Amplified detection of protein cancer biomarkers using DNAzyme functionalized nanoprobes.

The high sensitive immunoassay of protein cancer biomarkers is achieved by using of peroxidase-mimicking DNAzyme heavily functionalized gold nanoparticles as catalytic probe.

[1]  Chad A Mirkin,et al.  Multiplexed detection of protein cancer markers with biobarcoded nanoparticle probes. , 2006, Journal of the American Chemical Society.

[2]  Itamar Willner,et al.  DNAzymes for sensing, nanobiotechnology and logic gate applications. , 2008, Chemical Society reviews.

[3]  Jun-Jie Zhu,et al.  Horseradish peroxidase-functionalized gold nanoparticle label for amplified immunoanalysis based on gold nanoparticles/carbon nanotubes hybrids modified biosensor. , 2008, Biosensors & bioelectronics.

[4]  C. Mirkin,et al.  Nanoparticle-Based Bio-Bar Codes for the Ultrasensitive Detection of Proteins , 2003, Science.

[5]  J. Storhoff,et al.  Selective colorimetric detection of polynucleotides based on the distance-dependent optical properties of gold nanoparticles. , 1997, Science.

[6]  Itamar Willner,et al.  DNAzyme-Functionalized Au Nanoparticles for the Amplified Detection of DNA or Telomerase Activity , 2004 .

[7]  Feng Yan,et al.  Biomedical and clinical applications of immunoassays and immunosensors for tumor markers , 2007 .

[8]  Chad A Mirkin,et al.  Raman dye-labeled nanoparticle probes for proteins. , 2003, Journal of the American Chemical Society.

[9]  Yi Lu,et al.  Smart nanomaterials inspired by biology: dynamic assembly of error-free nanomaterials in response to multiple chemical and biological stimuli. , 2007, Accounts of chemical research.

[10]  Itamar Willner,et al.  Amplified DNA detection by electrogenerated biochemiluminescence and by the catalyzed precipitation of an insoluble product on electrodes in the presence of the doxorubicin intercalator. , 2002, Angewandte Chemie.

[11]  Itamar Willner,et al.  Catalytic beacons for the detection of DNA and telomerase activity. , 2004, Journal of the American Chemical Society.

[12]  M. Ferrari.  Cancer nanotechnology: opportunities and challenges , 2005, Nature Reviews Cancer.

[13]  P. Witting,et al.  The peroxidase activity of a hemin--DNA oligonucleotide complex: free radical damage to specific guanine bases of the DNA. , 2001, Journal of the American Chemical Society.

[14]  Yi Xiao,et al.  Amplified chemiluminescence surface detection of DNA and telomerase activity using catalytic nucleic acid labels. , 2004, Analytical chemistry.

[15]  Dianping Tang,et al.  In situ amplified electrochemical immunoassay for carcinoembryonic antigen using horseradish peroxidase-encapsulated nanogold hollow microspheres as labels. , 2008, Analytical chemistry.

[16]  Yingfu Li,et al.  DNA-enhanced peroxidase activity of a DNA-aptamer-hemin complex. , 1998, Chemistry & biology.

[17]  Tao Li,et al.  Enhanced catalytic DNAzyme for label-free colorimetric detection of DNA. , 2007, Chemical communications.

[18]  C. Mirkin,et al.  Nanoparticle-based detection in cerebral spinal fluid of a soluble pathogenic biomarker for Alzheimer's disease. , 2005, Proceedings of the National Academy of Sciences of the United States of America.

[19]  Chunhai Fan,et al.  Enzyme‐Based Multi‐Component Optical Nanoprobes for Sequence‐ Specific Detection of DNA Hybridization , 2008 .

[20]  I. Willner,et al.  Amplified analysis of low-molecular-weight substrates or proteins by the self-assembly of DNAzyme-aptamer conjugates. , 2007, Journal of the American Chemical Society.

[21]  E. Wang,et al.  Chemiluminescence thrombin aptasensor using high-activity DNAzyme as catalytic label. , 2008, Chemical communications.

[22]  I. Willner,et al.  Magnetically amplified DNA assays (MADA): sensing of viral DNA and single-base mismatches by using nucleic acid modified magnetic particles. , 2003, Angewandte Chemie.