Detection Limits for Protein after Electrophoresis on Cellulose Acetate

The detection limits for protein, when stained with nigrosine after electrophoresis on cellulose acetate, were studied and shown to be: albumin, 3·0 ng; transferrin, 0·8 ng; and Bence-Jones protein, 0·8 ng. This was less than one-hundredth of the previously reported levels. Immuno-fixation, although useful for identification, did not lower the detection limit for Bence-Jones protein.