Mussel-Inspired Electrospun Nanofibers Functionalized with Size-Controlled Silver Nanoparticles for Wound Dressing Application.

Electrospun nanofibers that contain silver nanoparticles (AgNPs) have a strong antibacterial activity that is beneficial to wound healing. However, most of the literature available on the bactericidal effects of this material is based on the use of AgNPs with uncontrolled size, shape, surface properties, and degree of aggregation. In this study, we report the first versatile synthesis of novel catechol moieties presenting electrospun nanofibers functionalized with AgNPs through catechol redox chemistry. The synthetic strategy allows control of the size and amount of AgNPs on the surface of nanofibers with the minimum degree of aggregation. We also evaluated the rate of release of the AgNPs, the biocompatibility of the nanofibers, the antibacterial activity in vitro, and the wound healing capacity in vivo. Our results suggest that these silver-releasing nanofibers have great potential for use in wound healing applications.

[1]  Myung-Seob Khil,et al.  Electrospun nanofibrous polyurethane membrane as wound dressing. , 2003, Journal of biomedical materials research. Part B, Applied biomaterials.

[2]  Renliang Huang,et al.  Facile in situ synthesis of silver nanoparticles on procyanidin-grafted eggshell membrane and their catalytic properties. , 2014, ACS applied materials & interfaces.

[3]  Haeshin Lee,et al.  Mussel-Inspired Surface Chemistry for Multifunctional Coatings , 2007, Science.

[4]  Cheol-Sang Kim,et al.  Electrospun antibacterial polyurethane-cellulose acetate-zein composite mats for wound dressing. , 2014, Carbohydrate polymers.

[5]  Xiaole Zhang,et al.  One-step synthesis of silver/dopamine nanoparticles and visual detection of melamine in raw milk. , 2011, The Analyst.

[6]  Olivera Stojadinovic,et al.  PERSPECTIVE ARTICLE: Growth factors and cytokines in wound healing , 2008, Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society.

[7]  J. Jang,et al.  Synthesis and antimicrobial properties of novel silver/polyrhodanine nanofibers. , 2008, Biomacromolecules.

[8]  Ho Yeon Son,et al.  Silver‐Polydopamine Hybrid Coatings of Electrospun Poly(vinyl alcohol) Nanofibers , 2013 .

[9]  F. Stadler,et al.  Mussel-inspired pH-triggered reversible foamed multi-responsive gel--the surprising effect of water. , 2013, Chemical communications.

[10]  T. Phan,et al.  Fabrication and Characterization of Nanostructured and Thermosensitive Polymer Membranes for Wound Healing and Cell Grafting , 2006 .

[11]  Juan Fan,et al.  Acceleration of dermal wound healing by using electrospun curcumin-loaded poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) fibrous mats. , 2014, Journal of biomedical materials research. Part B, Applied biomaterials.

[12]  E. Caterson,et al.  Tissue engineering of skin. , 2013, Journal of the American College of Surgeons.

[13]  P. Messersmith,et al.  Bacterial killing by light-triggered release of silver from biomimetic metal nanorods. , 2014, Small.

[14]  Ahmed I. Abdelrahman,et al.  On the aggregation phenomena of Au nanoparticles: Effect of substrate roughness on the particle size , 2008 .

[15]  J. Ji,et al.  Electropolymerization of dopamine for surface modification of complex-shaped cardiovascular stents. , 2014, Biomaterials.

[16]  K. Landfester,et al.  Antibacterial Surface Coatings from Zinc Oxide Nanoparticles Embedded in Poly(N‐isopropylacrylamide) Hydrogel Surface Layers , 2012 .

[17]  Yuhan Lee,et al.  Facile fabrication of branched gold nanoparticles by reductive hydroxyphenol derivatives. , 2011, Langmuir : the ACS journal of surfaces and colloids.

[18]  Won Jong Kim,et al.  Poly(norepinephrine): ultrasmooth material-independent surface chemistry and nanodepot for nitric oxide. , 2013, Angewandte Chemie.

[19]  P. Supaphol,et al.  Wound-dressing materials with antibacterial activity from electrospun gelatin fiber mats containing silver nanoparticles , 2008 .

[20]  Min Soo Bae,et al.  Electrospun chitosan nanofibers with controlled levels of silver nanoparticles. Preparation, characterization and antibacterial activity. , 2014, Carbohydrate polymers.

