Anti-bensulfuron methyl monoclonal antibody production and BSM-detecting indirect competitive enzyme-linked immunoassay establishment

Bensulfuron methyl (BSM) is widely used for agricultural purposes and has raised health concerns, as well as ecological problems. Immunoassay would be one of the most advantaged measurements compared with traditional methods for BSM detection and measurement. In order to develop indirect competitive enzyme-linked immunoassay (icELISA), the anti-BSM monoclonal antibody (anti-BSM MAb) was produced. For the MAb production, BSM was conjugated to OVA and injected to mice with Freud's adjuvant for immunisation. Antiserum screening has revealed successful immunising. One stable hybridoma cell line (2H1) was obtained through cell fusion between spleen cells of immunised mouse and SP 2/0 cells. The MAb secreted by 2H1 cells was of high affinity and sensitivity, as well as specificity to BSM. Then the protocol of the icELISA and standard curve for BSM measurement was made and examined by controlled application. Significantly, the application has exhibited 96.530%–107.2% recovery of BSM. The produced MAb and established immunoassay may facilitate the measurement of BSM and herein help to ensure food safety and regulate environmental protection.

[1]  Chunxia Ding,et al.  [Simultaneous determination of bensulfuron-methyl and mefenacet residues in paddy field using high performance liquid chromatography]. , 2013, Se pu = Chinese journal of chromatography.

[2]  A. Abad‐Somovilla,et al.  Exploring alternative hapten tethering sites for high-affinity anti-picoxystrobin antibody generation. , 2011, Analytical biochemistry.

[3]  A. Abad‐Somovilla,et al.  Hapten synthesis, monoclonal antibody generation, and development of competitive immunoassays for the analysis of picoxystrobin in beer. , 2010, Analytica chimica acta.

[4]  Zuping He,et al.  Generation and characterization of a monoclonal antibody to penicillic acid from Penicillium cyclopium. , 2010 .

[5]  A. Abad‐Somovilla,et al.  Production and characterization of monoclonal antibodies specific to the strobilurin pesticide pyraclostrobin. , 2008, Journal of agricultural and food chemistry.

[6]  Zonghui Yuan,et al.  Development of an indirect competitive ELISA for the detection of furazolidone marker residue in animal edible tissues. , 2008, Journal of agricultural and food chemistry.

[7]  W. Liu,et al.  Preparation of polyclonal antibodies to a derivative of 1-aminohydantoin (AHD) and development of an indirect competitive ELISA for the detection of nitrofurantoin residue in water. , 2007, Journal of agricultural and food chemistry.

[8]  Á. Maquieira,et al.  Synthesis of haptens and development of a sensitive immunoassay for tetracycline residues. Application to honey samples. , 2007, Analytica chimica acta.

[9]  B. Hammock,et al.  Robust and sensitive monoclonal enzyme-linked immunosorbent assay for the herbicide molinate. , 2004, Journal of agricultural and food chemistry.

[10]  C. Sabater,et al.  Effects of bensulfuron-methyl and cinosulfuron on growth of four freshwater species of phytoplankton. , 2002, Chemosphere.

[11]  John R. Crowther,et al.  The ELISA Guidebook , 2000, Methods in Molecular Biology™.

[12]  Liping Wei,et al.  Determination and prediction of partition coefficient and toxicity for sulfonylurea herbicides and their degradation products , 1999 .

[13]  Liping Wei,et al.  The effects of three sulfonylurea herbicides and their degradation products on the green algae Chlorella pyrenoidosa , 1998 .

[14]  K. Langeland.  Hydrilla tuber formation in response to single and sequential bensulfuron methyl exposures at different times , 1996, Hydrobiologia.

[15]  J. Goding,et al.  Monoclonal Antibodies: Principles and Practice , 1996 .

[16]  B M Kaufman,et al.  Immunoassay of pesticides: an update. , 1995, Journal of AOAC International.

[17]  R. Mumma,et al.  Effects of hapten structure and bridging groups on antisera specificity in parathion immunoassay development. , 1982, Journal of agricultural and food chemistry.

[18]  M. Raje,et al.  Synthesis and characterization of hapten-protein conjugates for antibody production against small molecules. , 2004, Bioconjugate chemistry.

[19]  Kiwamu Fujita.  Effect of Soil Application of Several Herbicides on the Initial Growth of Direct Seeded Rice Sown at Different Depths under Flooded Paddy Conditions. , 1999 .

[20]  K. Armbrust,et al.  Surface water monitoring survey for bensulfuron methyl applied in paddy fields , 1998 .

[21]  M. Hewedi,et al.  Monoclonal antibody‐based ELISA for the detection of ethyl parathion , 1994 .

[22]  J. Hay.  Chemistry of sulfonylurea herbicides , 1990 .