Light Intensity-Induced Changes in cab mRNA and Light Harvesting Complex II Apoprotein Levels in the Unicellular Chlorophyte Dunaliella tertiolecta.

During a transition from high growth irradiance (700 micromoles quanta per square meter per second) to low growth irradiance (70 micromoles quanta per square meter per second), the unicellular marine chlorophyte Dunaliella tertiolecta Butcher increases the cellular pool size of the light-harvesting complex of photosystem II (LHC II). We showed that the increase in LHC II apoproteins and in chlorophyll content per cell is preceded by an approximately fourfold increase in cab mRNA. The increase in cab mRNA is detectable within 1.5 hours following a shift from high to low light intensity. An increase in the relative abundance of cab mRNA was also found following a shift from high light to darkness and from high light to low light in the presence of gabaculine, a chlorophyll synthesis inhibitor. However, the LHC II apoproteins did not accumulate in the latter experiments, suggesting that LHC II apoprotein synthesis is coupled to chlorophyll synthesis at or beyond translation. We propose that changes in energy balance brought about by a change in light intensity may control a regulatory factor acting to repress cab mRNA expression in high light.

[1]  P. Falkowski,et al.  ACCLIMATION TO SPECTRAL IRRADIANCE IN ALGAE , 1991 .

[2]  J. Sheen,et al.  Metabolic repression of transcription in higher plants. , 1990, The Plant cell.

[3]  A. Murakami,et al.  Regulation of the Size of Light-Harvesting Antennae in Response to Light Intensity in the Green Alga Chlorella pyrenoidosa , 1989 .

[4]  P. Westhoff,et al.  Light Quality and Irradiance Level Interaction in the Control of Expression of Light-Harvesting Complex of Photosystem 11 , 1989 .

[5]  U. Johanningmeier.  Possible control of transcript levels by chlorophyll precursors in Chlamydomonas. , 1988, European journal of biochemistry.

[6]  P. Falkowski,et al.  Changes in the abundance of individual apoproteins of light-harvesting chlorophyll ab-protein complexes of Photosystem I and II with growth irradiance in the marine chlorophyte Dunaliella tertiolecta , 1988 .

[7]  J. Barber,et al.  Dynamics of Photosystem II and Its Light Harvesting System in Response to Light Changes in the Halotolerant Alga Dunaliella salina. , 1987, Plant physiology.

[8]  Richard S. Williams,et al.  Failure of corn leaves to acclimate to low irradiance. Role of protochlorophyllide reductase in regulating levels of five chlorophyll-binding proteins , 1987 .

[9]  P. Falkowski,et al.  A novel mechanism for regulating the excitation of photosystem II in a green alga , 1987, Nature.

[10]  Paul G. Falkowski,et al.  Growth‐irradiance relationships in phytoplankton1 , 1985 .

[11]  M. Ghirardi,et al.  The mechanism of photosynthetic membrane adaptation to environmental stress conditions: a hypothesis on the role of electron-transport capacity and of ATP/NADPH pool in the regulation of thylakoid membrane organization and function , 1985 .

[12]  P. Falkowski,et al.  Kinetics of adaptation to irradiance in Dunaliella tertiolecta , 1984 .

[13]  T. G. Owens,et al.  Light-Shade Adaptation : TWO STRATEGIES IN MARINE PHYTOPLANKTON. , 1980, Plant physiology.

[14]  P. Falkowski.  Light-Shade Adaptation in Marine Phytoplankton , 1980 .

[15]  N. Boardman.  Comparative photosynthesis of sun and shade plants. , 1977 .

[16]  G. F. Humphrey,et al.  New spectrophotometric equations for determining chlorophylls a, b, c1 and c2 in higher plants, algae and natural phytoplankton , 1975 .

[17]  R. Hiller,et al.  The relationship between chlorophyll b and pigment-protein complex II. , 1974, Biochimica et biophysica acta.

[18]  S. Beale,et al.  Chlorophyll synthesis in chlorella: regulation by degree of light limitation of growth. , 1971, Plant physiology.

[19]  D. Appleman,et al.  Chlorophyll Synthesis in Chlorella II. Effect of Glucose and Light Intensity on the Lag Phase. , 1966, Plant physiology.

[20]  R. Guillard,et al.  Studies of marine planktonic diatoms. I. Cyclotella nana Hustedt, and Detonula confervacea (cleve) Gran. , 1962, Canadian journal of microbiology.

[21]  M. Maloney,et al.  Kinetics of Chlorophyll Accumulation and Formation of Chlorophyll-Protein Complexes during Greening of Chiamydomonas reinhardtii y-1 at 380C' , 2022 .