Quality of Cut Lettuce Treated by Heat Shock: Prevention of Enzymatic Browning, Repression of Phenylalanine Ammonia-lyase Activity, and Improvement on Sensory Evaluation during Storage

Stored cut lettuce gradually turns brown on the cut section after several days of storage, because cutting induces phenylalanine ammonia-lyase (PAL) activity, the biosynthesis of polyphenol is promoted, and the polyphenols are oxidized by polyphenol oxidase. Here, the effect of heat shock treatment at 50°C for 90 s on the quality of cut lettuce during cold storage was examined. The heat shock treatment significantly repressed the induction of PAL activity and phenolics accumulation in cut lettuce during storage, and prevented the browning of cut lettuce. Ascorbic acid content was not affected by the heat shock treatment. The sensory analysis showed that the organoleptic quality of cut lettuce treated by heat shock was significantly better than that of the control cut lettuce. These results show that heat shock treatment is useful for prolonging the shelf life of cut lettuce.

[1]  Larry R. Beuchat,et al.  Survival and growth of Escherichia coli O157:H7 inoculated onto cut lettuce before or after heating in chlorinated water, followed by storage at 5 or 15°C , 2001 .

[2]  Mikal E. Saltveit,et al.  Wound induced changes in phenolic metabolism and tissue browning are altered by heat shock , 2000 .

[3]  Alfred M. Mayer,et al.  Polyphenol oxidases in plants , 1979 .

[4]  R. Brackett,et al.  Survival and growth of Escherichia coli O157:H7 inoculated onto cut lettuce before or after heating in chlorinated water, followed by storage at 5 or 15 degrees C. , 2001, Journal of food protection.

[5]  J. Zoń,et al.  Inhibitors of Phenylalanine Ammonia-Lyase: 2-Aminoindan-2-phosphonic Acid and Related Compounds. , 1992 .

[6]  H. Hyodo,et al.  Induction of phenylalanine ammonia-lyase and increase in phenolics in lettuce leaves in relation to the development of russet spotting caused by ethylene. , 1978, Plant physiology.

[7]  A. Kader,et al.  Effects of CO2 on Total Phenolics, Phenylalanine Ammonia Lyase, and Polyphenol Oxidase in Lettuce Tissue , 1985, Journal of the American Society for Horticultural Science.

[8]  Mikal E. Saltveit,et al.  Effect of Intensity and Duration of Heat-shock Treatments on Wound-induced Phenolic Metabolism in Iceberg Lettuce , 1997 .

[9]  M. Saltveit,et al.  Plant hormone interaction and phenolic metabolism in the regulation of russet spotting in iceberg lettuce. , 1988, Plant physiology.

[10]  A. Yamasaki,et al.  Component Analysis of Vegetables for Chemical Breeding. (Part 1). Comparison of Components between Cultivars of Lettuce (Lactuca sativa L.). , 1996 .

[11]  M. Saltveit,et al.  Phenylalanine ammonia lyase inhibitors control browning of cut lettuce , 1998 .

[12]  H. Heimdal,et al.  Biochemical changes and sensory quality of shredded and MA-packaged iceberg lettuce , 1995 .

[13]  R. Brackett,et al.  Mild heat treatment of lettuce enhances growth of Listeria monocytogenes during subsequent storage at 5°C or 15°C , 2002 .

[14]  容常 村田,et al.  市販リンゴの酵素的褐変 : 褐変,ポリフェノール含量ポリフェノール酸化酵素の関係 , 1995 .

[15]  Francisco Artés,et al.  Effect of selected browning inhibitors on phenolic metabolism in stem tissue of harvested lettuce , 1997 .

[16]  L. Vámos-Vigyázó Prevention of Enzymatic Browning in Fruits and Vegetables: A Review of Principles and Practice , 1995 .

[17]  R. Brackett,et al.  Mild heat treatment of lettuce enhances growth of Listeria monocytogenes during subsequent storage at 5 degrees C or 15 degrees C. , 2002, Journal of Applied Microbiology.

[18]  D. Scheel,et al.  Physiology and Molecular Biology of Phenylpropanoid Metabolism , 1989 .

[19]  M. Saltveit,et al.  Wound-induced phenylalanine ammonia lyase activity: factors affecting its induction and correlation with the quality of minimally processed lettuces , 1996 .

[20]  E. Vierling The Roles of Heat Shock Proteins in Plants , 1991 .

[21]  John R. Whitaker,et al.  The biochemistry and control of enzymatic browning , 1995 .

[22]  H Hisaminato,et al.  Relationship between the Enzymatic Browning and Phenylalanine Ammonia-lyase Activity of Cut Lettuce, and the Prevention of Browning by Inhibitors of Polyphenol Biosynthesis , 2001, Bioscience, biotechnology, and biochemistry.

[23]  Catherine Barry-Ryan,et al.  Ascorbic Acid Retention in Shredded Iceberg Lettuce as Affected by Minimal Processing , 1999 .