Dark-field digital holographic microscopy to investigate objects that are nanosized or smaller than the optical resolution.

We describe a transmission dark-field digital holographic microscope based on a Mach-Zehnder configuration for the detection of nanosize objects or objects smaller than the optical resolution limit. An optical stop adequately placed in the object beam removes the nondiffracted beam while keeping the light scattered by the object. This configuration combines an improved detection of objects smaller than the optical resolution with the refocusing capability yielded by digital holography. A theoretical analysis and an experimental demonstration are provided.

[1]  Qiao Hu,et al.  Focus detection from digital in-line holograms based on spectral l1 norms. , 2007, Journal of the Optical Society of America. A, Optics, image science, and vision.

[2]  E. Cuche,et al.  Digital holographic microscopy: a noninvasive contrast imaging technique allowing quantitative visualization of living cells with subwavelength axial accuracy. , 2005, Optics letters.

[3]  Thomas Kreis,et al.  Digital holographic interference-phase measurement using the Fourier-transform method , 1986 .

[4]  Peter Klages,et al.  Digital in-line holographic microscopy. , 2006 .

[5]  Ichirou Yamaguchi,et al.  Phase-shifting digital holography , 1997 .

[6]  Mokrane Malek,et al.  Digital in-line holography: influence of the shadow density on particle field extraction. , 2004, Optics express.

[7]  H. Meng,et al.  Digital holographic particle validation via complex wave. , 2007, Applied optics.

[8]  M H Jericho,et al.  Tracking particles in four dimensions with in-line holographic microscopy. , 2003, Optics letters.

[9]  Bahram Javidi,et al.  Depth-independent segmentation of macroscopic three-dimensional objects encoded in single perspectives of digital holograms. , 2007, Optics letters.

[10]  E. Cuche,et al.  Digital holography for quantitative phase-contrast imaging. , 1999, Optics letters.

[11]  Gilles Tessier,et al.  Heterodyne holographic microscopy of gold particles. , 2007, Optics letters.

[12]  Gabriel Popescu,et al.  Erythrocyte structure and dynamics quantified by Hilbert phase microscopy. , 2005, Journal of biomedical optics.

[13]  Lukas Novotny,et al.  Real-time and background-free detection of nanoscale particles. , 2006, Physical review letters.

[14]  Christian Depeursinge,et al.  Total aberrations compensation in digital holographic microscopy with a reference conjugated hologram. , 2006, Optics express.

[15]  Four-dimensional dynamic flow measurement by holographic particle image velocimetry. , 2005, Applied optics.

[16]  Christophe Ducottet,et al.  Direct extraction of the mean particle size from a digital hologram. , 2006, Applied optics.

[17]  Suppression of the Moiré effect in sub-picosecond digital in-line holography. , 2007, Optics express.

[18]  Haw Yang,et al.  Progress in single-molecule tracking spectroscopy , 2008 .

[19]  P. Ferraro,et al.  Direct full compensation of the aberrations in quantitative phase microscopy of thin objects by a single digital hologram , 2007 .