In situ and real-time authentication of Thunnus species by iKnife rapid evaporative ionization mass spectrometry based lipidomics without sample pretreatment.

Tuna adulteration and mislabeling are serious problem worldwide and have caused economic loss and consumer rights violation. In this study, an electrometric knife (iKnife) coupling rapid evaporative ionization mass spectrometry (REIMS) and a multivariate recognition model were developed and employed for in situ and real-time authentication of four tuna species without sample preparation. The results showed that the lipidomic profiles were successfully acquired and the differences in fatty acids and phospholipids were statistically analyzed to be significant (p < 0.05). The model displayed the superb classification accuracy (>93%) and validation (R2(Y) = 0.992, Q2 = 0.986), and the main contributors of m/z 817.64, m/z 809.68, etc. were screened out to be used as potential biomarkers. Based on this technique, the identity of blind tuna samples could be unambiguously authenticated with the results displayed on a monitor screen directly. This study provided a front-line rapid detection method to prove the authenticity of tuna species.

[1]  M. T. Bottero,et al.  Identification of Four Tuna Species by Means of Real-Time PCR and Melting Curve Analysis , 2007, Veterinary Research Communications.

[2]  A. Amorim,et al.  Tuna fish identification using mtDNA markers , 2017 .

[3]  Yanan Lin,et al.  Structural characteristics of dietary fiber (Vigna radiata L. hull) and its inhibitory effect on phospholipid digestion as an additive in fish floss , 2019, Food Control.

[4]  C. Elliott,et al.  Ambient mass spectrometry based on REIMS for the rapid detection of adulteration of minced meats by the use of a range of additives , 2019, Food Control.

[5]  C. González-López,et al.  NMR Metabolomics as an Effective Tool To Unravel the Effect of Light Intensity and Temperature on the Composition of the Marine Microalgae Isochrysis galbana. , 2019, Journal of agricultural and food chemistry.

[6]  J. Luten,et al.  Identification of fish species after cooking by SDS-PAGE and urea IEF: a collaborative study. , 2000, Journal of agricultural and food chemistry.

[7]  H. Pethybridge,et al.  Lipid and fatty acid dynamics in mature female albacore tuna (Thunnus alalunga) in the western Indian Ocean , 2018, PloS one.

[8]  Hongshun Yang,et al.  Metabolite profiling of Listeria innocua for unravelling the inactivation mechanism of electrolysed water by nuclear magnetic resonance spectroscopy. , 2018, International journal of food microbiology.

[9]  Qing Shen,et al.  Shotgun lipidomics strategy for fast analysis of phospholipids in fisheries waste and its potential in species differentiation. , 2012, Journal of agricultural and food chemistry.

[10]  N. Pérez-Hernández,et al.  1H NMR-based fingerprinting of eleven Mexican Capsicum annuum cultivars. , 2019, Food research international.

[11]  Qing Shen,et al.  In Situ Method for Real-Time Discriminating Salmon and Rainbow Trout without Sample Preparation Using iKnife and Rapid Evaporative Ionization Mass Spectrometry-Based Lipidomics. , 2019, Journal of agricultural and food chemistry.

[12]  Gongshuai Song,et al.  Analysis of flavor change in the industrial production of fungal fermentation based mussel (Mytilus edulis) cooking liquor using a laser irradiation desorption based GC/MS method , 2019, LWT.

[13]  Gongshuai Song,et al.  Real-time assessing the lipid oxidation of prawn (Litopenaeus vannamei) during air-frying by iKnife coupling rapid evaporative ionization mass spectrometry , 2020 .

[14]  G. Mourente,et al.  Trophic links of Atlantic Bluefin tuna (Thunnus thynnus L.) inferred by fatty acid signatures , 2015 .

[15]  Junli Feng,et al.  Zwitterionic hydrophilic interaction solid-phase extraction and multi-dimensional mass spectrometry for shotgun lipidomic study of Hypophthalmichthys nobilis. , 2017, Food chemistry.

[16]  L. Mondello,et al.  Correction to: Use of an “Intelligent Knife” (iknife), Based on the Rapid Evaporative Ionization Mass Spectrometry Technology, for Authenticity Assessment of Pistachio Samples , 2019, Food Analytical Methods.

