Dengue virus-specific memory T cell responses in human volunteers receiving a live attenuated dengue virus type 2 candidate vaccine.

A live attenuated dengue virus type 2 candidate vaccine (16681-PDK53) was evaluated in a phase I trial in 10 nonimmune adult volunteers. The dengue virus-specific memory T cell responses were analyzed as part of this study. Dengue virus-specific T cell proliferative responses were observed in all subjects after stimulating their peripheral blood mononuclear cells with live viruses or noninfectious viral antigens. The highest proliferative response was against dengue virus type 2, although cross-reactivity with other flaviviruses was detected to a lesser degree in some subjects. Dengue virus type 2-specific CD4+ and CD8+ cytotoxic T lymphocytes were generated in all vaccinees. This study investigated whether the candidate vaccine was efficacious in inducing dengue virus-specific CD4+ and CD8+ T cell memory after a single immunization in nonimmune recipients.

[1]  A. Nisalak,et al.  Activation of T lymphocytes in dengue virus infections. High levels of soluble interleukin 2 receptor, soluble CD4, soluble CD8, interleukin 2, and interferon-gamma in sera of children with dengue. , 1991, The Journal of clinical investigation.

[2]  F. Ennis,et al.  Dengue virus-specific, human CD4+ CD8- cytotoxic T-cell clones: multiple patterns of virus cross-reactivity recognized by NS3-specific T-cell clones , 1991, Journal of virology.

[3]  S. Halstead.  Global epidemiology of dengue hemorrhagic fever. , 1990, The Southeast Asian journal of tropical medicine and public health.

[4]  F. Ennis,et al.  Dengue virus-specific human T cell clones. Serotype crossreactive proliferation, interferon gamma production, and cytotoxic activity , 1989, The Journal of experimental medicine.

[5]  A. Nisalak,et al.  Human T cell responses to dengue virus antigens. Proliferative responses and interferon gamma production. , 1989, The Journal of clinical investigation.

[6]  F. Ennis,et al.  Induction of interferon alpha from human lymphocytes by autologous, dengue virus-infected monocytes , 1987, The Journal of experimental medicine.

[7]  F. Ennis,et al.  Lysis of dengue virus-infected cells by natural cell-mediated cytotoxicity and antibody-dependent cell-mediated cytotoxicity , 1984, Journal of virology.

[8]  F. Ennis,et al.  Dengue virus-specific, human CD4+ cytotoxic T lymphocytes generated in short-term culture. , 1993, Viral immunology.

[9]  倉根 一郎.  Dengue virus-specific human T cell clones : serotype cross-reactive proliferation, interferon gamma production and cytotoxic activity , 1992 .

[10]  S. Yoksan,et al.  Immunization with a live attenuated dengue-2-virus candidate vaccine (16681-PDK 53): clinical, immunological and biological responses in adult volunteers. , 1987, Bulletin of the World Health Organization.

[11]  I. Kurane,et al.  Cytotoxic T Lymphocytes , 2022 .