Chemical germination of native and cation-exchanged bacterial spores with trifluoperazine

The calmodulin antagonist trifluoperazine and its analog chlorpromazine, both amphipaths, induced chemical germination of spores of various species, as do many surfactants. Cation load can greatly influence this response. Calmodulin antagonism does not seem to be involved. A new fluorometric assay for dipicolinic acid based on the fluorescence of the dipicolinic acid chelate of Tb3+ was found to be simple and sensitive.

[1]  S. Giunta,et al.  Chlorpromazine as permeabilizer and reagent for detection of microbial peroxidase and peroxidaselike activities , 1986, Applied and environmental microbiology.

[2]  M. Lieber,et al.  Interaction of chlorpromazine with the human erythrocyte membrane. , 1984, The Journal of biological chemistry.

[3]  H. Halvorson,et al.  Trypsinlike enzymes from dormant and germinated spores of Bacillus cereus T and their possible involvement in germination , 1985, Journal of bacteriology.

[4]  D. Papahadjopoulos,et al.  Studies on the mechanism of membrane fusion: kinetics of calcium ion induced fusion of phosphatidylserine vesicles followed by a new assay for mixing of aqueous vesicle contents. , 1980, Biochemistry.

[5]  W. Crocker.  Germination , 1915, Botanical Gazette.

[6]  J. Vary,et al.  Effect of ion channel blockers on germination of Bacillus megaterium spores , 1986 .

[7]  P. M. Foegeding,et al.  Presence of calmodulin-like calcium-binding protein in Bacillus cereus T spores , 1989 .

[8]  J. Foster,et al.  Influence of Exchangeable Ions on Germinability of Bacterial Spores , 1966, Journal of bacteriology.

[9]  F. W. Janssen,et al.  Colorimetric assay for dipicolinic acid in bacterial spores. , 1958, Science.

[10]  J. Foster,et al.  Endotrophic Calcium, Strontium, and Barium Spores of Bacillus megaterium and Bacillus cereus , 1966, Journal of bacteriology.

[11]  L. E. Sacks.  Modified two-phase system for partition of Bacillus macerans spores. , 1969, Applied microbiology.

[12]  P. M. Foegeding,et al.  Involvement of the spore coat in germination of Bacillus cereus T spores , 1987, Applied and environmental microbiology.

[13]  P. Seeman,et al.  The membrane actions of anesthetics and tranquilizers. , 1972, Pharmacological reviews.

[14]  L. E. Sacks,et al.  BEHAVIOR OF BACTERIAL SPORES IN AQUEOUS POLYMER TWO-PHASE SYSTEMS , 1961, Journal of bacteriology.

[15]  A. Sherry,et al.  A simple, one-step fluorometric method for determination of nanomolar concentrations of terbium. , 1976, Analytical biochemistry.

[16]  N. Snell,et al.  Base exchange and heat resistance in bacterial spores. , 1963, Biochemical and biophysical research communications.

[17]  D. Ellar,et al.  Study of calcium dipicolinate release during bacterial spore germination by using a new, sensitive assay for dipicolinate , 1978, Journal of bacteriology.

[18]  R. Lundin,et al.  High-resolution solid-state 13C nuclear magnetic resonance of bacterial spores: identification of the alpha-carbon signal of dipicolinic acid , 1988, Applied and environmental microbiology.

[19]  P. Thompson,et al.  Germination Requirements of Bacillus macerans Spores , 1971, Journal of bacteriology.

[20]  G. Kuehn,et al.  Calmodulin-like protein from Bacillus subtilis. , 1986, Biochemical and biophysical research communications.

[21]  H. Hauser,et al.  The displacement of calcium ions from phospholipid monolayers by pharmacologically active and other organic bases. , 1968, The Biochemical journal.

[22]  J. Foster,et al.  GERMINATION OF BACTERIAL SPORES WITH ALKYL PRIMARY AMINES , 1961, Journal of bacteriology.

[23]  G. Bender,et al.  Spore heat resistance and specific mineralization , 1985, Applied and environmental microbiology.