[Characterization and application of a monoclonal antibody against light chain of goose immunoglobulin].

Immunoglobulin (Ig) is considered a part of the innate immune system and cooperates with the complementary system as the first line of defense. In this study, a monoclonal antibody (MAb) direct against the light chain of goose Ig (GoIgCL) was generated, characterized and identified in various immunoassays to detect goose Ig. An immunoaffinity chromatography column prepared with this MAb was used to separate the goose Ig from sera. After being conjugated with horseradish peroxidase (HRP), this MAb was used as the secondary antibody to evaluate the goose-specific antibody. In addition, this MAb distinguished and localized the SIg+ lymphocytes from peripheral blood lymphocytes. MAb against GoIgCL may be good candidate to detect or purify goose Ig under various conditions and as a powerful tool for humoral immunity research on goose.

[1]  Bo Ma,et al.  Development and characterization of monoclonal antibodies against goose CD3ɛ extracellular domain and their application in detection of CD3(+) T lymphocytes. , 2015, Veterinary immunology and immunopathology.

[2]  Bo Ma,et al.  Development and evaluation of a competitive ELISA using a monoclonal antibody for antibody detection after goose parvovirus virus-like particles (VLPs) and vaccine immunization in goose sera. , 2014, Journal of virological methods.

[3]  Panpan Zhao,et al.  Molecular characterization and B cell membrane expression analysis of Fc fragment gene of goose IgY. , 2014, Research in veterinary science.

[4]  Panpan Zhao,et al.  Generation and characterization of polyclonal antibody against part of immunoglobulin constant heavy υ chain of goose. , 2014, Monoclonal antibodies in immunodiagnosis and immunotherapy.

[5]  Bo Ma,et al.  A novel monoclonal antibody against the constant region of goose immunoglobulin light chain. , 2014, Monoclonal antibodies in immunodiagnosis and immunotherapy.

[6]  M. Suresh,et al.  Diagnostics of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) nucleocapsid antigen using chicken immunoglobulin Y , 2012, Poultry Science.

[7]  Richard O'Kennedy,et al.  Affinity chromatography as a tool for antibody purification. , 2012, Methods.

[8]  M. Li,et al.  An ELISA based on the repeated foot-and-mouth disease virus 3B epitope peptide can distinguish infected and vaccinated cattle , 2012, Applied Microbiology and Biotechnology.

[9]  W. Zhan,et al.  Monoclonal antibodies recognizing mucus immunoglobulin and surface immunoglobulin-positive cells of flounder (Paralichthys olivaceus). , 2012, Veterinary immunology and immunopathology.

[10]  Bo Ma,et al.  Goose parvovirus structural proteins expressed by recombinant baculoviruses self-assemble into virus-like particles with strong immunogenicity in goose. , 2011, Biochemical and biophysical research communications.

[11]  B. Kaspers,et al.  Evaluation of Newcastle disease virus immunoassays for waterfowl using a monoclonal antibody specific for the duck immunoglobulin light chain , 2008, Avian pathology : journal of the W.V.P.A.

[12]  Yuan Zhang,et al.  Purification of GFP fusion proteins with high purity and yield by monoclonal antibody-coupled affinity column chromatography. , 2008, Protein expression and purification.

[13]  Richard R Burgess,et al.  Antigen-binding properties of monoclonal antibodies reactive with human TATA-binding protein and use in immunoaffinity chromatography. , 2004, Protein expression and purification.

[14]  F. Haesebrouck,et al.  Monoclonal and polyclonal antibodies to chicken immunoglobulin isotypes specifically detect turkey immunoglobulin isotypes. , 1997, Veterinary immunology and immunopathology.

[15]  D. Allen,et al.  Analysis of the expression and secretion of isotypes of sheep B cell immunoglobulins with a panel of isotype-specific monoclonal antibodies. , 1995, Research in veterinary science.

[16]  V. Vetvicka,et al.  Natural antibody and complement-mediated antigen processing and presentation by B lymphocytes. , 1994, Journal of immunology.

[17]  B. Kaspers,et al.  Development of specific enzyme-linked immunosorbent antibody assay systems for the detection of chicken immunoglobulins G, M, and A using monoclonal antibodies. , 1992, Poultry science.

[18]  H. Ambrosius,et al.  Surface immunoglobulin on lymphocytes of the tortoise Agrionemys horsfieldii. , 1989, Developmental and comparative immunology.

[19]  J. Beatty,et al.  Measurement of monoclonal antibody affinity by non-competitive enzyme immunoassay. , 1987, Journal of immunological methods.

[20]  J. Meece,et al.  Birds, migration and emerging zoonoses: west nile virus, lyme disease, influenza A and enteropathogens. , 2003, Clinical medicine & research.

[21]  A. Subramanian.  Immunoaffinity chromatography , 2000, Methods in molecular biology.

[22]  A. L. Brassfield.  Antigen purification by monoclonal antibody immunoaffinity chromatography. , 1995, Methods in molecular biology.

[23]  R. Haugland.  Coupling of monoclonal antibodies with enzymes. , 1995, Methods in molecular biology.

[24]  R. Haugland.  Coupling of monoclonal antibodies with fluorophores. , 1995, Methods in molecular biology.