In VivoExpression of Insulin-Like Growth Factor-Binding Protein-2 in Human Gliomas Increases with the Tumor Grade.

Human central nervous system tumors and glioma cell lines highly express the insulin-like growth factor-binding protein (IGFBP)-2. As IGFBP-2 can affect tumor growth, we studied the relationship between IGFBP-2 expression and the malignancy of brain tumors in vivo. To do so, we investigated by immunohistochemistry the accumulation of IGFBP-1, -2, and -3 in 50 human gliomas classified by the WHO Malignancy Scale. Double labeling using anti-CD68 (monocytes/macrophages), antiglial fibrillary acidic protein, and anti-CD3 (T cells) antibodies was performed to further characterize the IGFBP-1, -2, and -3+ cells. The expression of IGFBP messenger RNAs (mRNAs) was tested by RT-PCR in tumor samples from nine gliomas of different grades and in eight cell lines representing the cellular composition of human glioma. As controls, the accumulation of IGFBP-2 was investigated in normal brain and in the rat C6 glioblastoma model. IGFBP-1 and -3 accumulated in endothelial and macrophage/microglial cells. IGFBP-2+ macropha...

[1]  M. Boes,et al.  Regulation of endothelial IGFBP-3 synthesis and secretion by IGF-I and TGF-beta. , 1996, Growth regulation.

[2]  S. Bowen,et al.  Differential IGF‐independent effects of insulin‐like growth factor binding proteins (1–6) on apoptosis of breast epithelial cells , 1999, Journal of cellular biochemistry.

[3]  T. Unterman,et al.  Production of insulin-like growth factor-binding proteins by human central nervous system tumors. , 1991, Cancer research.

[4]  M. Elmlinger,et al.  Elevated insulin-like growth factor (IGF) binding protein (IGFBP)-2 and IGFBP-4 expression of leukemic T-cells is affected by autocrine/paracrine IGF-II action but not by IGF type I receptor expression. , 1998, European journal of endocrinology.

[5]  H. von Holst,et al.  Increase of insulin-like growth factor (IGF)-1, IGF binding protein-2 and -4 mRNAs following cerebral contusion. , 1996, Brain research. Molecular brain research.

[6]  M. Weller,et al.  Patterns of cyclooxygenase-1 and -2 expression in human gliomas in vivo , 1999, Acta Neuropathologica.

[7]  E. Feldman,et al.  Immunohistochemical localization of insulin-like growth factor-II (IGF-II) and IGF-binding protein-2 during development in the rat brain. , 1994, Endocrinology.

[8]  D. Yee,et al.  Activation of estrogen receptor-mediated gene transcription by IGF-I in human breast cancer cells. , 1997, The Journal of endocrinology.

[9]  M. Resnicoff.  Antitumor effects elicited by antisense-mediated downregulation of the insulin-like growth factor I receptor (review). , 1998, International journal of molecular medicine.

[10]  K. Nackaerts,et al.  Heparan sulfate proteoglycans. Essential co-factors in receptor-mediated processes with relevance to the biology of the vascular wall. , 1995, Atherosclerosis.

[11]  P. Gluckman,et al.  Hypoxia and hypoxia/ischemia affect the expression of insulin-like growth factor binding protein 2 in the developing rat brain. , 1993, Brain research. Molecular brain research.

[12]  M. Tykocinski,et al.  Treatment and prevention of rat glioblastoma by immunogenic C6 cells expressing antisense insulin-like growth factor I RNA. , 1993, Science.

[13]  R. Meyermann,et al.  Multiple epitope labeling by the exclusive use of alkaline phosphatase conjugates in immunohistochemistry , 1998, Histochemistry and Cell Biology.

[14]  G. Seidel,et al.  Characterization of insulin-like growth factor-binding proteins in the uterus and conceptus during early conceptus elongation in cattle. , 1998, Biology of reproduction.

[15]  G. Werther,et al.  The Role of the Insulin-Like Growth Factor System in the Developing Brain , 1998, Hormone Research in Paediatrics.

