Quince seed mucilage coated iron oxide nanoparticles for plasmid DNA delivery

This study investigates the potential of iron oxide nanoparticles (Fe3O4) and quince seed mucilage as combined genetic carriers to deliver plasmid DNA (pDNA) through the gastrointestinal system. The samples are characterized by x-ray diffraction (XRD), zeta potential, dynamic light scattering, FT-IR spectroscopy, field emission scanning electron microscopy and vibrating sample magnetometry. The stability of pDNA loading on the nanocarriers and their release pattern are evaluated in simulated gastrointestinal environments by electrophoresis. The XRD patterns reveal that the nanocarriers could preserve their structure during various synthesis levels. The saturation magnetization (M s) of the Fe3O4 cores are 56.48 emu g−1 without any magnetic hysteresis. Not only does the loaded pDNA contents experience a remarkable stability in the simulated gastric environment, but also, they could be released up to 99% when exposed to an alkaline environment similar to the intestinal fluid of fish. The results indicate that the synthesized nanoparticles could be employed as efficient low-cost pDNA carriers.

[1]  V. Zeleňák,et al.  pH-responsive mesoporous silica drug delivery system, its biocompatibility and co-adsorption/co-release of 5-Fluorouracil and Naproxen , 2021 .

[2]  S. Khorasani,et al.  Facile preparation of chitosan-dopamine-inulin aldehyde hydrogel for drug delivery application. , 2021, International journal of biological macromolecules.

[3]  G. González-Aseguinolaza,et al.  Novel vectors and approaches for gene therapy in liver diseases , 2021, JHEP reports : innovation in hepatology.

[4]  Bradford J. Smith,et al.  Cerium oxide nanoparticle delivery of microRNA-146a for local treatment of acute lung injury , 2021, Nanomedicine: Nanotechnology, Biology and Medicine.

[5]  E. Yahya,et al.  Recent trends in cancer therapy: A review on the current state of gene delivery. , 2021, Life sciences.

[6]  H. Yaghoubi,et al.  Design and fabrication of novel multi-targeted magnetic nanoparticles for gene delivery to breast cancer cells , 2020 .

[7]  V. Thakur,et al.  Nanoparticles as an emerging tool to alter the gene expression: Preparation and conjugation methods , 2020 .

[8]  Chun Wang,et al.  Virus-like nanoparticle as a co-delivery system to enhance efficacy of CRISPR/Cas9-based cancer immunotherapy. , 2020, Biomaterials.

[9]  Yu Nie,et al.  Magnetofection: Magic magnetic nanoparticles for efficient gene delivery , 2020 .

[10]  Jiah Shin Chin,et al.  Localized delivery of CRISPR/dCas9 via layer-by-layer self-assembling peptide coating on nanofibers for neural tissue engineering. , 2020, Biomaterials.

[11]  Jianliang Shen,et al.  Multifunctional magnetic iron oxide nanoparticles: an advanced platform for cancer theranostics , 2020, Theranostics.

[12]  Hongngee Lim,et al.  In-situ surface functionalization of superparamagnetic reduced graphene oxide – Fe3O4 nanocomposite via Ganoderma lucidum extract for targeted cancer therapy application , 2020, Applied Surface Science.

[13]  Bing Yu,et al.  Preparation, surface functionalization and application of Fe3O4 magnetic nanoparticles. , 2020, Advances in colloid and interface science.

[14]  T. Creczynski-Pasa,et al.  Multifunctional hybrid nanoparticles as magnetic delivery systems for siRNA targeting the HER2 gene in breast cancer cells. , 2020, Materials science & engineering. C, Materials for biological applications.

[15]  Zhongyu Li,et al.  Nanoparticle depots for controlled and sustained gene delivery. , 2020, Journal of controlled release : official journal of the Controlled Release Society.

[16]  D. Corey,et al.  Viral vectors for gene delivery to the inner ear , 2020, Hearing Research.

[17]  V. Chandrasekhar,et al.  Functionalized iron oxide nanoparticles conjugate of multi-anchored Schiff’s base inorganic heterocyclic pendant groups: Cytotoxicity studies , 2020 .

[18]  A. Shtil,et al.  Preclinical Evaluation and Clinical Translation of Magnetite-Based Nanomedicines , 2019 .

[19]  M. Darroudi,et al.  Green synthesis of 99mTc-labeled-Fe3O4 nanoparticles using Quince seeds extract and evaluation of their cytotoxicity and biodistribution in rats , 2019, Journal of Molecular Structure.

[20]  A. Allafchian,et al.  Antibacterial magnetic nanoparticles for therapeutics: a review. , 2019, IET nanobiotechnology.

[21]  P. Golkar,et al.  Fabrication and characterization of electrospun Plantago major seed mucilage/PVA nanofibers , 2019, Journal of Applied Polymer Science.

[22]  L. Avilés-Arellano,et al.  How to decrease the agglomeration of magnetite nanoparticles and increase their stability using surface properties , 2019, Colloids and Surfaces A: Physicochemical and Engineering Aspects.

[23]  Gabriela Regina Rosa Souza,et al.  Magnetically responsive hybrid nanoparticles for in vitro siRNA delivery to breast cancer cells. , 2019, Materials science & engineering. C, Materials for biological applications.

