The effect of injective application of selenopyran on the prolonged increase of the selenium content in blood and sperm of rams.

: Selenium is a microelement of big importance for male reproduction. As a part of the antioxidative enzyme gluthationperoxidase and structural Se proteins, it plays pivotal role in the defense of spermatozoa against generated ROS and in ensuring of its motility. During the last years the interests to the organic forms of selenium was enhanced because of its better biological utilization. The present work aimed to study the effect of injective application of organic compound selenopyran in rams on the distribution of selenium content in blood and sperm and on the changes in sperm quality. The experiment was conducted with 5 rams from the Synthetic Population Bulgarian Milk breed at the age between 3-7 years and live weight 85-90 kg. The animals were injected once with an oil solution of the selenopyran in dose of 0.1mg/kg live weight (selenium content 24%) 45 days before starting the breeding season. The blood was collected before treatment and 45 days thereafter. At the same time the first and second ejaculates of rams were collected using artificial vagina and analyzed by Sperm Class Analyzer. The selenium content was measured in plasma, blood cells and sperm by atomic absorption spectrometry using SpectrAA 55B double beam spectrometer (Varian, Inc.). The results showed that one injection of organic compound of selenium – selenopyran in dose of 0.1mg/kg live weight ensured the increase and support of high level of selenium in blood (plasma and blood cells) and in sperm during investigated period. That lead to the proper spermatogenesis in testis and allowed the production of qualitative ejaculates with higher number of total spermatozoa as well as of spermatozoa with progressive motility.