论文信息 - Simultaneous measurement of sensor-protein dynamics and motility of a single cell by on-chip microcultivation system

Simultaneous measurement of sensor-protein dynamics and motility of a single cell by on-chip microcultivation system

Measurement of the correlation between sensor-protein expression, motility and environmental change is important for understanding the adaptation process of cells during their change of generation. We have developed a novel assay exploiting the on-chip cultivation system, which enabled us to observe the change of the localization of expressed sensor-protein and the motility for generations. Localization of the aspartate sensitive sensor protein at two poles in Escherichia coli decreased quickly after the aspartate was added into the cultivation medium. However, it took more than three generations for recovering the localization after the removal of aspartate from the medium. Moreover, the tumbling frequency was strongly related to the localization of the sensor protein in a cell. The results indicate that the change of the spatial localization of sensor protein, which was inherited for more than three generations, may contribute to cells, motility as the inheritable information.

[1] J. Stock,et al. Stimulus response coupling in bacterial chemotaxis: receptor dimers in signalling arrays , 1998, Molecular microbiology.

[2] Judith P. Armitage,et al. Bacterial Locomotion and Signal Transduction , 1998, Journal of bacteriology.

[3] Kenji Yasuda,et al. On-chip single-cell microcultivation assay for monitoring environmental effects on isolated cells. , 2003, Biochemical and biophysical research communications.

[4] J. Maddock,et al. Polar Clustering of the Chemoreceptor Complex inEscherichia coli Occurs in the Absence of Complete CheA Function , 2000, Journal of bacteriology.

[5] Hiroyuki Moriguchi,et al. Analysis of single-cell differences by use of an on-chip microculture system and optical trapping , 2001, Fresenius' journal of analytical chemistry.

[6] Robert Mesibov,et al. Chemotaxis Toward Amino Acids in Escherichia coli , 1972, Journal of bacteriology.

[7] L. Shapiro,et al. Polar location of the chemoreceptor complex in the Escherichia coli cell. , 1993, Science.

[8] Y Wakamoto,et al. On-chip culture system for observation of isolated individual cells. , 2001, Lab on a chip.

[9] J. Maddock,et al. Clustering of the Chemoreceptor Complex inEscherichia coli Is Independent of the Methyltransferase CheR and the Methylesterase CheB , 1999, Journal of bacteriology.