Diffuse Reflectance Spectroscopy as a Tool to Measure the Absorption Coefficient in Skin: South African Skin Phototypes

In any laser skin treatment, the optical properties (absorption and scattering coefficients) are important parameters. The melanin content of skin influences the absorption of light in the skin. The spread in the values of the absorption coefficients for the South African skin phototypes are not known. A diffuse reflectance probe consisting of a ring of six light delivery fibers and a central collecting fiber was used to measure the diffused reflected light from the arms of 30 volunteers with skin phototypes I–V (on the Fitzpatrick scale). The absorption coefficient was calculated from these measurements. This real‐time in vivo technique was used to determine the absorption coefficient of sun‐exposed and ‐protected areas on the arm. The range of typical absorption coefficients for the South African skin phototypes is reported. The values for the darker South African skin types were much higher than was previously reported for darker skin phototypes. In the analysis, the contributions of the eumelanin and pheomelanin were separated, which resulted in improved curve fitting for volunteers of southern Asian ethnicity without compromising the other groups.

[1]  Moritz Stoerring,et al.  Computer vision and human skin colour , 2004 .

[2]  Anthony J. Durkin,et al.  Chromophore concentrations, absorption and scattering properties of human skin in-vivo. , 2009, Optics express.

[3]  J Wu,et al.  Diffuse reflectance from turbid media: an analytical model of photon migration. , 1993, Applied optics.

[4]  George Zonios,et al.  Modeling diffuse reflectance from semi-infinite turbid media: application to the study of skin optical properties. , 2006, Optics express.

[5]  R. Doornbos,et al.  The determination of in vivo human tissue optical properties and absolute chromophore concentrations using spatially resolved steady-state diffuse reflectance spectroscopy. , 1999, Physics in medicine and biology.

[6]  E. Battle,et al.  Laser‐assisted hair removal for darker skin types , 2004, Dermatologic therapy.

[7]  T. Fitzpatrick The validity and practicality of sun-reactive skin types I through VI. , 1988, Archives of Dermatology.

[8]  Renato Marchesini,et al.  In vivo characterization of melanin in melanocytic lesions: spectroscopic study on 1671 pigmented skin lesions. , 2009, Journal of biomedical optics.

[9]  G. Zonios,et al.  Skin melanin, hemoglobin, and light scattering properties can be quantitatively assessed in vivo using diffuse reflectance spectroscopy. , 2001, The Journal of investigative dermatology.

[10]  Experimental verification and validation of a computer model for light–tissue interaction , 2011, Lasers in Medical Science.

[11]  S. Alaluf,et al.  Ethnic variation in melanin content and composition in photoexposed and photoprotected human skin. , 2002, Pigment cell research.

[12]  I. V. Meglinsky,et al.  Modelling the sampling volume for skin blood oxygenation measurements , 2006, Medical and Biological Engineering and Computing.

[13]  George Zonios,et al.  Melanin optical properties provide evidence for chemical and structural disorder in vivo. , 2008, Optics express.

[14]  B. Moncada,et al.  Diffuse reflectance spectrophotometry for skin phototype determination , 2010, Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging.

[15]  I. Meglinski,et al.  Quantitative assessment of skin layers absorption and skin reflectance spectra simulation in the visible and near-infrared spectral regions. , 2002, Physiological measurement.

[16]  Hanli Liu,et al.  Determination of reduced scattering coefficient of biological tissue from a needle-like probe. , 2005, Optics express.

[17]  M. Kohl,et al.  Near-infrared optical properties of ex vivo human skin and subcutaneous tissues measured using the Monte Carlo inversion technique. , 1998, Physics in medicine and biology.

[18]  V. Tuchin Tissue Optics: Light Scattering Methods and Instruments for Medical Diagnosis , 2000 .

[19]  M. Braun,et al.  Diffuse reflectance spectroscopy as a tool to measure the absorption coefficient in skin: system calibration , 2013, Lasers in Medical Science.

[20]  A. N. Bashkatov,et al.  Optical properties of human skin, subcutaneous and mucous tissues in the wavelength range from 400 to 2000 nm , 2005 .

[21]  N Kollias,et al.  The physical basis of skin color and its evaluation. , 1995, Clinics in dermatology.

[22]  Roberto Reif,et al.  Analytical model of light reflectance for extraction of the optical properties in small volumes of turbid media. , 2007, Applied optics.

[23]  S. Lanigan,et al.  Incidence of side effects after laser hair removal. , 2003, Journal of the American Academy of Dermatology.