Effects of nutrients on the production of AK-111-81 macrolide antibiotic by Streptomyces hygroscopicus.

The influence of different carbon and nitrogen sources on the production of AK-111-81 nonpolyenic macrolide antibiotic by Streptomyces hygroscopicus 111-81 was studied. Substitution of glucose with lactose or glycerol significantly affected maximal antibiotic AK-111-81 productivity as the growth rate was close to that of the basal fermentation medium. Addition of ammonium succinate to the fermentation medium markedly increased the antibiotic productivity as the growth rate was low. Divalent ions as Mn2+, Cu2+, Fe2+ stimulated AK-111-81 antibiotic biosynthesis. These results allow us to develop a new fermentation medium showing 6-fold increase of AK-111-81 antibiotic formation compared with the basal fermentation medium.

[1]  Sergio Sánchez,et al.  Metabolic regulation of fermentation processes , 2002 .

[2]  K. Chater,et al.  Cloning and transcription analysis of the entire glycerol utilization (gylABX) operon of Streptomyces coelicolor A3(2) and identification of a closely associated transcription unit , 2004, Molecular and General Genetics MGG.

[3]  A. Demain,et al.  Carbon Catabolite Regulation of Cephalosporin Production in Streptomyces clavuligerus , 1978, Antimicrobial Agents and Chemotherapy.

[4]  L. Vining,et al.  Biochemistry and genetic regulation of commercially important antibiotics , 1983 .

[5]  M. Arias,et al.  Effect of different nutrients on the production of polyene antibiotics PA-5 and PA-7 by Streptoverticillium sp 43/16 in chemically defined media , 1988, Applied Microbiology and Biotechnology.

[6]  C. Schaffner,et al.  Factors Affecting the Production of Candicidin , 1975, Antimicrobial Agents and Chemotherapy.

[7]  Y. Aharonowitz.  Nitrogen metabolite regulation of antibiotic biosynthesis. , 1980, Annual review of microbiology.

[8]  L. Vining,et al.  Nutritional control of actinorhodin production byStreptomyces coelicolor A3(2): suppressive effects of nitrogen and phosphate , 2004, Applied Microbiology and Biotechnology.

[9]  L. Vining,et al.  Nutrient utilization in actinomycetes. Induction of α-glucosidases in Streptomyces venezuelae , 1981 .

[10]  J F Martin,et al.  Control of antibiotic biosynthesis. , 1980, Microbiological reviews.

[11]  A. Demain,et al.  Effect of nitrogen source on biosynthesis of rapamycin by Streptomyces hygroscopicus , 1997, Journal of Industrial Microbiology and Biotechnology.

[12]  V. Vinci,et al.  Improvement of microbial strains and fermentation processes , 2000, Applied Microbiology and Biotechnology.

[13]  S Coisne,et al.  Actinorhodin production by Streptomyces coelicolor A3(2) in iron‐restricted media , 1999, Letters in applied microbiology.

[14]  A. Demain,et al.  Effect of amino acids on rapamycin biosynthesis by Streptomyces hygroscopicus , 1995, Applied Microbiology and Biotechnology.

[15]  Ahmed Lebrihi,et al.  Effect of ammonium ions on spiramycin biosynthesis in Streptomyces ambofaciens , 1992, Applied Microbiology and Biotechnology.

[16]  L. Katz,et al.  The loading domain of the erythromycin polyketide synthase is not essential for erythromycin biosynthesis in Saccharopolyspora erythraea. , 1998, Microbiology.