[21]  M. Valiente,et al.  Poly(N-isopropylacrylamide)/Gold Hybrid Hydrogels Prepared by Catechol Redox Chemistry. Characterization and Smart Tunable Catalytic Activity , 2014 .

[22]  Darrin J Pochan,et al.  Synthesis and antibacterial properties of silver nanoparticles. , 2005, Journal of nanoscience and nanotechnology.

[23]  Hak Yong Kim,et al.  Wound-dressing materials with antibacterial activity from electrospun polyurethane-dextran nanofiber mats containing ciprofloxacin HCl. , 2012, Carbohydrate polymers.

[24]  T. A. Hatton,et al.  Electrospun magnetic carbon composite fibers: Synthesis and electromagnetic wave absorption characteristics , 2013 .

[25]  Jessica D. Schiffman,et al.  Designing electrospun nanofiber mats to promote wound healing - a review. , 2013, Journal of materials chemistry. B.

[26]  Bishara S Atiyeh,et al.  Effect of silver on burn wound infection control and healing: review of the literature. , 2007, Burns : journal of the International Society for Burn Injuries.

[27]  Haeshin Lee,et al.  General functionalization route for cell adhesion on non-wetting surfaces. , 2010, Biomaterials.

[28]  Sook Hee Ku,et al.  Human endothelial cell growth on mussel-inspired nanofiber scaffold for vascular tissue engineering. , 2010, Biomaterials.

[29]  José G Rivera,et al.  Mussel-inspired silver-releasing antibacterial hydrogels. , 2012, Biomaterials.

[30]  L. Lagae,et al.  Magnetic Electrospun Fibers for Cancer Therapy , 2012 .

[31]  F. Stadler,et al.  Mussel‐Inspired Electrospun Smart Magnetic Nanofibers for Hyperthermic Chemotherapy , 2015 .

[32]  D.Q. Zhao,et al.  Silver nanoparticle/chitosan oligosaccharide/poly(vinyl alcohol) nanofibers as wound dressings: a preclinical study , 2013, International journal of nanomedicine.

[33]  P. Opanasopit,et al.  Electrospun chitosan/polyvinyl alcohol nanofibre mats for wound healing , 2014, International wound journal.

[34]  Noorsaiyyidah Darman Singho,et al.  FTIR Studies on Silver-Poly(Methylmethacrylate) Nanocomposites via In-Situ Polymerization Technique , 2012 .

[35]  J. Jang,et al.  Aqueous synthesis of silver nanoparticle embedded cationic polymer nanofibers and their antibacterial activity. , 2012, ACS applied materials & interfaces.

[36]  M. Bruening,et al.  Development of polymeric hollow fiber membranes containing catalytic metal nanoparticles , 2010 .

[37]  Milan Kolar,et al.  Silver colloid nanoparticles: synthesis, characterization, and their antibacterial activity. , 2006, The journal of physical chemistry. B.

[38]  C. Hauser,et al.  In situ synthesis of size-controlled, stable silver nanoparticles within ultrashort peptide hydrogels and their anti-bacterial properties. , 2014, Biomaterials.

[39]  S. Werner,et al.  Wound repair and regeneration , 1994, Nature.

[40]  D. Shen,et al.  Polydopamine-coated nanofibrous mats as a versatile platform for producing porous functional membranes , 2012 .

[41]  Ziwei Deng,et al.  Mussel-inspired polydopamine coating as a versatile platform for synthesizing polystyrene/Ag nanocomposite particles with enhanced antibacterial activities. , 2014, Journal of materials chemistry. B.

[42]  M. Bruening,et al.  Catalytic hollow fiber membranes prepared using layer-by-layer adsorption of polyelectrolytes and metal nanoparticles , 2010 .

[43]  C. Murphy,et al.  Polymeric Multilayers that Contain Silver Nanoparticles can be Stamped onto Biological Tissues to Provide Antibacterial Activity , 2011, Advanced functional materials.

[44]  Florian J. Stadler,et al.  Rapid self-healing and triple stimuli responsiveness of a supramolecular polymer gel based on boron–catechol interactions in a novel water-soluble mussel-inspired copolymer , 2014 .

[45]  Tae Hwan Choi,et al.  Oxygen concentration control of dopamine-induced high uniformity surface coating chemistry. , 2013, ACS applied materials & interfaces.