[16]  B. Scheithauer,et al.  The New WHO Classification of Brain Tumours , 1993, Brain pathology.

[17]  D. Yee,et al.  Proliferation of cultured human prostate cancer cells is inhibited by insulin-like growth factor (IGF) binding protein-1: evidence for an IGF-II autocrine growth loop. , 1995, The Journal of clinical endocrinology and metabolism.

[18]  G. Kreutzberg,et al.  Expression of Insulin-like Growth Factor-I and Related Peptides during Motoneuron Regeneration , 1994, Experimental Neurology.

[19]  R. Atkins,et al.  A simple, reliable, and sensitive method for nonradioactive in situ hybridization: use of microwave heating to improve hybridization efficiency and preserve tissue morphology. , 1996, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[20]  V. Han,et al.  Overexpression of insulin-like growth factor-binding protein-2 in C6 glioma cells results in conditional alteration of cellular growth. , 1999, Endocrinology.

[21]  V. P. Collins,et al.  A high ratio of insulin-like growth factor II/insulin-like growth factor binding protein 2 messenger RNA as a marker for anaplasia in meningiomas. , 1997, Cancer research.

[22]  M. Pollak,et al.  Antiproliferative action of tumor necrosis factor-alpha on MCF-7 breastcancer cells is associated with increased insulin-like growth factor binding protein-3 accumulation. , 1998, International journal of oncology.

[23]  T. Roszman,et al.  Insulin-like growth factors (IGF) enhance three-dimensional (3D) growth of human glioblastomas. , 1997, Cancer letters.

[24]  G. Romano,et al.  Inhibition of tumorigenesis and induction of apoptosis in human tumor cells by the stable expression of a myristylated COOH terminus of the insulin-like growth factor I receptor. , 1998, Cancer research.

[25]  E. Diamandis,et al.  Insulin‐like growth factor–binding protein‐3 and breast cancer survival , 1998, International journal of cancer.

[26]  C. Azcona,et al.  Growth Hormone Treatment of Russell-Silver Syndrome , 1998, Hormone Research in Paediatrics.

[27]  G. Werther,et al.  Localization of mRNAs for insulin-like growth factor-I (IGF-I), IGF-I receptor, and IGF binding proteins in rat eye. , 1996, Investigative ophthalmology & visual science.

[28]  J. Dichgans,et al.  Predicting chemoresistance in human malignant glioma cells: The role of molecular genetic analyses , 1998, International journal of cancer.

[29]  R. Glazer,et al.  Induction of p53-dependent, insulin-like growth factor-binding protein-3-mediated apoptosis in glioblastoma multiforme cells by a protein kinase Calpha antisense oligonucleotide. , 1999, Molecular pharmacology.

[30]  W. Yung,et al.  Reactivation of insulin-like growth factor binding protein 2 expression in glioblastoma multiforme: a revelation by parallel gene expression profiling. , 1999, Cancer research.

[31]  R. Baserga,et al.  Regression of C6 rat brain tumors by cells expressing an antisense insulin-like growth factor I receptor RNA. , 1996, Journal of experimental therapeutics & oncology.

[32]  H. Schluesener,et al.  Cyclooxygenases-1 and -2 are differentially localized to microglia and endothelium in rat EAE and glioma , 1999, Journal of Neuroimmunology.

[33]  M. Elmlinger,et al.  Functional role of insulin-like growth factor binding proteins. , 1997, Hormone research.

[34]  R. Baserga,et al.  IGFs and Cell Growth , 1999 .

[35]  W. Blum,et al.  Insulin-like growth factor-binding protein-2 concentrations in cerebrospinal fluid and serum of children with malignant solid tumors or acute leukemia. , 1994, The Journal of clinical endocrinology and metabolism.

[36]  E. Anggard,et al.  Insulin-like growth factor binding protein-1 inhibits arterial smooth muscle cell proliferation in vitro but does not reduce the neointimal response to balloon catheter injury. , 1995, Atherosclerosis.