[17]  C. Fotakis,et al.  NMR metabolic fingerprinting and chemometrics driven authentication of Greek grape marc spirits. , 2016, Food chemistry.

[18]  Zhiyuan Dai,et al.  Rapid determination of caffeoylquinic acid derivatives in Cynara scolymus L. by ultra-fast liquid chromatography/tandem mass spectrometry based on a fused core C18 column. , 2010, Journal of separation science.

[19]  J. Shiao,et al.  Identification of tuna species by a real-time polymerase chain reaction technique , 2012 .

[20]  H. Cheung,et al.  Graphene/TiO2 nanocomposite based solid-phase extraction and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for lipidomic profiling of avocado (Persea americana Mill.). , 2014, Analytica chimica acta.

[21]  Yanan Lin,et al.  Rapid Evaporative Ionization Mass Spectrometry-Based Lipidomics Tracking of Grass Carp ( Ctenopharyngodon idellus) during In Vitro Multiple-Stage Digestion. , 2018, Journal of agricultural and food chemistry.

[22]  Sarah De Saeger,et al.  Rapid evaporative ionization mass spectrometry for high-throughput screening in food analysis: The case of boar taint. , 2017, Talanta.

[23]  C. Jacobsen,et al.  Impact of primary amine group from aminophospholipids and amino acids on marine phospholipids stability: non-enzymatic browning and lipid oxidation. , 2013, Food chemistry.

[24]  Dimitrios S. Nikolopoulos,et al.  A real time metabolomic profiling approach to detecting fish fraud using rapid evaporative ionisation mass spectrometry , 2017, Metabolomics.

[25]  Carmen G Sotelo,et al.  Development of a multiplex PCR-ELISA method for the genetic authentication of Thunnus species and Katsuwonus pelamis in food products. , 2015, Food chemistry.

[26]  M. Pardo,et al.  Identification of commercial canned tuna species by restriction site analysis of mitochondrial DNA products obtained by nested primer PCR , 2004 .

[27]  M. Pardo,et al.  Application of relative quantification TaqMan real-time polymerase chain reaction technology for the identification and quantification of Thunnus alalunga and Thunnus albacares. , 2005, Journal of agricultural and food chemistry.

[28]  C. Elliott,et al.  The feasibility of applying NIR and FT-IR fingerprinting to detect adulteration in black pepper , 2019, Food Control.

[29]  U. Edlund,et al.  Visualization of GC/TOF-MS-based metabolomics data for identification of biochemically interesting compounds using OPLS class models. , 2008, Analytical chemistry.

[30]  R. Karoui,et al.  Identification and quantification of tuna species in canned tunas with sunflower medium by means of a technique based on front face fluorescence spectroscopy (FFFS) , 2019, Food Control.

[31]  A. A. Zynudheen,et al.  Studies on chemical composition of yellowfin tuna (Thunnus albacares, Bonnaterre, 1788) eye , 2017, Journal of Food Science and Technology.

[32]  L. Mondello,et al.  Use of an “Intelligent Knife” (iknife), Based on the Rapid Evaporative Ionization Mass Spectrometry Technology, for Authenticity Assessment of Pistachio Samples , 2018, Food Analytical Methods.

[33]  J. Kinross,et al.  Rapid evaporative ionization mass spectrometry imaging platform for direct mapping from bulk tissue and bacterial growth media. , 2015, Analytical chemistry.

[34]  Zoltan Takats,et al.  Identification of the Species of Origin for Meat Products by Rapid Evaporative Ionization Mass Spectrometry. , 2016, Journal of agricultural and food chemistry.

[35]  G. Szakács,et al.  Shotgun Lipidomic Profiling of the NCI60 Cell Line Panel Using Rapid Evaporative Ionization Mass Spectrometry. , 2016, Analytical chemistry.

[36]  U. Christaki,et al.  Development of a qPCR Method for the Identification and Quantification of Two Closely Related Tuna Species, Bigeye Tuna (Thunnus obesus) and Yellowfin Tuna (Thunnus albacares), in Canned Tuna. , 2017, Journal of agricultural and food chemistry.

[37]  Hongshun Yang,et al.  Metabolomic analysis of energy regulated germination and sprouting of organic mung bean (Vigna radiata) using NMR spectroscopy. , 2019, Food chemistry.