Genome-wide association study to identify soybean stem pushing resistance and lodging resistance loci

Abstract: Lodging resistance is an important objective for soybean [Glycine max (L.) Merr.] breeding, but selection for this trait has been difficult since the resistance is controlled by multiple genes, and these genes interact with the environment. A total of 130 of 139 lines constituting a soybean genome-wide association study panel were phenotyped for stem pushing resistance, which is defined as the push-back strength when the plant stem is inclined, by measuring the force required to push a stem to a 45° angle using a force gauge in a greenhouse, and also for lodging, plant height, seed yield, and maturity at three locations in total in eastern Canada in 2013 or 2017. Two quantitative trait loci (QTLs) for pushing resistance were identified on chromosome 5 and 11, and each QTL accounted for 16.0% of phenotypic variation. In our panel, the alleles for higher pushing resistance were always of lower frequency than the alternate allele. Examining the panel at these QTLs identified that higher pushing resistance was associated with lower lodging on chromosomes 5 and 11, and that the difference for lodging between alleles was significant on chromosome 5. There was no difference in plant height or yield at the QTL on chromosomes 5 or 11, while higher pushing resistance was associated with later maturity at both QTLs. The pushing resistance QTL on chromosome 11 will be useful for decreasing lodging in Canadian short-season soybean.

[1]  Baohui Liu,et al.  A new dominant locus, E11, controls early flowering time and maturity in soybean , 2019, Molecular Breeding.

[2]  F. Taguchi-Shiobara,et al.  Morphological Traits Associated with the Quantitative Trait Locus for Lodging Tolerance in Soybean , 2019, Crop Science.

[3]  A. Tardivel,et al.  Comprehensive description of genomewide nucleotide and structural variation in short‐season soya bean , 2017, Plant biotechnology journal.

[4]  Arvind Kumar,et al.  Molecular Mapping of QTLs Associated with Lodging Resistance in Dry Direct-Seeded Rice (Oryza sativa L.) , 2017, Front. Plant Sci..

[5]  L. Qiu,et al.  Selection of soybean elite cultivars based on phenotypic and genomic characters related to lodging tolerance , 2017 .

[6]  Dong Cao,et al.  Natural variation at the soybean J locus improves adaptation to the tropics and enhances yield , 2017, Nature Genetics.

[7]  Z. Shan,et al.  Combining QTL and candidate gene analysis with phenotypic model to unravel the relationship between lodging and related traits in soybean , 2017, Molecular Breeding.

[8]  Ashkan Golshani,et al.  Mapping and identification of a potential candidate gene for a novel maturity locus, E10, in soybean , 2017, Theoretical and Applied Genetics.

[9]  Z. Zhang,et al.  Genetic architecture of lodging resistance revealed by genome- wide association study in maize (Zea mays L) , 2016 .

[10]  B. Ma,et al.  A new method for assessing plant lodging and the impact of management options on lodging in canola crop production , 2016, Scientific Reports.

[11]  Istvan Rajcan,et al.  Identification of loci governing eight agronomic traits using a GBS-GWAS approach and validation by QTL mapping in soya bean. , 2015, Plant biotechnology journal.

[12]  M. Ishimoto,et al.  Quantitative trait loci associated with lodging tolerance in soybean cultivar ‘Toyoharuka’ , 2014, Breeding science.

[13]  Chad D. Lee,et al.  Genetic Improvement of U.S. Soybean in Maturity Groups II, III, and IV , 2014 .

[14]  H. Kanamori,et al.  Natural variation in the genes responsible for maturity loci E1, E2, E3 and E4 in soybean. , 2014, Annals of botany.

[15]  K. Harada,et al.  Genetic and molecular bases of photoperiod responses of flowering in soybean , 2012, Breeding science.

[16]  Rong Zhou,et al.  Quantitative trait loci analysis of stem strength and related traits in soybean , 2011, Euphytica.

[17]  J. Specht,et al.  Artificial selection for determinate growth habit in soybean , 2010, Proceedings of the National Academy of Sciences.

[18]  Baohui Liu,et al.  The Soybean Stem Growth Habit Gene Dt1 Is an Ortholog of Arabidopsis TERMINAL FLOWER11[W][OA] , 2010, Plant Physiology.

[19]  Edward S. Buckler,et al.  TASSEL: software for association mapping of complex traits in diverse samples , 2007, Bioinform..

[20]  H. Cai,et al.  QTL analysis of lodging resistance and related traits in Italian ryegrass (Lolium multiflorum Lam.) , 2004, Theoretical and Applied Genetics.

[21]  S. Shimada,et al.  Evaluation of Root Lodging Resistance in Soybean (Glycine max(L.) Merr.) Cultivars by Measurement of Pushing Resistance and Top Weight Moment , 2002 .

[22]  J. Wilcox.  Sixty Years of Improvement in Publicly Developed Elite Soybean Lines , 2001 .

[23]  M. Morrison,et al.  Agronomic changes from 58 years of genetic improvement of short-season soybean cultivars in Canada , 1999 .

[24]  Elroy R. Cober,et al.  Fifty‐Eight Years of Genetic Improvement of Short‐Season Soybean Cultivars in Canada , 1997 .

[25]  E. V. Laureles,et al.  Lodging reduces yield of rice by self-shading and reductions in canopy photosynthesis , 1997 .

[26]  D. Ashley,et al.  Molecular Markers Associated with Soybean Plant Height, Lodging, and Maturity across Locations , 1996 .

[27]  D. Ashley,et al.  Identification of quantitative trait loci for plant height, lodging, and maturity in a soybean population segregating for growth habit , 1996, Theoretical and Applied Genetics.

[28]  Sylvain Pellerin,et al.  A portable electronic device for evaluating root lodging resistance in maize , 1995 .

[29]  S. Akita,et al.  Eco-Physiological Characteristics Related with Lodging Tolerance of Rice in Direct Sowing Cultivation : II. Root growth characteristics of tolerant cultivars to root lodging , 1994 .

[30]  S. Akita,et al.  Eco-Physiological Characteristics Related with Lodging Tolerance of Rice in Direct Sowing Cultivation : I. Comparison of the root lodging tolerance among cultivars by the measurement of pushing resistance , 1992 .

[31]  R. L. Bernard,et al.  Genetic Improvement of Soybeans in the Midwest1 , 1979 .

[32]  V. D. Luedders.  Genetic Improvement in Yield of Soybeans1 , 1977 .

[33]  M. Idris,et al.  The effect of silicon on lodging of rice in presence of added nitrogen , 1975, Plant and Soil.

[34]  R. Cooper.  Influence of Early Lodging on Yield of Soybean [Glycine max (L.) Merr.]1 , 1971 .

[35]  W. Schapaugh,et al.  Changes in Physiological Traits in Soybean with Breeding Advancements , 2016 .

[36]  Pooja Murlidharan,et al.  Genetic progress of soybean varieties released during 1969 to 2008 in India , 2011 .

[37]  W. Davies,et al.  Raising yield potential of wheat. III. Optimizing partitioning to grain while maintaining lodging resistance. , 2011, Journal of experimental botany.

[38]  Huijun Guo,et al.  Quantitative trait loci (QTL) of stem strength and related traits in a doubled-haploid population of wheat (Triticum aestivum L.) , 2005, Euphytica.

[39]  Lon R. Cardon,et al.  Quantitative Trait Loci , 1995 .