Plastids are widespread and ancient in parasites of the phylum Apicomplexa.

Current evidence supports the presence of a non-photosynthetic chloroplast-like organelle in several apicomplexan parasites, including Plasmodium falciparum and Toxoplasma gondii. This apicomplexan organelle, referred to here as the "plastid", may have been acquired through a primary or secondary endosymbiosis of a photosynthetic organism. Alternatively, apicomplexan plastids may have been acquired through several independent endosymbiotic events, as appears to be the case for the acquisition of chloroplasts by dinoflagellates. The likelihood of multiple origins of an apicomplexan plastid is enhanced by the close evolutionary relatedness of apicomplexan and dinoflagellate taxa. In this study, we have tested the hypothesis that apicomplexan plastids are derived from a single ancient ancestor. Two lines of evidence supporting this hypothesis are presented. First, this study supports the widespread presence of plastid DNA in apicomplexan species. Second, the topologies of the phylogenetic trees derived from plastid and nuclear-encoded rRNA gene sequences suggest the co-evolution of the DNAs localised in these two compartments. Taken together, these data support a single ancient lineage for the plastids of parasites in the phylum Apicomplexa.

[1]  S. Barker,et al.  Phylogenetic position of the genus Perkinsus (Protista, Apicomplexa) based on small subunit ribosomal RNA. , 1993, Molecular and biochemical parasitology.

[2]  T. McCutchan,et al.  The Antibiotic Micrococcin Is a Potent Inhibitor of Growth and Protein Synthesis in the Malaria Parasite , 1998, Antimicrobial Agents and Chemotherapy.

[3]  J. Palmer,et al.  Second-hand chloroplasts and the case of the disappearing nucleus. , 1996, Proceedings of the National Academy of Sciences of the United States of America.

[4]  P A Liberator,et al.  Phylogenetic relationships among eight Eimeria species infecting domestic fowl inferred using complete small subunit ribosomal DNA sequences. , 1997, The Journal of parasitology.

[5]  M. Gardner,et al.  Mitochondrial DNA of the human malarial parasite Plasmodium falciparum. , 1988, Molecular and biochemical parasitology (Print).

[6]  P. Allen,et al.  Production of free radical species during Eimeria maxima infections in chickens. , 1997, Poultry science.

[7]  V. Weissig,et al.  Topoisomerase II inhibitors induce cleavage of nuclear and 35-kb plastid DNAs in the malarial parasite Plasmodium falciparum. , 1997, DNA and cell biology.

[8]  G. Cothran,et al.  Phylogenetic relationship of Sarcocystis neurona to other members of the family Sarcocystidae based on small subunit ribosomal RNA gene sequence. , 1994, The Journal of parasitology.

[9]  M. Gardner,et al.  Organisation and expression of small subunit ribosomal RNA genes encoded by a 35-kilobase circular DNA in Plasmodium falciparum. , 1991, Molecular and biochemical parasitology.

[10]  G. McFadden,et al.  Plastids in parasites of humans. , 1997, BioEssays : news and reviews in molecular, cellular and developmental biology.

[11]  R. Page.  Maps between trees and cladistic analysis of historical associations among genes , 1994 .

[12]  R. Iggo,et al.  A rapid PCR fidelity assay. , 1994, Nucleic acids research.

[13]  P. Brindley,et al.  The phylogeny of Neospora caninum. , 1994, Molecular and biochemical parasitology.

[14]  J. Barta,et al.  Phylogenetic analysis of the class Sporozoea (phylum Apicomplexa Levine, 1970): evidence for the independent evolution of heteroxenous life cycles. , 1989, The Journal of parasitology.

[15]  N. Lang-Unnasch,et al.  Phylogeny of the Large Extrachromosomal DNA of Organisms in the Phylum Apicomplexa , 1995, The Journal of eukaryotic microbiology.

[16]  P. Denny,et al.  Evidence for a Single Origin of the 35 kb Plastid DNA in Apicomplexans. , 1998, Protist.

[17]  M. Strath,et al.  Complete gene map of the plastid-like DNA of the malaria parasite Plasmodium falciparum. , 1996, Journal of molecular biology.

[18]  G. McFadden,et al.  Plastids in apicomplexan parasites , 1997 .

[19]  T. G. Smith,et al.  The genus Hepatozoon (Apicomplexa: Adeleina). , 1996, The Journal of parasitology.

[20]  J. E. Hyde,et al.  Green roots of malaria. , 1994, Parasitology today.

[21]  R. Wilson,et al.  Extrachromosomal DNA in the Apicomplexa. , 1997, Microbiology and molecular biology reviews : MMBR.

[22]  Y. Zhao,et al.  Plasmodium falciparum calcium-dependent protein kinase phosphorylates proteins of the host erythrocytic membrane. , 1994, Molecular and biochemical parasitology.

[23]  David S. Roos,et al.  A plastid organelle as a drug target in apicomplexan parasites , 1997, Nature.

[24]  M. Gardner,et al.  Phylogenetic analysis of the rpoB gene from the plastid-like DNA of Plasmodium falciparum. , 1994, Molecular and biochemical parasitology.

[25]  S. B. Needleman,et al.  A general method applicable to the search for similarities in the amino acid sequence of two proteins. , 1970, Journal of molecular biology.

[26]  M. Sogin,et al.  Ribosomal RNA sequences of Sarcocystis muris, Theileria annulata and Crypthecodinium cohnii reveal evolutionary relationships among apicomplexans, dinoflagellates, and ciliates. , 1991, Molecular and biochemical parasitology.

[27]  T. McCutchan,et al.  Inhibition of Plasmodium falciparum Protein Synthesis , 1997, The Journal of Biological Chemistry.

[28]  F. Tekaia,et al.  Molecular characterization of the heat shock protein 90 gene of the human malaria parasite Plasmodium falciparum. , 1994, Molecular and biochemical parasitology.

[29]  J. Wolters,et al.  The troublesome parasites--molecular and morphological evidence that Apicomplexa belong to the dinoflagellate-ciliate clade. , 1991, Bio Systems.

[30]  Geoffrey I. McFadden,et al.  Plastid in human parasites , 1996, Nature.

[31]  C. Howe.  Plastid origin of an extrachromosomal DNA molecule from Plasmodium, the causative agent of malaria. , 1992, Journal of theoretical biology.

[32]  J. Palmer,et al.  A Plastid of Probable Green Algal Origin in Apicomplexan Parasites , 1997, Science.

[33]  J. Barta,et al.  Evolutionary relationships of avian Eimeria species among other Apicomplexan protozoa: monophyly of the apicomplexa is supported. , 1991, Molecular biology and evolution.

[34]  N. Lang-Unnasch,et al.  Purification and properties of Plasmodium falciparum malate dehydrogenase. , 1992, Molecular and biochemical parasitology.