[24]  P. A. Shah,et al.  Natural biodegradable polymers based nano‐formulations for drug delivery: A review , 2019, International journal of pharmaceutics.

[25]  P. Cullis,et al.  Lipid-Based DNA Therapeutics: Hallmarks of Non-Viral Gene Delivery. , 2019, ACS nano.

[26]  J. Champion,et al.  Alginate/chitosan microparticles for gastric passage and intestinal release of therapeutic protein nanoparticles , 2019, Journal of controlled release : official journal of the Controlled Release Society.

[27]  D. Ling,et al.  Uniformly sized iron oxide nanoparticles for efficient gene delivery to mesenchymal stem cells , 2018, International journal of pharmaceutics.

[28]  A. Allafchian,et al.  Biocompatible biodegradable polycaprolactone/basil seed mucilage scaffold for cell culture. , 2018, IET nanobiotechnology.

[29]  A. Pannier,et al.  Oral Non-Viral Gene Delivery for Applications in DNA Vaccination and Gene Therapy. , 2018, Current opinion in biomedical engineering.

[30]  F. Alarcón,et al.  Feed pellets containing chitosan nanoparticles as plasmid DNA oral delivery system for fish: In vivo assessment in gilthead sea bream (Sparus aurata) juveniles , 2018, Fish & shellfish immunology.

[31]  A. Allafchian,et al.  Synthesis and characterization of basil seed mucilage coated Fe3O4 magnetic nanoparticles as a drug carrier for the controlled delivery of cephalexin. , 2018, International journal of biological macromolecules.

[32]  S. Bashir,et al.  Quince seed hydrogel (glucuronoxylan): Evaluation of stimuli responsive sustained release oral drug delivery system and biomedical properties , 2018, Journal of Drug Delivery Science and Technology.

[33]  J. C. Zuaznabar‐Gardona,et al.  Iron Oxide Nanoparticles (IONPs) with potential applications in plasmid DNA isolation , 2018 .

[34]  Chun-yan Zhu,et al.  Chinese quince seed gum and poly (N,N-diethylacryl amide-co-methacrylic acid) based pH-sensitive hydrogel for use in drug delivery. , 2018, Carbohydrate polymers.

[35]  P. Ouyang,et al.  Preparation, characterization and evaluation of the immune effect of alginate/chitosan composite microspheres encapsulating recombinant protein of Streptococcus iniae designed for fish oral vaccination , 2018, Fish & shellfish immunology.

[36]  A. V. Oliveira,et al.  Non-viral strategies for ocular gene delivery. , 2017, Materials science & engineering. C, Materials for biological applications.

[37]  W. Vermerris,et al.  Recent Advances in Nanomaterials for Gene Delivery—A Review , 2017, Nanomaterials.

[38]  M. N. Salimi,et al.  Synthesis and characterization of biocompatible Fe3O4 nanoparticles at different pH , 2017 .

[39]  B. Farrugia,et al.  Perlecan and vascular endothelial growth factor‐encoding DNA‐loaded chitosan scaffolds promote angiogenesis and wound healing , 2017, Journal of controlled release : official journal of the Controlled Release Society.

[40]  A. Entezami,et al.  Synthesis and characterization of Fe3O4-PEG-LAC-chitosan-PEI nanoparticle as a survivin siRNA delivery system , 2017, Human & experimental toxicology.

[41]  Xiaoliang Qi,et al.  Amine-functionalized magnetic mesoporous silica nanoparticles for DNA separation , 2016 .

[42]  H. Hosseinzadeh,et al.  Quince seed mucilage magnetic nanocomposites as novel bioadsorbents for efficient removal of cationic dyes from aqueous solutions. , 2015, Carbohydrate polymers.

[43]  B. Stoica,et al.  Study on iron oxide nanoparticles coated with glucose-derived polymers for biomedical applications , 2015 .

[44]  Dilaveez Rehana,et al.  Hydroxy, carboxylic and amino acid functionalized superparamagnetic iron oxide nanoparticles: Synthesis, characterization and in vitro anti-cancer studies , 2015, Journal of Chemical Sciences.

[45]  V. Saikko,et al.  Structural characterization and tribological evaluation of quince seed mucilage , 2014 .

[46]  Eleonore Fröhlich,et al.  The role of surface charge in cellular uptake and cytotoxicity of medical nanoparticles , 2012, International journal of nanomedicine.

[47]  R. Hong,et al.  Preparation and characterization of magnetic gene vectors for targeting gene delivery , 2012 .

[48]  Wenshuai Chen,et al.  Individualization of cellulose nanofibers from wood using high-intensity ultrasonication combined with chemical pretreatments , 2011 .

[49]  Marco Parvis,et al.  In-Process Temperature Mapping System for Industrial Freeze Dryers , 2010, IEEE Transactions on Instrumentation and Measurement.

[50]  Xiguang Chen,et al.  Formation and oral administration of alginate microspheres loaded with pDNA coding for lymphocystis disease virus (LCDV) to Japanese flounder. , 2008, Fish & shellfish immunology.

[51]  T. Nagai,et al.  Preparation and characterization of superparamagnetic iron oxide nanoparticles stabilized by alginate. , 2007, International journal of pharmaceutics.

[52]  Jing Sun,et al.  Synthesis and characterization of biocompatible Fe3O4 nanoparticles. , 2007, Journal of biomedical materials research. Part A.