DNA EXTRACTION FROM RESTING EGGS OF THE CLAM SHRIMP EULIMNADIA TEXANA (BRANCHIOPODA: SPINICAUDATA: LIMNADIIDAE)

Abstract Large branchiopod crustaceans inhabiting ephemeral ponds are well adapted to their highly unpredictable habitat with a life cycle that includes a short-lived adult stage and a long-lived, desiccation-resistant egg stage. One well studied large branchiopod is the clam shrimp Eulimnadia texana, an androdioecious species with populations comprised of males and self-compatible hermaphrodites. Likely due to the harsh environments in which the eggs are found, e.g., dry areas with high heat and high irradiation, extraction of DNA from individual eggs for genetic analyses can be problematic. Here we report a number of modifications that we have tested allowing for increased efficiency and success in extracting high quality DNA from the eggs, nauplii, and adults of E. texana that may prove useful for similar studies of other species of large branchiopods.

[1]  W. Hoeh,et al.  Isolation and characterization of 13 polymorphic microsatellite loci from the clam shrimp Eulimnadia texana (Crustacea: Spinicaudata) , 2004 .

[2]  D. Ott,et al.  Cyst development in the conchostracan shrimp, Eulimnadia texana (Crustacea: Spinicaudata) , 2002, Hydrobiologia.

[3]  B. Mantovani,et al.  A preliminary study in the use of RAPD markers in detecting genetic differences in hatching patterns of Chirocephalus diaphanus Prévost, 1803 (Crustacea: Anostraca) , 2002, Hydrobiologia.

[4]  D. G. George,et al.  A technique for the molecular genetic analysis of Daphnia resting eggs from sub-recent lake sediments , 2002, Journal of Paleolimnology.

[5]  L. Weider,et al.  ‘Ancient’ DNA in the resting egg bank of a microcrustacean can serve as a palaeolimnological database , 2002, Proceedings of the Royal Society of London. Series B: Biological Sciences.

[6]  G. Carvalho,et al.  The interplay between colonization history and gene flow in passively dispersing zooplankton: microsatellite analysis of rotifer resting egg banks , 2002 .

[7]  L. De Meester,et al.  Rapid, local adaptation of zooplankton behavior to changes in predation pressure in the absence of neutral genetic changes , 2001, Proceedings of the National Academy of Sciences of the United States of America.

[8]  S. Weeks,et al.  Maintenance of androdioecy in the freshwater shrimp, Eulimnadia texana: do hermaphrodites need males for complete fertilization? , 2001, Evolutionary Ecology.

[9]  J. Clegg,et al.  Cryptobiosis--a peculiar state of biological organization. , 2001, Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology.

[10]  N. Hairston,et al.  Paleogenetic evidence for a past invasion of Onondaga Lake, New York, by exotic Daphnia curvirostris using mtDNA from dormant eggs , 2000 .

[11]  A. Wood,et al.  “To dryness and beyond” – Preparation for the dried state and rehydration in vegetative desiccation-tolerant plants , 1998, Plant Growth Regulation.

[12]  J. Moorad,et al.  Extraction of DNA from anostracan cysts (Crustacea, Branchiopoda) for use in RAPD-PCR analyses , 1997, Hydrobiologia.

[13]  M. Cunningham,et al.  Anatomical evidence for androdioecy in the clam shrimp Eulimnadia texana , 1997, Hydrobiologia.

[14]  S. Weeks,et al.  Notes on the life history of the clam shrimp, Eulimnadia texana , 1997, Hydrobiologia.

[15]  S. Weeks,et al.  The effects of pond duration on the life history traits of an ephemeral pond crustacean,Eulimnadia texana , 1997, Hydrobiologia.

[16]  L. Rieseberg,et al.  The genetic mechanism of sex determination in the androdioecious flowering plant, Datisca glomerata (Datiscaceae) , 1997, Heredity.

[17]  L. Brendonck.  Diapause, quiescence, hatching requirements: what we can learn from large freshwater branchiopods (Crustacea: Branchiopoda: Anostraca, Notostraca, Conchostraca) , 1996, Hydrobiologia.

[18]  S. Weeks,et al.  The Genetic Mechanism of Sex Determination in the Conchostracan Shrimp Eulimnadia texana , 1993, The American Naturalist.

[19]  R. E. Mclaughlin,et al.  Eulimnadia texana Packard (Conchostraca: Limnadiidae) in Rice Fields in Southwestern Louisiana , 1987 .

[20]  N. Strenth.  Successional Variation of Sex Ratios in Eulimnadia texana Packard (Crustacea, Conchostraca) , 1977 .

[21]  F. Tomita,et al.  ISOLATION AND CHARACTERIZATION OF FLAVONOID COMPOUND FROM FERONIA LIMONIA , 2015 .

[22]  K. Turksen,et al.  Isolation and characterization , 2006 .

[23]  A. Wood,et al.  Desiccation Tolerance in Mosses , 1997 .

[24]  S. Hathaway,et al.  DIVERSIFIED BET-HEDGING AS A REPRODUCTIVE STRATEGY OF SOME EPHEMERAL POOL ANOSTRACANS (BRANCHIOPODA) , 1997 .

[25]  C. Sassaman.  Inbreeding and sex ratio variation in female-biased populations of a clam shrimp, Eulimnadia texana , 1989 .

[26]  M. Blum,et al.  Fundamentals of insect physiology. , 1985 .

[27]  D. Belk.  Functions of the Conchostracan Egg Shell , 1970 .

[28]  a preliminary , 2016 .