Expansion and characterization of adipose tissue-derived stromal cells cultured with low serum medium.

Adipose tissue contains a population of cells that have extensive self-renewal capacity and the ability to differentiate along multiple lineages. In addition, adipose tissue-derived stromal cells (ATSCs) are able to differentiate into various cell types that may be useful for autologous cell transplantation for defects of bone, cartilage, adipose, and tendon, etc. Most protocols for in vitro cultures of ATSCs include fetal bovine serum (FBS) as a nutritional supplement. However, in some cell cultures, it involves multiple doses of FBS, which raises a concern over possible infections as well as immunological reactions that are caused by medium-derived FBS proteins, sialic acid, etc. In this study, we were able to expand mouse ATSCs using low mouse serum media containing collagen type I, heparin-carrying polystyrene, and fibroblast growth factor (FGF)-2. These expanded mouse ATSCs maintained their multilineage potential for differentiation into adipocytes, osteoblasts, and chondrocytes. Therefore, this method, which uses autologous cells and low serum media, may be able to be utilized for clinical cell therapies.

[1]  A. Schäffler,et al.  Concise Review: Adipose Tissue‐Derived Stromal Cells—Basic and Clinical Implications for Novel Cell‐Based Therapies , 2007, Stem cells.

[2]  Bonpei Takase,et al.  Tissue engineering of articular cartilage with autologous cultured adipose tissue-derived stromal cells using atelocollagen honeycomb-shaped scaffold with a membrane sealing in rabbits. , 2006, Journal of biomedical materials research. Part B, Applied biomaterials.

[3]  M. Ishihara,et al.  Establishment of a novel method for enriching osteoblast progenitors from adipose tissues using a difference in cell adhesive properties. , 2006, Biochemical and biophysical research communications.

[4]  Miya Ishihara,et al.  Bone formation using human adipose tissue-derived stromal cells and a biodegradable scaffold. , 2006, Journal of biomedical materials research. Part B, Applied biomaterials.

[5]  F. Gage,et al.  Human embryonic stem cells express an immunogenic nonhuman sialic acid , 2005, Nature Medicine.

[6]  M. Neubauer,et al.  Basic fibroblast growth factor enhances PPARγ ligand‐induced adipogenesis of mesenchymal stem cells , 2004, FEBS letters.

[7]  Miya Ishihara,et al.  Osteogenic Potential of Human Adipose Tissue-Derived Stromal Cells as an Alternative Stem Cell Source , 2004, Cells Tissues Organs.

[8]  T. Tsukazaki,et al.  Isolation and characterization of a mesenchymal cell line that differentiates into osteoblasts in response to BMP-2 from calvariae of GFP transgenic mice. , 2004, Bone.

[9]  Jianwei Wang,et al.  Growth factor regulation of human growth plate chondrocyte proliferation in vitro. , 2004, Biochemical and biophysical research communications.

[10]  Shu-Ching Hsu,et al.  Internalized antigens must be removed to prepare hypoimmunogenic mesenchymal stem cells for cell and gene therapy. , 2004, Molecular therapy : the journal of the American Society of Gene Therapy.

[11]  B. Larson,et al.  Adult stem cells from bone marrow (MSCs) isolated from different strains of inbred mice vary in surface epitopes, rates of proliferation, and differentiation potential. , 2004, Blood.

[12]  Min Zhu,et al.  Human adipose tissue is a source of multipotent stem cells. , 2002, Molecular biology of the cell.

[13]  Roy J Martin,et al.  The biology of white adipocyte proliferation , 2001, Obesity reviews : an official journal of the International Association for the Study of Obesity.

[14]  M. Ishihara,et al.  Heparin-carrying polystyrene (HCPS)-bound collagen substratum to immobilize heparin-binding growth factors and to enhance cellular growth. , 2001, Journal of biomedical materials research.

[15]  F. Barry,et al.  Chondrogenic differentiation of mesenchymal stem cells from bone marrow: differentiation-dependent gene expression of matrix components. , 2001, Experimental cell research.

[16]  M. Ishihara,et al.  Enhanced ability of heparin-carrying polystyrene (HCPS) to bind to heparin-binding growth factors and to inhibit growth factor-induced endothelial cell growth. , 2000, Journal of biochemistry.

[17]  M. Ishihara,et al.  Heparin-carrying polystyrene to mediate cellular attachment and growth via interaction with growth factors. , 2000, Journal of biomedical materials research.

[18]  M. Pittenger,et al.  Multilineage potential of adult human mesenchymal stem cells. , 1999, Science.

[19]  D. Adams,et al.  Modulation of cytokine function by heparan sulfate proteoglycans: sophisticated models for the regulation of cellular responses to cytokines. , 1998, Proceedings of the Association of American Physicians.

[20]  D. Prockop.  Marrow Stromal Cells as Stem Cells for Nonhematopoietic Tissues , 1997, Science.

[21]  R. Walker,et al.  Development of antibodies to fetal calf serum with arthus-like reactions in human immunodeficiency virus-infected patients given syngeneic lymphocyte infusions. , 1997, Blood.

[22]  R L Jackson,et al.  Glycosaminoglycans: molecular properties, protein interactions, and role in physiological processes. , 1991, Physiological reviews.

[23]  D. Gospodarowicz,et al.  Regulation of bovine bone cell proliferation by fibroblast growth factor and transforming growth factor beta. , 1988, Endocrinology.