Mechanism of in vivo DNA transport into cells by electroporation: electrophoresis across the plasma membrane may not be involved

Recently, in vivo gene transfer with electroporation (electro‐gene transfer) has emerged as a leading technology for developing nonviral gene therapies and nucleic acid vaccines. The widely hypothesized mechanism is that electroporation induces structural defects in the membrane and provides an electrophoretic force to facilitate DNA crossing the permeabilized membrane. In this study, we have designed a device and experiments to test the hypothesis.

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