano,et al.  Pathologic observations of pigs intranasally inoculated with serovar 1, 4 and 5 of Haemophilus parasuis using immunoperoxidase method. , 1994, The Journal of veterinary medical science.

[14]  R. Nielsen.  Pathogenicity and Immunity Studies of Haemophilus Parasuis Serotypes , 1993, Acta Veterinaria Scandinavica.

[15]  V. Rapp-Gabrielson,et al.  Designation of 15 serovars of Haemophilus parasuis on the basis of immunodiffusion using heat-stable antigen extracts , 1992, Journal of clinical microbiology.

[16]  Rapp-Gabrielson Vj,et al.  Comparative virulence of Haemophilus parasuis serovars 1 to 7 in guinea pigs. , 1992, American journal of veterinary research.

[17]  D. Gabrielson,et al.  Comparative virulence of Haemophilus parasuis serovars 1 to 7 in guinea pigs. , 1992, American journal of veterinary research.

[18]  O. P. Miniats,et al.  Vaccination of gnotobiotic primary specific pathogen-free pigs against Haemophilus parasuis. , 1991, Canadian journal of veterinary research = Revue canadienne de recherche veterinaire.

[19]  O. P. Miniats,et al.  Preliminary assessment of a Haemophilus parasuis bacterin for use in specific pathogen free swine. , 1989, Canadian journal of veterinary research = Revue canadienne de recherche veterinaire.