Effect of Benzaldehyde on Survivals of Chinese Hamster V-79 Cells in Vitro from The Combined Treatment with Hyperthermia

(1) The effects of benzaldehyde (BA) in the slightly cytotoxic concentration on cell survivalsfrom hyperthermia as observed in colony formation representing markedly enhanced thermosensitivity of Chinese hamster V-79 cells in vitro. (2) After incubation of benzylidene glucopyranose (BG) under condition of pH 3 for 22 hr, half an amount of BG was hydrolysed to BA as observed with the specific absorbance at 250 nm of BA. (3) BA is a hydrolysate of amygdaline that enhanced thermosensitivity. Other hand BG did not show any appreciable interaction for thermo-enhancement but showed no more than the additive effect. Adopted concentration of BA or BG was 1.8 mM. BG in 1.8mM was treated under pH 3 for 22 hr, resulting in half a BG was hydrolysed. It was designated BG*. (4) Indication of BA or its derivatives would be possible thermo-enhancer in human clinical interdisciplinary oncotherapy including hyperthermia.