Plasmonic nanochip for SERS chemical and biomedical sensing

The development of rapid, easy-to-use and highly sensitive DNA detection methods has received increasing interest for medical diagnostics and research purposes. Our laboratory has developed several chip-based DNA biosensors including molecular sentinel-on-chip (MSC), multiplex MSC, and inverse molecular sentinel-on-chip (iMS-on-Chip). These sensors use surface-enhanced Raman scattering (SERS) plasmonic chips, functionalized with DNA probes for single-step DNA detection. The sensing mechanisms is based on the hybridization of target sequences and DNA probes, resulting in a displacement of a SERS reporter from the chip surface. This distance increase results in change in SERS signal intensity from the reporter, thus indicating the capture, and therefore the presence, of the target nucleic acid sequence. The nucleic acid probes and the SERS chip, which compose the sensing platform, were designed for single-step DNA detection. The target sequences are detected by delivery of a sample solutions on a functionalized chip and characterization of the SERS signals, after 1 - 2 hr incubation. These techniques avoid labeling of the target sequence or washing to remove unreacted components, making them easy-to-use and cost effective. The use of SERS chip for medical diagnostics was demonstrated by detecting genetic biomarkers for respiratory viral infection and the DNA of dengue virus 4.

[1]  Tuan Vo-Dinh,et al.  Surface-enhanced Raman spectrometry for trace organic analysis , 1984 .

[2]  Tuan Vo-Dinh,et al.  Multiplex detection of disease biomarkers using SERS molecular sentinel-on-chip , 2014, Analytical and Bioanalytical Chemistry.

[3]  Yang Liu,et al.  SERS nanosensors and nanoreporters: golden opportunities in biomedical applications. , 2015, Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology.

[4]  Tuan Vo-Dinh,et al.  Surface-enhanced Raman spectroscopy using metallic nanostructures , 1998 .

[5]  Tuan Vo-Dinh,et al.  Detection of human immunodeficiency virus type 1 DNA sequence using plasmonics nanoprobes. , 2005, Analytical chemistry.

[6]  L. Dick,et al.  Metal film over nanosphere (MFON) electrodes for surface-enhanced Raman spectroscopy (SERS): Improvements in surface nanostructure stability and suppression of irreversible loss , 2002 .

[7]  Tuan Vo-Dinh,et al.  Sensitive DNA detection and SNP discrimination using ultrabright SERS nanorattles and magnetic beads for malaria diagnostics. , 2016, Biosensors & bioelectronics.

[8]  Chit Yaw Fu,et al.  Enhancement in SERS intensity with hierarchical nanostructures by bimetallic deposition approach , 2012 .

[9]  Tuan Vo-Dinh,et al.  Label-free DNA biosensor based on SERS Molecular Sentinel on Nanowave chip. , 2013, Analytical chemistry.

[10]  Tuan Vo-Dinh,et al.  DNA bioassay-on-chip using SERS detection for dengue diagnosis. , 2014, The Analyst.

[11]  Tuan Vo-Dinh,et al.  Plasmonic SERS biosensing nanochips for DNA detection , 2016, Analytical and Bioanalytical Chemistry.

[12]  Tuan Vo-Dinh,et al.  Plasmonic Nanoparticles and Nanowires: Design, Fabrication and Application in Sensing. , 2010, The journal of physical chemistry. C, Nanomaterials and interfaces.

[13]  Christopher G. Khoury,et al.  Plasmonic nanoprobes: from chemical sensing to medical diagnostics and therapy. , 2013, Nanoscale.

[14]  Jean-Francois Masson,et al.  Surface-Enhanced Raman Spectroscopy Amplification with Film over Etched Nanospheres , 2010 .

[15]  Chad A Mirkin,et al.  Microfluidic-SERS devices for one shot limit-of-detection. , 2014, The Analyst.

[16]  Tuan Vo-Dinh,et al.  Plasmonic "Nanowave" Substrates for SERS: Fabrication and Numerical Analysis. , 2012, The journal of physical chemistry. C, Nanomaterials and interfaces.

[17]  Simion Astilean,et al.  Mapping the SERS Efficiency and Hot-Spots Localization on Gold Film over Nanospheres Substrates , 2010 .

[18]  Sumeet Mahajan,et al.  Reproducible SERRS from structured gold surfaces. , 2007, Physical chemistry chemical physics : PCCP.

[19]  Hyungsoon Im,et al.  Self‐Assembled Plasmonic Nanoring Cavity Arrays for SERS and LSPR Biosensing , 2013, Advanced materials.

[20]  Richard P Van Duyne,et al.  In vivo, transcutaneous glucose sensing using surface-enhanced spatially offset Raman spectroscopy: multiple rats, improved hypoglycemic accuracy, low incident power, and continuous monitoring for greater than 17 days. , 2011, Analytical chemistry.

[21]  Duncan Graham,et al.  Surface-Enhanced Raman Scattering (SERS) and Surface-Enhanced Resonance Raman Scattering (SERRS): A Review of Applications , 2011, Applied spectroscopy.