Characterization of natural nanostructured hydroxyapatite obtained from the bones of Brazilian river fish

In this study, the characterization of the optical properties of natural hydroxyapatite (HAp) [Ca10(PO4)6(OH)2] is discussed. In the first stage of the experiment, natural HAp was processed from the bones of Brazilian river fish such as pintado (Pseudoplatystoma corruscans), jau (Paulicea lutkeni), and cachara (Pseudoplatystoma fasciatum). The bones were calcined at 900°C for different amounts of time (4–12h) and reduced to powder using two different milling times (2 and 4h) in a high-energy ball mill, in order to determine the best procedure for obtaining natural nanostructured HAp powder for the study. In the second stage, material calcined for 8h was milled for 2, 4, 8, and 16h. The techniques of photoacoustic spectroscopy, scanning electron microscopy, and flame atomic absorption spectrometry were applied to characterize these samples. The O–H stretching shown by photoacoustic spectroscopy was correlated to the HAp nanostructure. Structural analysis indicated a Ca∕P ratio close to 1.67 (∼1.64±0.04) an...

[1]  A. W. Nolle,et al.  Quantitative study of a photoacoustic system for powdered samples , 1977 .

[2]  R. Friedman,et al.  Photoacoustic spectroscopy of particulate solids , 1980 .

[3]  Enhancement of photoacoustic signals from condensed materials in the presence of volatile liquids: Influence of optical absorption coefficient, particle size, length of the gas phase and chopping frequency , 1985 .

[4]  Reginald H. Wilson,et al.  Effects of particle size on quantitative photoacoustic spectroscopy using a gas-microphone cell , 1987 .

[5]  D. Williams,et al.  Tissue-biomaterial interactions , 1987 .

[6]  J. Ong,et al.  Calcium phosphate coatings for medical and dental implants , 1993 .

[7]  W. Landis,et al.  Atomic force microscopy imaging of hydroxyapatite , 1993 .

[8]  Yu.,et al.  Erratum: Photoacoustic investigation of the quantum size effect and thermal properties in ZrO2 nanoclusters , 1994, Physical review. B, Condensed matter.

[9]  T. Kokubo,et al.  Ultrastructural study of an apatite layer formed by a biomimetic process and its bonding to bone. , 1996, Biomaterials.

[10]  K. Gonsalves,et al.  Preparation and characterization of thermally stable nanohydroxyapatite , 1997, Journal of materials science. Materials in medicine.

[11]  I. Rehman,et al.  Characterization of hydroxyapatite and carbonated apatite by photo acoustic FTIR spectroscopy , 1997, Journal of materials science. Materials in medicine.

[12]  C. Doyle,et al.  Plasma sprayed hydroxyapatite coatings on titanium substrates. Part 1: Mechanical properties and residual stress levels. , 1998, Biomaterials.

[13]  Jingwei Xu,et al.  FT-Raman and high-pressure infrared spectroscopic studies of dicalcium phosphate dihydrate (CaHPO42H2O) and anhydrous dicalcium phosphate (CaHPO4) , 1999 .

[14]  B. Nies,et al.  Chemical and physicochemical characterization of porous hydroxyapatite ceramics made of natural bone. , 2000, Biomaterials.

[15]  Q. Shen,et al.  Characterization of Electronic States of TiO2 Powders by Photoacoustic Spectroscopy , 2000 .

[16]  Mariano Perez-Amor,et al.  Production of calcium phosphate coatings on Ti6Al4V obtained by Nd:yttrium–aluminum–garnet laser cladding , 2001 .

[17]  Yue Xijuan,et al.  Size-dependent optical properties of nanocrystalline CeO2:Er obtained by combustion synthesis , 2001 .

[18]  E. Chassot,et al.  Differentiation of biological hydroxyapatite compounds by infrared spectroscopy, x-ray diffraction and extended x-ray absorption fine structure , 2001 .

[19]  A. F. Rubira,et al.  The photoacoustic spectroscopy applied in the characterization of the cross-linking process in polymeric materials , 2002 .

[20]  I. M. Kalogeras,et al.  Axially dependent dielectric relaxation response of natural hydroxyapatite single crystals , 2002 .

[21]  A. Sombra,et al.  Optical properties of hydroxyapatite obtained by mechanical alloying , 2002 .

[22]  The size effect in the AgOx-type super-resolution near-field structure , 2002 .

[23]  M. Baesso,et al.  On the application of the photoacoustic methods for the determination of thermo-optical properties of polymers , 2002 .

[24]  T. Vaimakis,et al.  Preparation of hydroxyapatite via microemulsion route. , 2003, Journal of colloid and interface science.

[25]  George Georgiou,et al.  Calcium phosphates and glass composite coatings on zirconia for enhanced biocompatibility. , 2004, Biomaterials.

[26]  D. Yuan,et al.  Synthesis and characterization of lead phosphate hydroxyapatite nanoparticles , 2004 .

[27]  Jonathan C Knowles,et al.  Hydroxyapatite/poly(epsilon-caprolactone) composite coatings on hydroxyapatite porous bone scaffold for drug delivery. , 2004, Biomaterials.

[28]  A. Manivannan,et al.  Structural investigations of synthetic ferrihydrite nanoparticles doped with Si , 2004 .

[29]  H. Zreiqat,et al.  The modulation of osteogenesis in vitro by calcium titanium phosphate coatings. , 2004, Biomaterials.

[30]  P. Fratzl,et al.  Size-controlled hydroxyapatite nanoparticles as self-organized organic-inorganic composite materials. , 2005, Biomaterials.

[31]  Hyoun‐Ee Kim,et al.  Improvement in biocompatibility of ZrO2-Al2O3 nano-composite by addition of HA. , 2005, Biomaterials.

[32]  W. R. Weinand,et al.  Effect of the Calcination Time of Fish Bones in the Synthesis of Hydroxyapatite , 2005 .

[33]  M. Jelínek,et al.  Biological properties of titanium implants covered with hydroxyapatite and zirconia layers by pulsed laser: In vitro study , 2006 .