Determination of saffron (Crocus sativus L.) components in crude plant extract using high-performance liquid chromatography-UV-visible photodiode-array detection-mass spectrometry.

The determination of saffron components in crude plant extracts by high-performance liquid chromatography-UV-visible photodiode-array detection on-line with mass spectrometry is described. The method is shown to be suitable for the determination of picrocrocin, the glycosidic precursor of safranal, safranal and flavonoids; it is the technique of choice for the analysis of crocetin glycosides (crocins) carrying one up to five glucoses and differentiation of their trans and cis isomers.

[1]  M. Manfait,et al.  Separation of picrocrocin, cis-trans-crocins and safranal of saffron using high-performance liquid chromatography with photodiode-array detection. , 1994, Journal of chromatography. A.

[2]  M. Manfait,et al.  The structure of dimethylcrocetin , 1994 .

[3]  R. Kuhn,et al.  Über die Konstitution des Pikro‐crocins und seine Beziehung zu den Carotin‐Farbstoffen des Safrans , 1934 .

[4]  R. Macrae.  HPLC in food analysis. , 1982 .

[5]  W. Stahl,et al.  Separation of beta-carotene and lycopene geometrical isomers in biological samples. , 1993, Clinical chemistry.

[6]  P. Gramatica,et al.  13-cis-Crocin: A new crocinoid of saffron. , 1984 .

[7]  J. Vincken,et al.  Electrospray mass spectrometry of neutral and acidic oligosaccharides: Methylated cyclodextrins and identification of unknowns derived from fruit material , 1993 .

[8]  D. Pilbeam.  The Flavonoids: Advances in Research : Edited by J.B. Harborne and T.J. Mabry Chapman and Hall; London, New York, 1982 744 pages. £ 49.50 , 1983 .

[9]  S. Schwartz,et al.  Fast-atom bombardment and continuous-flow fast-atom bombardment mass spectrometry in carotenoid analysis , 1992 .

[10]  G. A. Ravishankar,et al.  Methods for the analysis of the saffron metabolites crocin, crocetins, picrocrocin and safranal for the determination of the quality of the spice using thin-layer chromatography, high-performance liquid chromatography and gas chromatography , 1992 .

[11]  H. Pfander,et al.  Separation of crocetin glycosyl esters by high-performance liquid chromatography , 1982 .

[12]  M. Maillard,et al.  Liquid chromatographic—thermospray mass spectrometric analysis of crude plant extracts containing phenolic and terpene glycosides , 1993 .

[13]  S. Schwartz,et al.  Chromatographic analysis of cis/trans carotenoid isomers. , 1992, Journal of chromatography.

[14]  M. Cánovas,et al.  TLC preparative purification of picrocrocin, HTCC and crocin from saffron , 1992 .

[15]  D. E. Heinz,et al.  Monoterpene aldehydes and isophorone-related compounds of saffron , 1971 .

[16]  R. Macrae.  HPLC in food analysis. 2nd ed. , 1988 .

[17]  M. Careri,et al.  Particle beam liquid chromatography—mass spectrometry behaviour of polynuclear metal carbonyl compounds , 1993 .

[18]  Wolfgang Rödel,et al.  Analysis of the volatile components of saffron , 1991 .

[19]  A. Burlingame,et al.  Desorption mass spectrometry of oligosaccharides coupled with hydrophobic chromophores. , 1990, Methods in enzymology.

[20]  J. Iborra,et al.  Preparative high-performance liquid chromatographic purification of saffron secondary metabolites , 1993 .

[21]  Continuous-flow fast-atom-bombardment liquid chromatography/mass spectrometry of carotenoids , 1993 .